LMPD Database

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LMP003356

UniProt Annotations

Entry Information
Gene Nameglutathione peroxidase 1
Protein EntryGPX1_MOUSE
UniProt IDP11352
SpeciesMouse
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=14 uM for H(2)O(2) (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0) {ECO:0000269|PubMed:21420488}; KM=29 uM for tert-butylperoxide (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0) {ECO:0000269|PubMed:21420488}; Vmax=319 mM/min/mg enzyme toward H(2)O(2) (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0) {ECO:0000269|PubMed:21420488}; Vmax=182 mM/min/mg enzyme toward tert-butylperoxide (at 25 degrees Celsius, in 0.1 M phosphate buffer, pH 7.0) {ECO:0000269|PubMed:21420488};
Catalytic Activity2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O.
CautionPubMed:2771650 sequence was originally thought to originate from human. {ECO:0000305}.
FunctionProtects the hemoglobin in erythrocytes from oxidative breakdown.
MiscellaneousIn the absence of Sod1, Gpx1 in the liver undergoes a 40% reduction in catalytic activity as a result of the decomposition of Sec-47 to dehydroalanine. {ECO:0000305|PubMed:21420488}.
PtmDuring periods of oxidative stress, Sec-47 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine. {ECO:0000269|PubMed:21420488}.
Sequence CautionSequence=CAB43535.1; Type=Miscellaneous discrepancy; Note=Number of sequencing artifacts.; Evidence={ECO:0000305};
SimilarityBelongs to the glutathione peroxidase family. {ECO:0000305}.
Subcellular LocationCytoplasm.
SubunitHomotetramer. Interacts with MIEN1. {ECO:0000269|PubMed:17503775}.