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Microarray analysis of foam cells in wild-type and LDLR(-/-) mice

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Introduction Experimental design Peritoneal macrophages were obtained from thioglycolate-stimulated wild-type and low-density lipoprotein receptor knockout (LDLR(-/-)) mice maintained on normal and Western (High-fat, high cholesterol) diets according to protocols PP0000003000 and PP0000003100 (pdf). Measurements were taken for: (i) Wild-type mouse/Normal diet; (ii) Wild-type mouse/High-cholesterol diet; (iii)LDLR(-/-) mouse/Normal diet; (iv) LDLR( -/-) mouse/High-cholesterol diet. Array Design 45,214 spots on the array 41,034 data spots 38,838 unique probes (6428 probes have no gene id) 21,609 unique mouse genes Each gene was represented by 1 - 9 probes Each probe was spotted 1 or 10 times Data Normalization Data normalization was performed as described in J Mol Endocrinol Sasik et al. 33 (1): 1 (PDF)