LIPID MAPS

LMPD ID

LMP012613

Gene ID

29580

Species

Rattus norvegicus (Rat)

Gene Name

farnesyl diphosphate farnesyl transferase 1

Gene Symbol

Fdft1

Alternate Names

squalene synthase; SS; SQS; squalene synthetase; FPP:FPP farnesyltransferase; farnesyl diphosphate farnesyltransferase; farnesyl-diphosphate farnesyltransferase;

Chromosome

15

Map Location

15p12

EC Number

2.5.1.21

Summary

catalyzes the conversion of trans-farnesyl diphosphate to squalene; plays a role in biosynthesis of cholesterol and of isoprenoids [RGD, Feb 2006]

Orthologs

View orthologs and multiple alignments for Fdft1

squalene synthase
Refseq ID	NP_062111
Protein GI	9506591
UniProt ID	Q02769
mRNA ID	NM_019238
Length	416
MEFVKCLGHPEEFYNLLRFRMGGRRNFIPKMDRNSLSNSLKTCYKYLDQTSRSFAAVIQALDGDIRHAVCVFYLILRAMDTVEDDMAISVEKKIPLLRNFHTFLYEPEWRFTESKEKHRVVLEDFPTISLEFRNLAEKYQTVIADICHRMGCGMAEFLNKDVTSKQDWDKYCHYVAGLVGIGLSRLFSASEFEDPIVGEDTECANSMGLFLQKTNIIRDYLEDQQEGRQFWPQEVWGKYVKKLEDFVKPENVDVAVKCLNELITNALQHIPDVITYLSRLRNQSVFNFCAIPQVMAIATLAACYNNHQVFKGVVKIRKGQAVTLMMDATNMPAVKAIIYQYIEEIYHRVPNSDPSASKAKQLISNIRTQSLPNCQLISRSHYSPIYLSFIMLLAALSWQYLSTLSQVTEDYVQREH

Entrez Gene ID

29580

Gene Name

farnesyl diphosphate farnesyl transferase 1

Gene Symbol

Fdft1

Species

Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005783	IEA:UniProtKB-KW	C	endoplasmic reticulum
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0043231	IDA:RGD	C	intracellular membrane-bounded organelle
GO:0004310	IDA:RGD	F	farnesyl-diphosphate farnesyltransferase activity
GO:0016491	IEA:UniProtKB-KW	F	oxidoreductase activity
GO:0051996	IEA:UniProtKB-EC	F	squalene synthase activity
GO:0006695	IMP:RGD	P	cholesterol biosynthetic process
GO:0045338	IMP:RGD	P	farnesyl diphosphate metabolic process
GO:0008299	IEA:UniProtKB-KW	P	isoprenoid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
rno01100	Metabolic pathways
ko00100	Steroid biosynthesis
rno00100	Steroid biosynthesis

REACTOME Pathway Links

REACTOME Pathway ID	Description
5954496	Activation of gene expression by SREBF (SREBP)
5953924	Cholesterol biosynthesis
5953288	Fatty acid, triacylglycerol, and ketone body metabolism
5953250	Metabolism
5953289	Metabolism of lipids and lipoproteins
5954333	PPARA activates gene expression
5954497	Regulation of cholesterol biosynthesis by SREBP (SREBF)
5954222	Regulation of lipid metabolism by Peroxisome proliferator-activated receptor alpha (PPARalpha)

InterPro Annotations

Accession	Description
IPR006449	Farnesyl-diphosphate farnesyltransferase
IPR008949	Isoprenoid synthase domain
IPR002060	Squalene/phytoene synthase
IPR019845	Squalene/phytoene synthase, conserved site

Entry Information

Gene Name

farnesyl diphosphate farnesyl transferase 1

Protein Entry

FDFT_RAT

UniProt ID

Q02769

Species

Rat

Comments

Comment Type	Description
Catalytic Activity	2 farnesyl diphosphate + NAD(P)H = squalene + 2 diphosphate + NAD(P)(+).
Cofactor	Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Disruption Phenotype	Hypomorphic mutations in the Lss gene and in this gene were identified, as well as a null mutation in the Lss gene. Cataract onset is associated with the specific combination of Lss and Fdft1 mutant alleles that decrease cholesterol levels in cataractous lenses to about 57% of normal. Cholesterol insufficiency may cause the deficient proliferation of lens epithelial cells in Shumiya cataract rats, resulting in the loss of homeostatic epithelial cell control of the underlying fiber cells and ultimately cataractogenesis
Function	Critical branch point enzyme of isoprenoid biosynthesis that is thought to regulate the flux of isoprene intermediates through the sterol pathway.
Pathway	Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 1/3.
Similarity	Belongs to the phytoene/squalene synthase family
Subcellular Location	Endoplasmic reticulum membrane ; Multi-pass membrane protein .

Unique RefSeq proteins for LMP012613 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
9506591	RefSeq	NP_062111	416	squalene synthase

Identical Sequences to LMP012613 proteins

Reference	Database	Accession	Length	Protein Name
GI:9506591	GenBank	AAA42179.1	416	squalene synthetase [Rattus norvegicus]
GI:9506591	GenBank	AAH81810.1	416	Farnesyl diphosphate farnesyl transferase 1 [Rattus norvegicus]
GI:9506591	GenBank	EDL85317.1	416	farnesyl diphosphate farnesyl transferase 1 [Rattus norvegicus]
GI:9506591	SwissProt	Q02769.1	416	RecName: Full=Squalene synthase; Short=SQS; Short=SS; AltName: Full=FPP:FPP farnesyltransferase; AltName: Full=Farnesyl-diphosphate farnesyltransferase [Rattus norvegicus]

Related Sequences to LMP012613 proteins

Reference	Database	Accession	Length	Protein Name
GI:9506591	GenBank	AAH81810.1	416	Farnesyl diphosphate farnesyl transferase 1 [Rattus norvegicus]
GI:9506591	GenBank	EDL85317.1	416	farnesyl diphosphate farnesyl transferase 1 [Rattus norvegicus]
GI:9506591	SwissProt	Q02769.1	416	RecName: Full=Squalene synthase; Short=SQS; Short=SS; AltName: Full=FPP:FPP farnesyltransferase; AltName: Full=Farnesyl-diphosphate farnesyltransferase [Rattus norvegicus]