LIPID MAPS

25-hydroxy-cholesterol(d6)

We have 4 spectra for 25-hydroxy-cholesterol(d6). Please select one for more details.

Common Name

LM ID

Instrument

Adduct / Ion

Submitter

25-hydroxy-cholesterol(d6)

LMST01010146

SciEx

[M+NH4]+

25-hydroxy-cholesterol(d6)

LMST01010146

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

25-hydroxy-cholesterol(d6)

LMID

LMST01010146

Systematic name

cholest-5-en-3beta,25-diol(d6)

m/z

391.3841

Adduct / Ion

[M+H-H2O]+

Charge

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

10,20,30

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

6.8

Amount injected

100 nmol

DOI for method

Date acquired

December 2025

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Avanti Research

25-hydroxy-cholesterol(d6)

LMST01010146

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

25-hydroxy-cholesterol(d6)

LMID

LMST01010146

Systematic name

cholest-5-en-3beta,25-diol(d6)

m/z

391.3841

Adduct / Ion

[M+H-H2O]+

Charge

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

20,30,40

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

6.8

Amount injected

100 nmol

DOI for method

Date acquired

December 2025

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Avanti Research

25-hydroxy-cholesterol(d6)

LMST01010146

Thermo Scientific Orbitrap Exploris 240

[M+H-H2O]+

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Structure

Common name

25-hydroxy-cholesterol(d6)

LMID

LMST01010146

Systematic name

cholest-5-en-3beta,25-diol(d6)

m/z

391.3841

Adduct / Ion

[M+H-H2O]+

Charge

Ion source

HESI

Instrument

Thermo Scientific Orbitrap Exploris 240

Resolution

30000

Ion mode

Positive

Collision Energy (CE)

30,40,50

Column

Accucore C30 150 x 2.2

Mobile phase A

Acetonitrile:water 50/50 v/v 5mM AmFm + 0.1% FA

Mobile phase B

Isopropanol:Acetonitrile:water 85/10/5 v/v 5mM AmFm + 0.1% FA

Retention time (min)

6.8

Amount injected

100 nmol

DOI for method

Date acquired

December 2025

Submitters

Fedorova Lab (LMAI) - Gabriele Lombardi Bendoula

Source of standard

Avanti Research