Gene/Proteome Database (LMPD)

LMPD ID
LMP013316
Gene ID
309527
Species
Rattus norvegicus (Rat)
Gene Name
cholesterol 25-hydroxylase
Gene Symbol
Ch25h
Alternate Names
cholesterol 25-hydroxylase; cholesterol 25-monooxygenase;
Chromosome
1
Map Location
1q52
EC Number
1.14.99.38

Proteins

cholesterol 25-hydroxylase
Refseq ID NP_001020586
Protein GI 70794754
UniProt ID Q4QQV7
mRNA ID NM_001025415
Length 298
MACHNVSELQDLGCSNQLLLQPLWDSIRTGEASARSPFFPVIFSIFTYLGFCLPFVVLDVLCPWVPILRRYKIHPDFSPSVRQLLPCLGLTLYQHLVFVFPVTLMHWARSPALLPREAPELSQLLSHVLICLLLFDTEIFAWHLLHHKVPWLYRTFHKVHHQNSSSFALATQYMSVWELLSLTFFDVLNVAMLQCHPLTILVFHVVNIWLSVEDHSGYDFPWSTHRLVPFGWYGGVAHHDLHHSQFNCNFAPYFTHWDKMLGTLRCAPHSKRLCAGSESCLDSGEQCTVHLNQKKKQT

Gene Information

Entrez Gene ID
309527
Gene Name
cholesterol 25-hydroxylase
Gene Symbol
Ch25h
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0001567 IEA:UniProtKB-EC F cholesterol 25-hydroxylase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:0008203 IEA:Ensembl P cholesterol metabolic process
GO:0006633 IEA:InterPro P fatty acid biosynthetic process
GO:0016126 IEA:UniProtKB-KW P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ko00120 Primary bile acid biosynthesis
rno00120 Primary bile acid biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5953720 Bile acid and bile salt metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953930 Synthesis of bile acids and bile salts

Domain Information

InterPro Annotations

Accession Description
IPR006694 Fatty_acid_hydroxylase

UniProt Annotations

Entry Information

Gene Name
cholesterol 25-hydroxylase
Protein Entry
CH25H_RAT
UniProt ID
Q4QQV7
Species
Rat

Comments

Comment Type Description
Catalytic Activity Cholesterol + AH(2) + O(2) = 25- hydroxycholesterol + A + H(2)O.
Cofactor Name=Fe cation; Xref=ChEBI:CHEBI:24875; Evidence= ;
Function Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing (By similarity). In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation
Induction Down-regulated by testosterone
Ptm N-glycosylated
Similarity Belongs to the sterol desaturase family
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein .
Tissue Specificity Expressed in testicular macrophages at all stages, with the highest level in 10 day old animals

Identical and Related Proteins

Unique RefSeq proteins for LMP013316 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
70794754 RefSeq NP_001020586 298 cholesterol 25-hydroxylase

Identical Sequences to LMP013316 proteins

Reference Database Accession Length Protein Name
GI:70794754 EMBL CAR81281.1 298 unnamed protein product [Rattus norvegicus]
GI:70794754 EMBL CBX84759.1 298 unnamed protein product [Rattus norvegicus]
GI:70794754 GenBank EDM13160.1 298 cholesterol 25-hydroxylase [Rattus norvegicus]
GI:70794754 RefSeq XP_006231353.1 298 PREDICTED: cholesterol 25-hydroxylase isoform X1 [Rattus norvegicus]

Related Sequences to LMP013316 proteins

Reference Database Accession Length Protein Name
GI:70794754 EMBL CAR81281.1 298 unnamed protein product [Rattus norvegicus]
GI:70794754 EMBL CBX84759.1 298 unnamed protein product [Rattus norvegicus]
GI:70794754 GenBank AAH97964.1 298 Cholesterol 25-hydroxylase [Rattus norvegicus]
GI:70794754 GenBank EDM13160.1 298 cholesterol 25-hydroxylase [Rattus norvegicus]