Mass Spectrometry of Fatty Acid Derivatives
These pages are tutorials that deal with the application of mass spectrometry (MS) with electron-impact ionization, and linked to gas chromatography (GC), for the determination of the structures of fatty acids. They tend to be largely practical as opposed to mechanistic accounts. Although methyl esters are used almost universally for GC separations and often for MS, they afford limited structural information only. On the other hand, certain nitrogen-containing derivatives, especially pyrrolidides, 4,4‑dimethyloxazoline (DMOX) derivatives and 3‑pyridylcarbinol ('picolinyl') esters, give much more specific data as discussed in the attached web pages in which more than 735 mass spectra are illustrated (not counting the Archive sections with these and a further 1500 spectra). Spectra of some selected other lipids are also discussed. Whenever new information and mass spectra become available, these web pages will be updated.
If you are new to the subject, you may wish to first consult -
In the following section, web pages describe the virtues of the different types of derivative together with practical methods for their preparation. Some ancillary practical techniques and other methods that aid analysis are also discussed.
Other Main Topics
Documents are grouped into five main sections according to the nature of the derivatives used and chemical structures, and they can be addressed from the following links -
Archives of Mass Spectra - All our mass spectra (2227 in total) are available here for study or reference, but without interpretation, in Archives of methyl esters - 3-pyridylcarbinol esters - 4,4'-dimethyloxazolines - pyrrolidides - alkyl esters - other lipids.
Relevant Literature Surveys - up to 1989 - or - 1990 to 1999 - or - 2000 to 2009 - or - 2010 onwards - or - review articles. There are additional literature survey topics in most of the major sections
Recommended Book: Christie, W.W. and Han, X. Lipid Analysis - Isolation, Separation, Identification and Lipidomic Analysis (4th edition), 446 pages (Oily Press, Woodhead Publishing and now Elsevier) (2010) - at Science Direct.
|Credits/disclaimer/contact||Updated: June 10th, 2021||Author: William W. Christie|