LMPD Database


UniProt Annotations

Entry Information
Gene Namearachidonate 12-lipoxygenase, 12R type
Protein EntryLX12B_HUMAN
UniProt IDO75342
Comment typeDescription
Catalytic ActivityArachidonate + O(2) = (5Z,8Z,10E,14Z)-(12R)- 12-hydroperoxyicosa-5,8,10,14-tetraenoate.
CofactorName=Fe cation; Xref=ChEBI
DiseaseIchthyosis, congenital, autosomal recessive 2 (ARCI2) [MIM
FunctionNon-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12R)- hydroperoxyeicosatetraenoic acid/(12R)-HPETE and minor stereoisomers. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins. {ECO
InteractionQ9BYJ1:ALOXE3; NbExp=2; IntAct=EBI-6925925, EBI-6925949;
PathwayLipid metabolism; hydroperoxy eicosatetraenoic acid biosynthesis.
PathwayLipid metabolism; sphingolipid metabolism.
SimilarityBelongs to the lipoxygenase family.
SimilarityContains 1 lipoxygenase domain. {ECO
SimilarityContains 1 PLAT domain. {ECO
Subcellular LocationCytoplasm {ECO
Tissue SpecificityExpressed in B-cells, hair follicles, foreskin keratinocytes and adult skin. Also expressed in psoriatic tissue.
Web ResourceName=Protein Spotlight; Note=about water - Issue 153 of September 2013; URL="http://web.expasy.org/spotlight/back_issues/153/";