Gene/Proteome Database (LMPD)

LMPD ID
LMP013168
Gene ID
298942
Species
Rattus norvegicus (Rat)
Gene Name
dihydrolipoamide dehydrogenase
Gene Symbol
Dld
Alternate Names
dihydrolipoyl dehydrogenase, mitochondrial; dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex);
Chromosome
6
Map Location
6q16
EC Number
1.8.1.4
Summary
This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. A similar protein in human, mouse, and pig has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease. [provided by RefSeq, Jan 2014]
Orthologs
View orthologs and multiple alignments for Dld

Proteins

dihydrolipoyl dehydrogenase, mitochondrial precursor
Refseq ID NP_955417
Protein GI 40786469
UniProt ID Q6P6R2
mRNA ID NM_199385
Length 509
MQSWSRVYCSLAKKGHFNRLSHGLQGASSVPLRTYSDQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHLAHGKDFASRGIEIPEVRLNLEKMMEQKRSAVKALTGGIAHLFKQNKVVHVNGFGKITGKNQVTATTADGSTQVIGTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKLVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGIGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSVEAASGGKAEVITCDVLLVCIGRRPFTQNLGLEELGIELDPKGRIPVNTRFQTKIPNIFAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGVEFKVGKFPFAANSRAKTNADTDGMVKILGHKSTDRILGAHILGPGAGEMVNEAALALEYGASCEDVARVCHAHPTLSEAFREANLAASFGKPINF
transit_peptide: 1..35 inference: non-experimental evidence, no additional details recorded note: Mitochondrion (By similarity); propagated from UniProtKB/Swiss-Prot (Q6P6R2.1) calculated_mol_wt: 3967 peptide sequence: MQSWSRVYCSLAKKGHFNRLSHGLQGASSVPLRTY mat_peptide: 36..509 product: Dihydrolipoyl dehydrogenase, mitochondrial experiment: experimental evidence, no additional details recorded note: propagated from UniProtKB/Swiss-Prot (Q6P6R2.1) calculated_mol_wt: 50090 peptide sequence: SDQPIDADVTVIGSGPGGYVAAIKAAQLGFKTVCIEKNETLGGTCLNVGCIPSKALLNNSHYYHLAHGKDFASRGIEIPEVRLNLEKMMEQKRSAVKALTGGIAHLFKQNKVVHVNGFGKITGKNQVTATTADGSTQVIGTKNILIATGSEVTPFPGITIDEDTIVSSTGALSLKKVPEKLVVIGAGVIGVELGSVWQRLGADVTAVEFLGHVGGIGIDMEISKNFQRILQKQGFKFKLNTKVTGATKKSDGKIDVSVEAASGGKAEVITCDVLLVCIGRRPFTQNLGLEELGIELDPKGRIPVNTRFQTKIPNIFAIGDVVAGPMLAHKAEDEGIICVEGMAGGAVHIDYNCVPSVIYTHPEVAWVGKSEEQLKEEGVEFKVGKFPFAANSRAKTNADTDGMVKILGHKSTDRILGAHILGPGAGEMVNEAALALEYGASCEDVARVCHAHPTLSEAFREANLAASFGKPINF

Gene Information

Entrez Gene ID
298942
Gene Name
dihydrolipoamide dehydrogenase
Gene Symbol
Dld
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005739 IDA:RGD C mitochondrion
GO:0045252 IDA:RGD C oxoglutarate dehydrogenase complex
GO:0045254 IDA:RGD C pyruvate dehydrogenase complex
GO:0051287 IDA:RGD F NAD binding
GO:0004148 IDA:RGD F dihydrolipoyl dehydrogenase activity
GO:0050660 IDA:RGD F flavin adenine dinucleotide binding
GO:0043544 IDA:RGD F lipoamide binding
GO:0006103 IDA:RGD P 2-oxoglutarate metabolic process
GO:0006086 IDA:RGD P acetyl-CoA biosynthetic process from pyruvate
GO:0007568 IEP:RGD P aging
GO:0045454 IEA:InterPro P cell redox homeostasis
GO:0051068 IDA:RGD P dihydrolipoamide metabolic process
GO:0009106 IDA:RGD P lipoate metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko01200 Carbon metabolism
rno01200 Carbon metabolism
ko00020 Citrate cycle (TCA cycle)
rno00020 Citrate cycle (TCA cycle)
M00009 Citrate cycle (TCA cycle, Krebs cycle)
M00011 Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate
ko00260 Glycine, serine and threonine metabolism
rno00260 Glycine, serine and threonine metabolism
ko00010 Glycolysis / Gluconeogenesis
rno00010 Glycolysis / Gluconeogenesis
M00036 Leucine degradation, leucine => acetoacetate + acetyl-CoA
rno01100 Metabolic pathways
ko00620 Pyruvate metabolism
rno00620 Pyruvate metabolism
M00307 Pyruvate oxidation, pyruvate => acetyl-CoA
ko00280 Valine, leucine and isoleucine degradation
rno00280 Valine, leucine and isoleucine degradation

REACTOME Pathway Links

REACTOME Pathway ID Description
5953282 Branched-chain amino acid catabolism
5953285 Citric acid cycle (TCA cycle)
5953284 Lysine catabolism
5953250 Metabolism
5953272 Metabolism of amino acids and derivatives
5953268 Pyruvate metabolism
5953269 Pyruvate metabolism and Citric Acid (TCA) cycle
5954047 Regulation of pyruvate dehydrogenase (PDH) complex
5953270 The citric acid (TCA) cycle and respiratory electron transport

Domain Information

InterPro Annotations

Accession Description
IPR006258 Dihydrolipoamide dehydrogenase
IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase
IPR016156 FAD/NAD-linked reductase, dimerisation domain
IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain
IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain
IPR012999 Pyridine nucleotide-disulphide oxidoreductase, class I, active site
IPR004099 Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain

UniProt Annotations

Entry Information

Gene Name
dihydrolipoamide dehydrogenase
Protein Entry
DLDH_RAT
UniProt ID
Q6P6R2
Species
Rat

Comments

Comment Type Description
Catalytic Activity Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH.
Cofactor Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence= ; Note=Binds 1 FAD per subunit. ;
Function Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction (By similarity)
Miscellaneous The active site is a redox-active disulfide bond.
Ptm Tyrosine phosphorylated
Similarity Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family
Subcellular Location Mitochondrion matrix .
Subunit Homodimer. Eukaryotic pyruvate dehydrogenase complexes are organized about a core consisting of the oligomeric dihydrolipoamide acetyl-transferase, around which are arranged multiple copies of pyruvate dehydrogenase, dihydrolipoamide dehydrogenase and protein X bound by non-covalent bonds (By similarity)

Identical and Related Proteins

Unique RefSeq proteins for LMP013168 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
40786469 RefSeq NP_955417 509 dihydrolipoyl dehydrogenase, mitochondrial precursor

Identical Sequences to LMP013168 proteins

Reference Database Accession Length Protein Name
GI:40786469 GenBank AAH62069.1 509 Dihydrolipoamide dehydrogenase [Rattus norvegicus]
GI:40786469 GenBank EDM03250.1 509 dihydrolipoamide dehydrogenase, isoform CRA_b [Rattus norvegicus]
GI:40786469 SwissProt Q6P6R2.1 509 RecName: Full=Dihydrolipoyl dehydrogenase, mitochondrial; AltName: Full=Dihydrolipoamide dehydrogenase; Flags: Precursor [Rattus norvegicus]

Related Sequences to LMP013168 proteins

Reference Database Accession Length Protein Name
GI:40786469 GenBank AAH62069.1 509 Dihydrolipoamide dehydrogenase [Rattus norvegicus]
GI:40786469 GenBank EDM03250.1 509 dihydrolipoamide dehydrogenase, isoform CRA_b [Rattus norvegicus]
GI:40786469 RefSeq XP_006989579.1 509 PREDICTED: dihydrolipoyl dehydrogenase, mitochondrial [Peromyscus maniculatus bairdii]
GI:40786469 SwissProt Q6P6R2.1 509 RecName: Full=Dihydrolipoyl dehydrogenase, mitochondrial; AltName: Full=Dihydrolipoamide dehydrogenase; Flags: Precursor [Rattus norvegicus]