Gene/Proteome Database (LMPD)

LMPD ID
LMP011163
Gene ID
830731
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
S-isoprenylcysteine O-methyltransferase B
Gene Symbol
ATSTE14B
Synonyms
ARABIDOPSIS THALIANA ISOPRENYL CYSTEINE METHYLTRANSFERASE B; ATICMTB; ATSTE14B; ICMTB; ISOPRENYL CYSTEINE METHYLTRANSFERASE B
Alternate Names
S-isoprenylcysteine O-methyltransferase B
Chromosome
5
EC Number
2.1.1.100

Proteins

S-isoprenylcysteine O-methyltransferase B
Refseq ID NP_568191
Protein GI 18415785
UniProt ID Q93W54
mRNA ID NM_120917
Length 197
RefSeq Status REVIEWED
MTEIFSDTGFRQLTQMFLAIIFFHTSEYILAIAIHGASKVTLSSLLISKHYALAMLISVLEYIAEIVFFPGLKQHWWISNFGLTMIILGEILRKTAIITAGRSFTHLIKIRREEHHKLVTEGVYQIMRHPSYSGFLIWSVGTQVMLCNPISAIAFAVVVWRFFAERIPYEEHYLKQFFGRQYVEYAQRVPSGVPFVN

Gene Information

Entrez Gene ID
830731
Gene Name
S-isoprenylcysteine O-methyltransferase B
Gene Symbol
ATSTE14B
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0004671 IDA:TAIR F protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity
GO:0009908 IMP:TAIR P flower development
GO:0009788 NAS:TAIR P negative regulation of abscisic acid-activated signaling pathway
GO:0048367 IMP:TAIR P shoot system development

KEGG Pathway Links

KEGG Pathway ID Description
ath00900 Terpenoid backbone biosynthesis
ko00900 Terpenoid backbone biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR007269 Isoprenylcysteine carboxyl methyltransferase
IPR025770 Protein-S-isoprenylcysteine O-methyltransferase

UniProt Annotations

Entry Information

Gene Name
S-isoprenylcysteine O-methyltransferase B
Protein Entry
ICMTB_ARATH
UniProt ID
Q93W54
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=5.0 uM for AFC as methyl acceptor {ECO:0000269|PubMed:12472689}; KM=3.0 uM for AGGC as methyl acceptor {ECO:0000269|PubMed:12472689}; Vmax=220 pmol/min/mg enzyme toward AFC as methyl acceptor {ECO:0000269|PubMed:12472689}; Vmax=296 pmol/min/mg enzyme toward AGGC as methyl acceptor {ECO:0000269|PubMed:12472689};
Catalytic Activity S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C- terminal S-farnesyl-L-cysteine methyl ester.
Cofactor Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250}; Note=Divalent metal cations. Probably Zn(2+). {ECO:0000250};
Disruption Phenotype Plants lacking ICMTA and ICMTB have altered phyllotaxis, fasciated stems and development of axillary flowers. {ECO:0000269|PubMed:18641086}.
Enzyme Regulation Inhibited by farnesylthioacetic acid (FTAA) and N-acetyl-S-trans, trans-farnesyl-l-cysteine (AFC).
Function Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, resulting in the modulation of the function of prenylated proteins. Involved in negative regulation of abscisic acid signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins. {ECO:0000269|PubMed:12472689, ECO:0000269|PubMed:18957507}.
Induction Not induced by abscisic acid or auxin. {ECO:0000269|PubMed:18957507}.
Miscellaneous ICMTB is more widely expressed and has a higher catalytic activity than ICMTA.
Sequence Caution Sequence=CAC08334.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:18641086}; Multi-pass membrane protein {ECO:0000269|PubMed:18641086}.
Tissue Specificity Expressed in flowers, stems, leaves, roots and siliques. Detected in apices and vascular tissues of leaves and roots, in the stigma and in the filaments and anthers of stamen. Not found in petioles or hypocotyls. {ECO:0000269|PubMed:12472689}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011163 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18415785 RefSeq NP_568191 197 S-isoprenylcysteine O-methyltransferase B

Identical Sequences to LMP011163 proteins

Reference Database Accession Length Protein Name
GI:18415785 GenBank AAK59533.1 197 unknown protein [Arabidopsis thaliana]
GI:18415785 GenBank AAL15208.1 197 unknown protein [Arabidopsis thaliana]
GI:18415785 gnl TAIR 197 S-isoprenylcysteine O-methyltransferase B [Arabidopsis thaliana]
GI:18415785 SwissProt Q93W54.1 197 RecName: Full=Protein-S-isoprenylcysteine O-methyltransferase B; Short=AtICMTB; AltName: Full=Isoprenylcysteine carboxylmethyltransferase B; AltName: Full=Prenylated protein carboxyl methyltransferase B; AltName: Full=Prenylcysteine carboxyl methyltransferase B [Arabidopsis thaliana]

Related Sequences to LMP011163 proteins

Reference Database Accession Length Protein Name
GI:18415785 GenBank EFH47579.1 197 prenylcysteine alpha-carboxyl methyltransferase [Arabidopsis lyrata subsp. lyrata]
GI:18415785 GenBank EOA19466.1 197 hypothetical protein CARUB_v10002010mg [Capsella rubella]
GI:18415785 RefSeq XP_002871320.1 197 prenylcysteine alpha-carboxyl methyltransferase [Arabidopsis lyrata subsp. lyrata]
GI:18415785 RefSeq XP_006286568.1 197 hypothetical protein CARUB_v10002010mg [Capsella rubella]
GI:18415785 RefSeq XP_010423104.1 197 PREDICTED: protein-S-isoprenylcysteine O-methyltransferase B [Camelina sativa]
GI:18415785 RefSeq XP_010423106.1 197 PREDICTED: protein-S-isoprenylcysteine O-methyltransferase B [Camelina sativa]