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LIPID Metabolites And Pathways Strategy

Lipidomics Studies on Macrophages

Compactin/Kdo2-Lipid A Experiments

Experiments were conducted with RAW264.7 cells fed 10% fetal calf serum, as per protocol PP0000002400 (pdf). Measurements were taken at 0, 12, and 24hrs under 4 treatment conditions: (i) compactin, (ii) Kdo2-Lipid A, (iii) compactin + Kdo2-Lipid A. and (iv) control. Three separate experiments (biological replicates) were carried out, and MS measurements were performed in triplicate (technical replicates).

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Averages of replicates, by analyte category