Gene/Proteome Database (LMPD)

LMPD ID
LMP012939
Gene ID
171142
Species
Rattus norvegicus (Rat)
Gene Name
enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
Gene Symbol
Ehhadh
Synonyms
1; Lbp; MEF; Mfe; Pbe; Mfe1; Pbfe; perMFE-1;
Alternate Names
peroxisomal bifunctional enzyme; multifunctional enzyme type 1; L-specific bifunctional protein; peroxisomal bifunctional enzyme type 1; enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase;
Chromosome
11
Map Location
11q23
EC Number
4.2.1.17
Summary
enzyme involved in the B-oxidation cycle of peroxisomes [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Ehhadh

Proteins

peroxisomal bifunctional enzyme
Refseq ID NP_598290
Protein GI 19424318
UniProt ID P07896
mRNA ID NM_133606
Length 722
MAEYLRLPHSLAMIRLCNPPVNAVSPTVIREVRNGLQKAGSDHTVKAIVICGANGNFCAGADIHGFSAFTPGLALGSLVDEIQRYQKPVLAAIQGVALGGGLELALGCHYRIANAKARVGLPEVTLGILPGARGTQLLPRVVGVPVALDLITSGKYLSADEALRLGILDAVVKSDPVEEAIKFAQKIIDKPIEPRRIFNKPVPSLPNMDSVFAEAIAKVRKQYPGVLAPETCVRSIQASVKHPYEVGIKEEEKLFMYLRASGQAKALQYAFFAEKSANKWSTPSGASWKTASAQPVSSVGVLGLGTMGRGIAISFARVGISVVAVESDPKQLDAAKKIITFTLEKEASRAHQNGQASAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALNVDDIASSTDRPQLVIGTHFFSPAHVMRLLEVIPSRYSSPTTIATVMSLSKKIGKIGVVVGNCYGFVGNRMLAPYYNQGFFLLEEGSKPEDVDGVLEEFGFKMGPFRVSDLAGLDVGWKIRKGQGLTGPSLPPGTPVRKRGNSRYSPLGDMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLSTFLSQYREVHHIEQRTISKEEILERCLYSLINEAFRILEEGMAARPEHIDVIYLHGYGWPRHKGGPMFYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYLRRLVAQGSPPLKEWQSLAGPHGSKL

Gene Information

Entrez Gene ID
171142
Gene Name
enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
Gene Symbol
Ehhadh
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:UniProtKB C cytosol
GO:0005739 IEA:Ensembl C mitochondrion
GO:0005634 IEA:Ensembl C nucleus
GO:0005777 IDA:UniProtKB C peroxisome
GO:0003857 IDA:RGD F 3-hydroxyacyl-CoA dehydrogenase activity
GO:0050662 IEA:InterPro F coenzyme binding
GO:0004165 IEA:UniProtKB-EC F dodecenoyl-CoA delta-isomerase activity
GO:0004300 IEA:UniProtKB-EC F enoyl-CoA hydratase activity
GO:0019899 IPI:UniProtKB F enzyme binding
GO:0006635 IEA:UniProtKB-UniPathway P fatty acid beta-oxidation
GO:0006475 ISS:UniProtKB P internal protein amino acid acetylation

KEGG Pathway Links

KEGG Pathway ID Description
ko00650 Butanoate metabolism
rno00650 Butanoate metabolism
ko01200 Carbon metabolism
rno01200 Carbon metabolism
ko00071 Fatty acid degradation
rno00071 Fatty acid degradation
ko01212 Fatty acid metabolism
rno01212 Fatty acid metabolism
ko00310 Lysine degradation
rno00310 Lysine degradation
M00032 Lysine degradation, lysine => saccharopine => acetoacetyl-CoA
M00013 Malonate semialdehyde pathway, propanoyl-CoA => Acetyl-CoA
rno01100 Metabolic pathways
ko03320 PPAR signaling pathway
rno03320 PPAR signaling pathway
ko04146 Peroxisome
rno04146 Peroxisome
ko00640 Propanoate metabolism
rno00640 Propanoate metabolism
ko00380 Tryptophan metabolism
rno00380 Tryptophan metabolism
ko00280 Valine, leucine and isoleucine degradation
rno00280 Valine, leucine and isoleucine degradation
ko00410 beta-Alanine metabolism
rno00410 beta-Alanine metabolism
M00087 beta-Oxidation

Domain Information

InterPro Annotations

Accession Description
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding
IPR006180 3-hydroxyacyl-CoA dehydrogenase, conserved site
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like
IPR029045 ClpP/crotonase-like domain
IPR001753 Crotonase_core_superfam
IPR013328 Dehydrogenase, multihelical
IPR018376 Enoyl-CoA hydratase/isomerase, conserved site
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
enoyl-CoA, hydratase/3-hydroxyacyl CoA dehydrogenase
Protein Entry
ECHP_RAT
UniProt ID
P07896
Species
Rat

Comments

Comment Type Description
Catalytic Activity (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O.
Catalytic Activity (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl- CoA.
Catalytic Activity (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.
Enzyme Regulation Enzyme activity enhanced by acetylation
Pathway Lipid metabolism; fatty acid beta-oxidation.
Ptm Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TCA) or with nicotinamide (NAM) with highest increase on Lys-345. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids (By similarity)
Similarity In the C-terminal section; belongs to the 3- hydroxyacyl-CoA dehydrogenase family
Similarity In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family
Subcellular Location Peroxisome.
Subunit Monomer

Identical and Related Proteins

Unique RefSeq proteins for LMP012939 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
19424318 RefSeq NP_598290 722 peroxisomal bifunctional enzyme

Identical Sequences to LMP012939 proteins

Reference Database Accession Length Protein Name
GI:19424318 EMBL CBG04557.1 722 unnamed protein product [Rattus norvegicus]
GI:19424318 EMBL CBV18079.1 722 unnamed protein product [Rattus norvegicus]
GI:19424318 GenBank EDL78038.1 722 enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase [Rattus norvegicus]
GI:19424318 GenBank AGX95964.1 722 Sequence 72188 from patent US 8541208

Related Sequences to LMP012939 proteins

Reference Database Accession Length Protein Name
GI:19424318 GenBank AAA41825.1 722 peroxisomal enoyl-CoA: hydrotase-3-hydroxyacyl-CoA [Rattus norvegicus]
GI:19424318 GenBank AAH89777.1 722 Enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase [Rattus norvegicus]
GI:19424318 GenBank AGX95964.1 722 Sequence 72188 from patent US 8541208
GI:19424318 SwissProt P07896.2 722 RecName: Full=Peroxisomal bifunctional enzyme; Short=PBE; Short=PBFE; Includes: RecName: Full=Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; Includes: RecName: Full=3-hydroxyacyl-CoA dehydrogenase [Rattus norvegicus]