Mass Spectrometry of Fatty Acid Derivatives


These pages are tutorials that deal with the application of mass spectrometry (MS) with electron-impact ionization and linked to gas chromatography (GC) for the determination of the structures of fatty acids. They tend to be largely practical as opposed to mechanistic accounts. Although methyl esters are used almost universally for GC separations and often for MS, their mass spectra afford limited structural information only. On the other hand, certain nitrogen-containing derivatives, especially pyrrolidides, 4,4‑dimethyloxazoline (DMOX) derivatives, and 3‑pyridylcarbinol ('picolinyl') esters, give much more informative data as discussed in the attached web pages in which more than 735 mass spectra are illustrated (not counting the Archive sections with these and a further 1500 spectra). Spectra of some selected other lipids are also discussed but only those encountered in our research efforts and for which we have spectra. Whenever new information and mass spectra become available, these web pages will be updated.

Recommended Book: Christie, W.W. and Han, X. Lipid Analysis - Isolation, Separation, Identification and Lipidomic Analysis (4th edition), 446 pages (Oily Press, Woodhead Publishing and now Elsevier) (2010) - at Science Direct.

Updated: August 31st, 2022 © Author: William W. Christie LipidWeb icon
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