LMPD Database

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LMP007562

UniProt Annotations

Entry Information
Gene Name4-hydroxy-3-methylbut-2-enyl diphosphate reductase, 4Fe-4S protein
Protein EntryISPH_ECOLI
UniProt IDP62623
SpeciesE. coli
Comments
Comment typeDescription
Biophysicochemical PropertiesKinetic parameters: KM=30 uM for 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate {ECO:0000269|PubMed:15469281}; pH dependence: Optimum pH is 7.0.;
Catalytic ActivityDimethylallyl diphosphate + NAD(P)(+) + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.
Catalytic ActivityIsopentenyl diphosphate + NAD(P)(+) + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H.
CofactorName=[3Fe-4S] cluster; Xref=ChEBI:CHEBI:21137; Evidence={ECO:0000269|PubMed:19569147, ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895}; Note=Binds 1 [3Fe-4S] cluster per subunit. {ECO:0000269|PubMed:19569147, ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895};
Enzyme RegulationAddition of Ca(2+), Mg(2+), Mn(2+), Ni(2+), Co(2+) or Fe(2+) decreases the activity. Addition of Zn(2+) results in complete loss of activity. {ECO:0000269|PubMed:15469281}.
FunctionConverts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Is also involved in penicillin tolerance and control of the stringent response. Seems to directly or indirectly interact with RelA to maintain it in an inactive form during normal growth. {ECO:0000269|PubMed:19569147, ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895}.
InteractionP0A6Y8:dnaK; NbExp=1; IntAct=EBI-554022, EBI-542092;
MiscellaneousPubMed:12706830 and PubMed:22137895 authors have reported the presence of a 4Fe-4S iron-sulfur cluster. It could be due to experimental conditions.
PathwayIsoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3- methylbutenyl diphosphate: step 1/1.
PathwayIsoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1- deoxy-D-xylulose 5-phosphate: step 6/6.
SimilarityBelongs to the IspH family. {ECO:0000305}.
SubunitHomodimer. {ECO:0000269|PubMed:12706830, ECO:0000269|PubMed:19569147, ECO:0000269|PubMed:20080550, ECO:0000269|PubMed:22137895}.