LIPID MAPS

LMPD ID

LMP012842

Gene ID

94338

Species

Rattus norvegicus (Rat)

Gene Name

sphingomyelin phosphodiesterase 3, neutral membrane

Gene Symbol

Smpd3

Synonyms

cca1;

Alternate Names

sphingomyelin phosphodiesterase 3; nSMase2; nSMase-2; neutral sphingomyelinase 2; neutral sphingomyelinase II; confluent 3Y1 cell-associated 1; confluent 3Y1 cell-associated protein 1; neutral sphingomyelin phosphodiesterase 3;

Chromosome

19

Map Location

19q12

EC Number

3.1.4.12

Summary

mRNA accumulation occurs in a growth arrested confluent monolayer but not in subconfluent cells; may play a role in regulation of cell growth [RGD, Feb 2006]

Orthologs

View orthologs and multiple alignments for Smpd3

sphingomyelin phosphodiesterase 3
Refseq ID	NP_446057
Protein GI	16758394
UniProt ID	O35049
mRNA ID	NM_053605
Length	635
MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLVASFIPTTYEKRQRADDPCYLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLEDKSPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRNGAARPQIKIYIDSPTNTSISAASFSSLVSPQGSDGARAVPGSIKRTASVEYKGDGGRHPSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRAQEADDPAPGSQARNGAGGTPKGQTPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGSNSKLLYKTSVVKKAAARRRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGCHGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQRIVGYIACTHLHAPPEDSAIRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGDLNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDINGLYDEDVCTPDNLQKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGCALTGRLRWKNSVLSPSCPA

Entrez Gene ID

94338

Gene Name

sphingomyelin phosphodiesterase 3, neutral membrane

Gene Symbol

Smpd3

Species

Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005794	IEA:UniProtKB-KW	C	Golgi apparatus
GO:0005886	IEA:UniProtKB-KW	C	plasma membrane
GO:0046872	IEA:UniProtKB-KW	F	metal ion binding
GO:0004767	IEA:UniProtKB-EC	F	sphingomyelin phosphodiesterase activity
GO:0007049	IEA:UniProtKB-KW	P	cell cycle
GO:0007275	IEA:UniProtKB-KW	P	multicellular organismal development
GO:0006665	IEA:UniProtKB-UniPathway	P	sphingolipid metabolic process

KEGG Pathway Links

KEGG Pathway ID	Description
rno01100	Metabolic pathways
ko00600	Sphingolipid metabolism
rno00600	Sphingolipid metabolism

REACTOME Pathway Links

REACTOME Pathway ID	Description
5954485	Glycosphingolipid metabolism
5953250	Metabolism
5953289	Metabolism of lipids and lipoproteins
5954267	Sphingolipid metabolism

InterPro Annotations

Accession	Description
IPR005135	Endonuclease/exonuclease/phosphatase

Entry Information

Gene Name

sphingomyelin phosphodiesterase 3, neutral membrane

Protein Entry

NSMA2_RAT

UniProt ID

O35049

Species

Rat

Comments

Comment Type	Description
Catalytic Activity	Sphingomyelin + H(2)O = N-acylsphingosine + phosphocholine
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence= ;
Function	Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Acts as a regulator of postnatal development and participates in bone and dentin mineralization. Overexpression enhances cell death, suggesting that it may be involved in apoptosis control. May be involved in IL-1-beta-induced JNK activation in hepatocytes. May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF- kappa-B activation under inflammatory conditions
Pathway	Lipid metabolism; sphingolipid metabolism.
Ptm	Palmitoylated, palmitoylation-deficient proteins are targeted for lysosomal degradation
Sequence Caution	Sequence=BAA22932.1; Type=Frameshift; Positions=616; Evidence= ;
Similarity	Belongs to the neutral sphingomyelinase family
Subcellular Location	Golgi apparatus membrane; Lipid-anchor. Cell membrane {ECO:0000250}; Lipid-anchor . Note=May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons
Tissue Specificity	In brain sections, it is restricted to neurons and especially prominent in large cells, including Purkinje cells, pyramidal cells, neurons of the dentate gyrus granular layer, and neurons in the pontine nuclei. Also present in the hypothalamic nuclei, neurons in the piriform cortex, and nuclei of the brainstem (at protein level). Mainly expressed in brain and jejunum. Weakly or not expressed in heart, spleen, lung, liver, kidney and testis

Unique RefSeq proteins for LMP012842 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
16758394	RefSeq	NP_446057	635	sphingomyelin phosphodiesterase 3

Identical Sequences to LMP012842 proteins

Reference	Database	Accession	Length	Protein Name
GI:16758394	DBBJ	BAA22932.1	635	CCA1 protein [Rattus norvegicus]

Related Sequences to LMP012842 proteins

Reference	Database	Accession	Length	Protein Name
GI:16758394	DBBJ	BAA22932.1	635	CCA1 protein [Rattus norvegicus]
GI:16758394	RefSeq	XP_008770763.1	655	PREDICTED: sphingomyelin phosphodiesterase 3 isoform X1 [Rattus norvegicus]
GI:16758394	SwissProt	O35049.2	655	RecName: Full=Sphingomyelin phosphodiesterase 3; AltName: Full=Confluent 3Y1 cell-associated protein 1; AltName: Full=Neutral sphingomyelinase 2; Short=nSMase-2; Short=nSMase2; AltName: Full=Neutral sphingomyelinase II [Rattus norvegicus]