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LIPID Metabolites And Pathways Strategy

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Protocols

Protocol IDs are assigned by LIPID MAPS Laboratory Information Management (LIMS) software. The format of the IDs is as follows:
  • Procedure protocols begin with the letters "PP." Solution protocols begin with the letters "PS."
  • The last 2 numbers of a protocol ID indicate the version number. "00" indicates the initial version. Subsequent revisions of the protocol possess IDs that end with "01", "02", and so on.
  • The version with the largest set of ending numbers is the currently accepted protocol. Previous versions are considered obsolete.
Any core can write a protocol. Except for core D, the title of the new protocol should include the core.
Core D has responsibility for composing consortium-wide protocols involving preparation, handling, and treatment of cells and tissues. Other cores can write protocols that provide additional detail in these areas for their own guidance, provided:
  • The protocols do not contradict or conflict with Core D protocols.
  • The protocols reference pertinent Core D protocols.
  • The protocol title includes the core.

Equipment/supplies

Recommended EquipmentEquipment 
Tissue Culture SuppliesSupplies 
RAW 264.7 Tissue Culture SuppliesPP0000001600Added 08/27/07
RAW 264.7 Cells Recommended EquipmentPP0000001700Added 08/27/07
 

Procedure Protocols

General

Thawing and Passage ProcedurePP0000000101 
Thawing and passage procedure for RAW 264.7 cellsPP0000000100Added 08/27/07
Use of AnimalsPP0000001100Added 08/27/07
Ordering MicePP0000001200Added 08/27/07
Ordering Mice for Foam CellsPP0000002900Added 08/22/06
LIPID MAPS DNA AssayPP0000002700 
Harvesting and Plating Thioglycollate Elicited Macrophages for LM DistributionPP0000004101Added 05/21/08
 

General Kdo2-lipid A Treatment protocols

LPS Induction (Serum-deprived)PP0000000600 
10% Serum Kdo2-lipid A TreatmentPP0000001000Added 08/27/07
10% Serum Kdo2-lipid A TreatmentPP0000001001 
10% Serum Kdo2-lipid A TreatmentPP0000001002 
10% Serum Kdo2-lipid A TreatmentPP0000001003 
10% Serum Kdo2-lipid A TreatmentPP0000001004 
0.5% Serum Kdo2-lipid A TreatmentPP0000000500Added 08/27/07
0.5% Serum Kdo2-lipid A TreatmentPP0000000501Added 08/27/07
 

Primary Macrophages

Eliciting Bio-Gel Derived MacrophagesPP0000001300Added 08/27/07
Eliciting Foam CellsPP0000003000Added 08/22/06
Eliciting Thioglycollate Derived MacrophagesPP0000001400Added 08/27/07
Eliciting Thioglycollate derived MacrophagesPP0000001401Added 06/20/07
Harvesting and Plating Bone Marrow-derived MacrophagesPP0000003500Added 06/20/07
Harvesting and Plating Primary MacrophagesPP0000001501 
Harvesting and Plating Thioglycollate Elicited MacrophagesPP0000001502Added 06/20/07
Harvesting Foam CellsPP0000003100Added 08/22/06
Harvesting MacrophagesPP0000001500Added 08/27/07
Kdo2-lipid A Treatment of Thioglycollate and Bone Marrow Derived MacrophagesPP0000001801Added 06/20/07
Primary Macrophage TreatmentPP0000001800 
 

Core G - Eicosanoids

Core G - Eicosanoid MSPP0000000200Added 08/27/07
Core G - Eicosanoid MSPP0000000201Added 08/27/07
Core G - Eicosanoid MSPP0000000204Added 08/27/07
Core G - Eicosanoid MSPP0000000205Added 08/27/07
Core G - Eicosanoid MSPP0000000206Added 08/27/07
Core G - RAW subcellular fractionationPP0000004300Added 05/19/08
Core G - RAW subcellular fractionationPP0000004301Added 06/10/08
 

Core E - Glycerolipids/Cholesteryl esters

Core E-LM Thawing and Passage for RAW 264.7PP0000001900Added 08/27/07
Core E-Analysis of Neutral LipidsPP0000002200Added 08/27/07
Core E-0.5% Serum Kdo2-lipid A TreatmentPP0000002000Added 08/27/07
Core E-10% Serum Kdo2-lipid A TreatmentPP0000002100Added 08/27/07
 

Core J - Sterols

Core J Compactin Kdo2-lipid A Treatment in Dye-Free GMedPP0000002400Added 08/27/07
Core J Compactin Kdo2-lipid A TreatmentPP0000002800Added 08/27/07
Core J Compactin Kdo2-lipid A ModifiedPP0000003300Added 08/27/07
Core J Sterol Extraction and LCMSPP0000002500Added 08/27/07
Core J 10% NCLPPS, Compactin, Kdo2-lipid A TreatmentPP0000002300Added 08/27/07
Core J Compactin Kdo2lipidA RNAPP0000003400Added 08/27/07
Core J Sterol Extraction and QuantitationPP0000003201Added 08/27/07
Core J Sterol Extraction and QuantitationPP0000003200Added 08/27/07
Core J Foam Cell Sterol Extraction and QuantitationPP0000003600Added 05/21/07
Core J Sterol Extraction, Cleanup, and QuantitationPP0000003700Added 05/21/07
 

Core H - Glycerophospholipids

Core H - Direct Injection MSPP0000000900Added 08/27/07
Core H - HPLC phospholipid protocolPP0000000800Added 08/27/07
Phospholipid LC-MS ParametersPP0000002600Added 08/27/07
Core H - Glycerophospholipid ExtractionPP0000004200Added 04/23/08
 

Solution Protocols

Kdo2-lipid A solutions

Sonicated Kdo2-lipid A Working SolutionPS0000001400 
Sonicated Kdo2-lipid A Working SolutionPS0000001401 
Sonicated Stock Kdo2-lipid APS0000000700Added 08/27/07
Sonicated Stock Kdo2-lipid APS0000000701 
 

Primary Macrophages

Bone Marrow-derived Macrophage Growth MediumPS0000002900Added 06/20/07
Primary Macrophage Growth Medium 1PS0000001701Added 08/22/06
Bio-Gel (2%)PS0000001500Added 08/27/07
Thioglycollate Medium (3%)PS0000001600Added 08/27/07
Primary Macrophage Growth Medium 1PS0000001700Added 08/27/07
Primary Macrophage Serum Deprivation Medium 1PS0000001900Added 08/27/07
 

Cultured Macrophage Cell Lines

RAW 264.7 Growth MediumPS0000000800 
RAW 264.7 Growth Medium 1PS0000000900Added 08/27/07
RAW 264.7 Growth Medium 1PS0000000901Added 08/27/07
RAW 264.7 Growth Medium 1PS0000001100Added 08/27/07
RAW 264.7 Growth Medium 1PS0000001101Added 08/27/07
RAW 264.7 Growth Medium 1PS0000001102Added 08/27/07
RAW 264.7 Growth Medium 1PS0000001200Added 08/27/07
RAW 264.7 Serum Deprivation Medium 1PS0000001000Added 08/27/07
 

Core G - Eicosanoids

Core G Dye-Free Growth MediumPS0000002300Added 08/27/07
Core G Dye-Free Growth MediumPS0000002400Added 08/27/07
Core G - Eicosanoid Primary StandardPS0000000200Added 08/27/07
Core G - Eicosanoid Primary StandardPS0000000601Added 08/27/07
Core G - Eicosanoid Primary StandardPS0000000602Added 08/27/07
Core G - Eicosanoid Primary StandardPS0000000603Added 08/27/07
Core G - Eicosanoid Primary StandardPS0000000604Added 08/27/07
Core G - Eicosanoid Internal StandardPS0000000500Added 08/27/07
Core G - Eicosanoid Internal StandardPS0000000501Added 08/27/07
Core G - Eicosanoid Internal Standards DeuteratedPS0000000101Added 08/27/07
 

Core E - Glycerolipids/Cholesteryl esters

Core E-Neutral Lipid Internal StandardPS0000002000Added 08/27/07
Core E - Cholesterol Ester Internal Standard.PS0000003000Added 08/27/07
 

Core J - Sterols

Core J old 4B OHC D7 standardPS0000002500Added 08/27/07
Core J old 7-Oxo D7standardPS0000002600Added 08/27/07
Core J- old C D7 StandardPS0000002700Added 08/27/07
Core J Mevalonate 20mMPS0000002200Added 08/27/07
Core J Mevalonate 50mMPS0000002800Added 08/27/07
Core J - 10% NCLPPS0000002100Added 08/27/07


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