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LMP009983

UniProt Annotations

Entry Information

Gene Name	calcineurin B-like protein 9
Protein Entry	CNBL9_ARATH
UniProt ID	Q9LTB8
Species	Arabidopsis

Comments

Comment type	Description
Disruption Phenotype	Hypersensitivity to abscisic acid in the early developmental stages. {ECO:0000269\|PubMed:15208400}.
Domain	The N-terminal 12 amino acids are sufficient for cell membrane targeting. {ECO:0000269\|PubMed:19832944}.
Function	Acts as a calcium sensor involved in abscisic acid (ABA) signaling and stress-induced ABA biosynthesis pathways. Contributes to the regulation of early stress-related CBF/DREB transcription factors. CBL proteins interact with CIPK serine- threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. May function as a negative regulator of stress and ABA responses. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Involved in the calcium-dependent regulation by CIPK26 of reactive oxygen species production by the NADPH oxidase RBOHF. The CBL9/CIPK3 complex acts in the regulation of abscisic acid response in seed germination. {ECO:0000269\|PubMed:15208400, ECO:0000269\|PubMed:16814720, ECO:0000269\|PubMed:17898163, ECO:0000269\|PubMed:17922773, ECO:0000269\|PubMed:19825536, ECO:0000269\|PubMed:23335733}.
Induction	By drought, cold, salt and abscisic acid (ABA) treatments. {ECO:0000269\|PubMed:15208400}.
Interaction	Q93VD3:CIPK23; NbExp=6; IntAct=EBI-637381, EBI-974277;
Ptm	Both N-myristoylation and calcium-mediated conformational changes are essential for its function. {ECO:0000250}.
Similarity	Belongs to the calcineurin regulatory subunit family. {ECO:0000305}.
Similarity	Contains 4 EF-hand domains. {ECO:0000255\|PROSITE- ProRule:PRU00448}.
Subcellular Location	Cell membrane {ECO:0000269\|PubMed:16814720, ECO:0000269\|PubMed:17922773, ECO:0000269\|PubMed:19832944, ECO:0000269\|PubMed:23335733, ECO:0000269\|PubMed:23482856}; Lipid- anchor {ECO:0000269\|PubMed:16814720, ECO:0000269\|PubMed:17922773, ECO:0000269\|PubMed:19832944, ECO:0000269\|PubMed:23335733, ECO:0000269\|PubMed:23482856}. Note=Plasma membrane localization abolished by 2-bromopalmitate (2-BP) treatment.
Subunit	Homodimer (By similarity). Part of a K(+)-channel calcium-sensing kinase/phosphatase complex composed by a calcium sensor CBL (CBL1, CBL2, CBL3 or CBL9), a kinase CIPK (CIPK6, CIPK16 or CIPK23), a phosphatase PP2C (AIP1) and a K(+)-channel (AKT1). Interacts with CIPK1, CIPK3, CIPK6, CIPK8, CIPK16, CIPK18, CIPK21, CIPK23, CIPK24 and CIPK26. {ECO:0000250, ECO:0000269\|PubMed:14730064, ECO:0000269\|PubMed:16814720, ECO:0000269\|PubMed:17898163, ECO:0000269\|PubMed:17922773, ECO:0000269\|PubMed:19825536, ECO:0000269\|PubMed:21685179, ECO:0000269\|PubMed:23335733}.
Tissue Specificity	Ubiquitous. Colocalized with CIPK23 in root tips and vascular bundles in the stem and the leaf, as well as in guard cells and root hairs. {ECO:0000269\|PubMed:15208400, ECO:0000269\|PubMed:16814720, ECO:0000269\|PubMed:17922773}.