Gene/Proteome Database (LMPD)

LMPD ID
LMP012648
Gene ID
54259
Species
Rattus norvegicus (Rat)
Gene Name
inositol polyphosphate-5-phosphatase D
Gene Symbol
Inpp5d
Alternate Names
phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; SHIP-1; Inositol polyphosphate-5-phosphatase 145 kDa; SH2 domain-containing inositol phosphatase 1; Inositol polyphosphate-5-phosphatase, 145 kDa; SH2 domain-containing inositol 5'-phosphatase 1; SH2 domain-containing inositol-5'-phosphatase 1; phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1;
Chromosome
9
Map Location
9q35
EC Number
3.1.3.86
Summary
phosphatase involved in the inhibitory action of the mast cell function-associated antigen [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Inpp5d

Proteins

phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Refseq ID NP_062184
Protein GI 9506813
UniProt ID P97573
mRNA ID NM_019311
Length 1190
MPAMVPGWNHGNITRSKAEELLSRAGKDGSFLVRASESIPRAYALCVLFRNCVYTYRILPNEDDKFTVQASEGVPMRFFTKLDQLIEFYKKENMGLVTHLQFPVPLEEEDAIDEPEEDTESVMSPPELPPRNIPVSGGPCEAKDLPLPTENPRAPEVTRLSLSETLFQRLQSMDTSGLPEEHLKAIQDYLSTQLMLDSDFLKTGSSNLPHLKKLTSLLCKELHGEVIRTLPSLESLQRLFDQQLSPGLRPRPQVPGEANPITMVAKLSQLTSLLSSIEDKVKALLHEGSESTNRRSLIPPVTFEVKSESLGIPQKMHLKVDVESGKLIIKKSRDGSEDKFYSHKKILQLIKSQKFLNKLVILVETEKEKILRKEYVFSDSKKREGFCQLLQQMKNKHSEQSEPDMITIFIGTWNMGNAPPPKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLEILRHSLQEVTSMTFKTVAIHTLWNIRIVVLAKPEHENRISHICTDNVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYMNILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRVELPTWEAEAIIQKIKQQQYSDLLAHDQLLLERKEQEVFLHFEEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMTSDHSPVFATFEAGVTSQFVSKNGPGAVDSQGQIEFLACYATLKTKSQTKFYLELHSSCLESFVKSQEGENEEGDEGELVVRFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLETTESQLPIYTPLTHHGEMTGHFRGEIKLQTSEGKMREKLYDFVKTERDESSGMKCLKNLTSHDPMRQWEPAGRVPACGISSLNEIINPNYIGMGPFGQPLHGKSTLSPDQQLTAWSYDQLPKDSSLGPGRGEGPPTPPSQPPLSPKKFSSSTANRGSCPRVQETRPGDLGKVEALPQEDLPLTKPEMFENPLYGSVSPFPKLVPRKEQESPKMMRKEPPPCPDPGVSSPSIMLPKAQEVENVKGTSKQAPVPVFGPTPRIRSFTCSSSAEGRMPSGDKSQGKPKAPASSQAPVPVKRPVKPSRSEMSQQTTPIPAPRPPLPVKSPAVLQLQHSKGRDYRDNTELPHHGKHRQEESLLGRTAMQ

Gene Information

Entrez Gene ID
54259
Gene Name
inositol polyphosphate-5-phosphatase D
Gene Symbol
Inpp5d
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005884 IDA:RGD C actin filament
GO:0030863 IDA:RGD C cortical cytoskeleton
GO:0005829 IDA:RGD C cytosol
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0030487 IDA:RGD F inositol-4,5-bisphosphate 5-phosphatase activity
GO:0006915 IEA:UniProtKB-KW P apoptotic process
GO:0002376 IEA:UniProtKB-KW P immune system process
GO:0046856 IEA:InterPro P phosphatidylinositol dephosphorylation

KEGG Pathway Links

KEGG Pathway ID Description
ko04662 B cell receptor signaling pathway
rno04662 B cell receptor signaling pathway
ko04664 Fc epsilon RI signaling pathway
rno04664 Fc epsilon RI signaling pathway
ko04666 Fc gamma R-mediated phagocytosis
rno04666 Fc gamma R-mediated phagocytosis
ko00562 Inositol phosphate metabolism
rno00562 Inositol phosphate metabolism
ko04070 Phosphatidylinositol signaling system
rno04070 Phosphatidylinositol signaling system

Domain Information

InterPro Annotations

Accession Description
IPR005135 Endonuclease/exonuclease/phosphatase
IPR000300 Inositol polyphosphate-related phosphatase
IPR000980 SH2 domain

UniProt Annotations

Entry Information

Gene Name
inositol polyphosphate-5-phosphatase D
Protein Entry
SHIP1_RAT
UniProt ID
P97573
Species
Rat

Comments

Comment Type Description
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 3,4,5- triphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 3,4- diphosphate + phosphate
Domain The NPXY sequence motif found in many tyrosine- phosphorylated proteins is required for the specific binding of the PID domain
Domain The SH2 domain interacts with tyrosine phosphorylated forms of proteins such as SHC1 or PTPN11/SHP-2. It competes with that of GRB2 for binding to phosphorylated SHC1 to inhibit the Ras pathway. It is also required for tyrosine phosphorylation (By similarity)
Enzyme Regulation Activated upon translocation to the sites of synthesis of PtdIns(3,4,5)P3 in the membrane
Function Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol- 3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6 (By similarity)
Ptm Tyrosine phosphorylated by the members of the SRC family after exposure to a diverse array of extracellular stimuli such as cytokines, growth factors, antibodies, chemokines, integrin ligands and hypertonic and oxidative stress. Phosphorylated upon IgG receptor FCGR2B-binding
Similarity Belongs to the inositol 1,4,5-trisphosphate 5- phosphatase family
Similarity Contains 1 SH2 domain. {ECO:0000255|PROSITE- ProRule:PRU00191}.
Subcellular Location Cytoplasm . Membrane {ECO:0000250}; Peripheral membrane protein . Note=Translocates to the plasma membrane when activated, translocation is probably due to different mechanisms depending on the stimulus and cell type. Partly translocated via its SH2 domain which mediates interaction with tyrosine phosphorylated receptors such as the FC-gamma-RIIB receptor (FCGR2B) or CD16/FCGR3. Tyrosine phosphorylation may also participate in membrane localization (By similarity)
Subunit Interacts with tyrosine phosphorylated forms of SHC1, DOK1, DOK3, PTPN11/SHP-2, SLAMF1/CD150. Interacts with PTPN11 in response to IL-3. Interacts with receptors EPOR, MS4A2/FCER1B and FCER1G, FCGR2A, FCGR2B and FCGR3. Interacts with GRB2 and PLCG1. Interacts with tyrosine kinases SRC and TEC. Interacts with FCGR2A, leading to regulate gene expression during the phagocytic process. Interacts with c-Met/MET (By similarity). Interacts with MILR1 (tyrosine-phosphorylated). Can weakly interact (via NPXY motif 2) with DAB2 (via PID domain); the interaction is impaired by tyrosine phosphorylation of the NPXY motif. {ECO:0000250, ECO:0000269|PubMed:8910587}.

Identical and Related Proteins

Unique RefSeq proteins for LMP012648 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
9506813 RefSeq NP_062184 1190 phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1

Identical Sequences to LMP012648 proteins

Reference Database Accession Length Protein Name
GI:9506813 GenBank AAB40610.1 1190 inositol polyphosphate 5' phosphatase Ship [Rattus norvegicus]
GI:9506813 SwissProt P97573.1 1190 RecName: Full=Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; AltName: Full=SH2 domain-containing inositol 5'-phosphatase 1; Short=SH2 domain-containing inositol phosphatase 1; Short=SHIP-1 [Rattus norvegicus]

Related Sequences to LMP012648 proteins

Reference Database Accession Length Protein Name
GI:9506813 GenBank AAB40610.1 1190 inositol polyphosphate 5' phosphatase Ship [Rattus norvegicus]
GI:9506813 GenBank AAI08329.1 1190 Inositol polyphosphate-5-phosphatase D [Mus musculus]
GI:9506813 SwissProt P97573.1 1190 RecName: Full=Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1; AltName: Full=SH2 domain-containing inositol 5'-phosphatase 1; Short=SH2 domain-containing inositol phosphatase 1; Short=SHIP-1 [Rattus norvegicus]