Experimental data

LIPID MAPS NASH Study

LIPID MAPS is currently conducting a large study to better understand the progression of liver disease. The study is Institutional Review Board (IRB)-approved, and is designed to include data from a cohort of 80 patients, separated by group: normal, steatosis, steatohepatitis, cirrhosis. Data will be posted here after the study has been completed.

Lipidomics studies on human plasma

Centralized studies on macrophages

We performed both lipidomics studies and microarray analysis of RAW 264.7 cells and primary macrophages, using various treatments. These studies were conducted using standardized protocols for isolation, expansion and analysis of primary macrophages and macrophage cell lines. Central preparation of cells enable LIPID MAPS lipidomics cores to characterize lipids under the most uniform conditions possible and allow results in one core unit to be directly compared to results in other core units.

Lipidomics studies on macrophages

Microarray analysis of macrophages

Subcellular fractionation studies on macrophages

Measurements of lipid quanitities present in various cell fractions (cytoplasm, ER, mitochondria, nucleus, plasma membrane) for control and treatment with Kdo2-Lipid A

logo LIPID MAPS is funded by a grant from the National Institute of General Medical Sciences of the National Institutes of Health.