LIPID MAPS

LMPD ID

LMP012686

Gene ID

64019

Species

Rattus norvegicus (Rat)

Gene Name

phosphoprotein membrane anchor with glycosphingolipid microdomains 1

Gene Symbol

Pag1

Synonyms

Cbp;

Alternate Names

phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Csk binding protein; csk-binding protein; transmembrane phosphoprotein Cbp; phosphoprotein associated with glycosphingolipid microdomains 1;

Chromosome

2

Map Location

chromosome:2

Summary

plays a role in negative regulation of FcepsilonRI signaling [RGD, Feb 2006]

Orthologs

View orthologs and multiple alignments for Pag1

phosphoprotein associated with glycosphingolipid-enriched microdomains 1
Refseq ID	NP_071589
Protein GI	11560063
UniProt ID	Q9JM80
mRNA ID	NM_022253
Length	424
MGPAGSALSSGQMQMQMVLWGSLAAVAMFFLITFLILLCSSCDRDKKPRQHSGDHESLMNVPSDKEMFSHSATSLTTDALASSEQNGVLTNGDILSEDSTMTCMQHYEEVQTSASDLLDSQDSTGKAKCHQSRELPRIPPENAVDAMLTARAADGDSGPGVEGPYEVLKDSSSQENMVEDCLYETVKEIKEVADKSQGGKSKSTSALKELQGAHAEGKADFAEYASVDRNKKCRHSTNAESILGTSSDLDEETPPPVPVKLLDENANLPEKGEHGAEEQAPEAPSGHSKRFSSLSYKSREEDPTLTEEEISAMYSSVNKPGQSAHKPGPESTCQCPQGPPQRSSSSCNDLYATVKDFEKTPNSISMLPPARRPGEEPEPDYEAIQTLNREDDKVPLGTNGHLVPKENDYESIGDLQQGRDVTRL

Entrez Gene ID

64019

Gene Name

phosphoprotein membrane anchor with glycosphingolipid microdomains 1

Gene Symbol

Pag1

Species

Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005887	TAS:RGD	C	integral component of plasma membrane
GO:0005068	TAS:RGD	F	transmembrane receptor protein tyrosine kinase adaptor activity
GO:0002376	IEA:UniProtKB-KW	P	immune system process
GO:0007165	TAS:RGD	P	signal transduction
GO:0007169	TAS:GOC	P	transmembrane receptor protein tyrosine kinase signaling pathway

InterPro Annotations

Accession	Description

Entry Information

Gene Name

phosphoprotein membrane anchor with glycosphingolipid microdomains 1

Protein Entry

PHAG1_RAT

UniProt ID

Q9JM80

Species

Rat

Comments

Comment Type	Description
Caution	In contrast to the human ortholog, does not seem to interact with FYN
Caution	In contrast to the human ortholog, does not seem to interact with FYN. {ECO:0000305\|PubMed:10801129}.
Function	Negatively regulates TCR (T-cell antigen receptor)- mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. {ECO:0000269\|PubMed:10801129, ECO:0000269\|PubMed:10918051, ECO:0000269\|PubMed:11859092}.
Ptm	Palmitoylated
Ptm	Palmitoylated. {ECO:0000250}.
Ptm	Phosphorylated by FYN on Tyr-314 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN in response to EPO. {ECO:0000269\|PubMed:10801129, ECO:0000269\|PubMed:10918051, ECO:0000269\|PubMed:11859092, ECO:0000269\|PubMed:14613929, ECO:0000269\|PubMed:16920712}.
Subcellular Location	Cell membrane {ECO:0000269\|PubMed:10801129, ECO:0000269\|PubMed:11859092}; Single-pass type III membrane protein {ECO:0000269\|PubMed:10801129, ECO:0000269\|PubMed:11859092}. Note=Present in lipid rafts.
Subunit	Interacts with SLC9A3R1/EBP50. In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation (By similarity). When phosphorylated, interacts with CSK. Identified in a complex with LYN and STAT3 (By similarity). Interacts with LYN. {ECO:0000250, ECO:0000269\|PubMed:10801129, ECO:0000269\|PubMed:11859092, ECO:0000269\|PubMed:14613929, ECO:0000269\|PubMed:16920712}.
Tissue Specificity	Ubiquitously expressed, with highest levels in developing brain, lung, thymus, spleen and testis. Present in mast cells
Tissue Specificity	Ubiquitously expressed, with highest levels in developing brain, lung, thymus, spleen and testis. Present in mast cells. {ECO:0000269\|PubMed:10801129, ECO:0000269\|PubMed:11859092}.

Unique RefSeq proteins for LMP012686 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
11560063	RefSeq	NP_071589	424	phosphoprotein associated with glycosphingolipid-enriched microdomains 1

Identical Sequences to LMP012686 proteins

Reference	Database	Accession	Length	Protein Name
GI:11560063	DBBJ	BAA95413.1	424	Csk binding protein Cbp [Rattus norvegicus]
GI:11560063	SwissProt	Q9JM80.1	424	RecName: Full=Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; AltName: Full=Csk-binding protein; AltName: Full=Transmembrane phosphoprotein Cbp [Rattus norvegicus]

Related Sequences to LMP012686 proteins

Reference	Database	Accession	Length	Protein Name
GI:11560063	DBBJ	BAA95413.1	424	Csk binding protein Cbp [Rattus norvegicus]
GI:11560063	RefSeq	NP_444412.2	429	phosphoprotein associated with glycosphingolipid-enriched microdomains 1 [Mus musculus]
GI:11560063	SwissProt	Q9JM80.1	424	RecName: Full=Phosphoprotein associated with glycosphingolipid-enriched microdomains 1; AltName: Full=Csk-binding protein; AltName: Full=Transmembrane phosphoprotein Cbp [Rattus norvegicus]