Experimental data
Lipidomics studies on human plasma
Centralized studies on macrophages
We performed both lipidomics studies and macrophage analysis of RAW 264.7 cells and primary macrophages, using various treatments. These studies were conducted using standardized protocols for isolation, expansion and analysis of primary macrophages and macrophage cell lines. Central preparation of cells enable LIPID MAPS lipidomics cores to characterize lipids under the most uniform conditions possible and allow results in one core unit to be directly compared to results in other core units.
Lipidomics studies on macrophages
- Primary macrophages
- Thioglycolate-elicited peritoneal macrophages (TGEM)
- Bone marrow-derived macrophages (BMDM)
- Compare results from TGEM and BMDM treated with Kdo2-Lipid A (3 time points: 0,6,24 hrs)
- Compare results from RAW, TGEM, and BMDM for sterols treated with Kdo2-Lipid A (full time course: 8 time points)
Microarray analysis of macrophages
- RAW 264.7 cells
- Kdo2-Lipid A time course experiments
- Compactin/Kdo2-Lipid A time course experiments (2 time points: 12, 24hrs)
- Compactin/Kdo2-Lipid A time course experiments (full time course: 7 time points)
- Primary macrophages
- Thioglycolate-elicited peritoneal macrophages (TGEM)
- Bone marrow-derived macrophages (BMDM)
Subcellular fractionation studies on macrophages
Measurements of lipid quanitities present in various cell fractions (cytoplasm, ER, mitochondria, nucleus, plasma membrane) for control and treatment with
Kdo2-Lipid A
- RAW 264.7 cells
