Gene/Proteome Database (LMPD)
LMPD ID
LMP011438
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
very-long-chain enoyl-CoA reductase
Gene Symbol
Synonyms
ATTSC13; CER10; ECERIFERUM 10; ECR; ENOYL-COA REDUCTASE; TSC13
Alternate Names
very-long-chain enoyl-CoA reductase
Chromosome
3
EC Number
1.3.1.93
Summary
Enoyl-CoA reductase is involved in all very long chain fatty acids (VLCFA) elongation reactions that are required for cuticular wax, storage lipid and sphingolipid metabolism. The protein is located in the ER, but in contrast to its yeast homolog TSC13 is not particularly enriched in the nuclear envelope-vacuole junction. Mutants in this gene show abnormal organ morphology and stem glossiness. Cells in all tissues are only about 1/3 of the size of wild type cells. The morphological changes are most likely to result from the reduction in the VLCFA content of sphingolipids. Mutants also show abnormalities in the endocytic membrane organization and transport.
Orthologs
Proteins
| very-long-chain enoyl-CoA reductase | |
|---|---|
| Refseq ID | NP_191096 |
| Protein GI | 15233250 |
| UniProt ID | Q9M2U2 |
| mRNA ID | NM_115394 |
| Length | 310 |
| RefSeq Status | REVIEWED |
| MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAKKFYPSRQRLTLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIYPVFYYFPVYKFLGYGEDCVIHPVQTYAMYYWCFHYFKRILETFFVHRFSHATSPIGNVFRNCAYYWSFGAYIAYYVNHPLYTPVSDLQMKIGFGFGLVCQVANFYCHILLKNLRDPSGAGGYQIPRGFLFNIVTCANYTTEIYQWLGFNIATQTIAGYVFLAVAALIMTNWALGKHSRLRKIFDGKDGKPKYPRRWVILPPFL | |
Gene Information
Entrez Gene ID
Gene Name
very-long-chain enoyl-CoA reductase
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005783 | IDA:TAIR | C | endoplasmic reticulum |
| GO:0005789 | IDA:TAIR | C | endoplasmic reticulum membrane |
| GO:0009923 | TAS:TAIR | C | fatty acid elongase complex |
| GO:0016021 | IEA:UniProtKB-KW | C | integral component of membrane |
| GO:0005886 | IDA:TAIR | C | plasma membrane |
| GO:0009922 | IMP:TAIR | F | fatty acid elongase activity |
| GO:0019166 | IGI:TAIR | F | trans-2-enoyl-CoA reductase (NADPH) activity |
| GO:0006633 | IEA:UniProtKB-UniPathway | P | fatty acid biosynthetic process |
| GO:0006665 | IMP:TAIR | P | sphingolipid metabolic process |
| GO:0010025 | IMP:TAIR | P | wax biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath01040 | Biosynthesis of unsaturated fatty acids |
| ath_M00415 | Fatty acid biosynthesis, elongation, endoplasmic reticulum |
| ath00062 | Fatty acid elongation |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 6253799 | Synthesis of very long-chain fatty acyl-CoAs |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR001104 | 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal |
UniProt Annotations
Entry Information
Gene Name
very-long-chain enoyl-CoA reductase
Protein Entry
ECR_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | A very-long-chain acyl-CoA + NADP(+) = a very- long-chain trans-2,3-dehydroacyl-CoA + NADPH. |
| Disruption Phenotype | Abnormal organ morphology and stem glossiness. {ECO:0000269|PubMed:15829606}. |
| Function | Enoyl-CoA reductase responsible for the second reduction step in very long-chain fatty acids (VLCFAs) synthesis. Required for the elongation of fatty acids precursors of sphingolipids, storage lipids and cuticular waxes. {ECO:0000269|PubMed:14673020, ECO:0000269|PubMed:15829606}. |
| Pathway | Lipid metabolism; fatty acid biosynthesis. |
| Similarity | Belongs to the steroid 5-alpha reductase family. {ECO:0000305}. |
| Subcellular Location | Endoplasmic reticulum membrane {ECO:0000269|PubMed:15829606}; Multi-pass membrane protein {ECO:0000269|PubMed:15829606}. |
| Subunit | Part of the fatty acid elongase complex which contains a beta-ketoacyl-CoA synthase (KCS), a beta-ketoacyl-CoA reductase (KCR), a beta-hydroxyacyl-CoA dehydratase (HCD) and an enoyl-CoA reductase (ECR). {ECO:0000305}. |
| Tissue Specificity | Expressed in roots, leaves, stems, flowers and siliques. Detected in mature seeds. {ECO:0000269|PubMed:14673020}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP011438 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15233250 | RefSeq | NP_191096 | 310 | very-long-chain enoyl-CoA reductase |
Identical Sequences to LMP011438 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15233250 | EMBL | CAB75894.1 | 310 | glycoprotein-like [Arabidopsis thaliana] |
| GI:15233250 | GenBank | AAL38312.1 | 310 | glycoprotein-like [Arabidopsis thaliana] |
| GI:15233250 | GenBank | AAM10145.1 | 310 | glycoprotein-like [Arabidopsis thaliana] |
| GI:15233250 | GenBank | AAM65988.1 | 310 | synaptic glycoprotein SC2-like protein [Arabidopsis thaliana] |
| GI:15233250 | GenBank | AEE79372.1 | 310 | very-long-chain enoyl-CoA reductase [Arabidopsis thaliana] |
| GI:15233250 | SwissProt | Q9M2U2.1 | 310 | RecName: Full=Very-long-chain enoyl-CoA reductase; AltName: Full=Enoyl-CoA reductase; Short=AtECR; AltName: Full=Protein ECERIFERUM 10; AltName: Full=Synaptic glycoprotein SC2-like protein [Arabidopsis thaliana] |
Related Sequences to LMP011438 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15233250 | EMBL | CDX73389.1 | 310 | BnaC08g26140D [Brassica napus] |
| GI:15233250 | GenBank | ACR56737.1 | 310 | trans-2,3-enoyl-CoA reductase [Brassica napus] |
| GI:15233250 | GenBank | ADL92437.1 | 309 | Sequence 3 from patent US 7709666 |
| GI:15233250 | GenBank | EOA23271.1 | 310 | hypothetical protein CARUB_v10017686mg [Capsella rubella] |
| GI:15233250 | RefSeq | XP_006290373.1 | 310 | hypothetical protein CARUB_v10017686mg [Capsella rubella] |
| GI:15233250 | RefSeq | XP_010504389.1 | 310 | PREDICTED: very-long-chain enoyl-CoA reductase [Camelina sativa] |