Gene/Proteome Database (LMPD)

LMPD ID
LMP011162
Gene ID
832396
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
protein-S-isoprenylcysteine O-methyltransferase A
Gene Symbol
STE14A
Synonyms
ARABIDOPSIS THALIANA ISOPRENYL CYSTEINE METHYLTRANSFERASE A; ATICMTA; ATPCM; ATSTE14; ATSTE14A; homolog of yeast STE14 A; ICMTA; ISOPRENYL CYSTEINE METHYLTRANSFERASE A; LPHA-CARBOXYL METHYLTRANSFERASE; MKD15.18; MKD15_18; PRENYLCYSTEINE ALPHA-CARBOXYL METHYLTRANSFERASE; STE14; STE14A
Alternate Names
protein-S-isoprenylcysteine O-methyltransferase A
Chromosome
5
EC Number
2.1.1.100

Proteins

protein-S-isoprenylcysteine O-methyltransferase A
Refseq ID NP_197723
Protein GI 15237283
UniProt ID Q9FMW9
mRNA ID NM_122238
Length 197
RefSeq Status REVIEWED
MTEIFSDTSIRQLSQMLLSLIFFHISEYILAITIHGASNVTLSSLLITKHYALAMLLSLLEYLTEIILFPGLKQHWWVSNFGLIMIIVGEIIRKAAIITAGRSFTHLIKINYEEHHGLVTHGVYRLMRHPSYCGFLIWSVGTQVMLCNPVSAVAFAVVVWRFFAQRIPYEEYFLNQFFGVQYLEYAESVASGVPFVN

Gene Information

Entrez Gene ID
832396
Gene Name
protein-S-isoprenylcysteine O-methyltransferase A
Gene Symbol
STE14A
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0010340 IMP:TAIR F carboxyl-O-methyltransferase activity
GO:0004671 IDA:TAIR F protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity
GO:0006481 IDA:TAIR P C-terminal protein methylation
GO:0009908 IMP:TAIR P flower development
GO:0009788 NAS:TAIR P negative regulation of abscisic acid-activated signaling pathway
GO:0048367 IMP:TAIR P shoot system development

KEGG Pathway Links

KEGG Pathway ID Description
ath00900 Terpenoid backbone biosynthesis
ko00900 Terpenoid backbone biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR007269 Isoprenylcysteine carboxyl methyltransferase
IPR025770 Protein-S-isoprenylcysteine O-methyltransferase

UniProt Annotations

Entry Information

Gene Name
protein-S-isoprenylcysteine O-methyltransferase A
Protein Entry
ICMTA_ARATH
UniProt ID
Q9FMW9
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=22.7 uM for AFC as methyl acceptor {ECO:0000269|PubMed:12472689}; KM=13.7 uM for AGGC as methyl acceptor {ECO:0000269|PubMed:12472689}; Vmax=5.0 pmol/min/mg enzyme toward AFC as methyl acceptor {ECO:0000269|PubMed:12472689}; Vmax=3.7 pmol/min/mg enzyme toward AGGC as methyl acceptor {ECO:0000269|PubMed:12472689};
Catalytic Activity S-adenosyl-L-methionine + protein C-terminal S-farnesyl-L-cysteine = S-adenosyl-L-homocysteine + protein C- terminal S-farnesyl-L-cysteine methyl ester.
Cofactor Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250}; Note=Divalent metal cations. Probably Zn(2+). {ECO:0000250};
Disruption Phenotype No visible phenotype; due to redundancy with ICMTB. Icmta and icmtb double mutants have altered phyllotaxis, fasciated stems and development of axillary flowers. {ECO:0000269|PubMed:18641086}.
Enzyme Regulation Inhibited by farnesylthioacetic acid (FTAA) and N-acetyl-S-trans, trans-farnesyl-l-cysteine (AFC).
Function Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues, resulting in the modulation of the function of prenylated proteins. Involved in negative regulation of abscisic acid signaling. Carboxyl methylation is a reversible and potentially regulated step in the post-translational modification of prenylated proteins. {ECO:0000269|PubMed:11135111, ECO:0000269|PubMed:11352465, ECO:0000269|PubMed:12472689, ECO:0000269|PubMed:18957507}.
Induction Not induced by abscisic acid or auxin. {ECO:0000269|PubMed:18957507}.
Miscellaneous ICMTA is less widely expressed and has a lower catalytic activity than ICMTB.
Sequence Caution Sequence=ABK28709.1; Type=Erroneous termination; Positions=198; Note=Translated as stop.; Evidence={ECO:0000305};
Similarity Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:11135111, ECO:0000269|PubMed:18641086}; Multi- pass membrane protein {ECO:0000269|PubMed:11135111, ECO:0000269|PubMed:18641086}.
Tissue Specificity Expressed primarily in flowers, stems, leaves and roots. Almost not expressed in siliques. Detected in root tips and vascular tissues of roots, cotyledons, petiols, hypocotyls, filaments, pollen grains and the distal and proximal portions of the gynoecium. {ECO:0000269|PubMed:11352465, ECO:0000269|PubMed:12472689}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011162 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15237283 RefSeq NP_197723 197 protein-S-isoprenylcysteine O-methyltransferase A

Identical Sequences to LMP011162 proteins

Reference Database Accession Length Protein Name
GI:15237283 DBBJ BAB11187.1 197 farnesyl cysteine carboxyl methyltransferase-like protein [Arabidopsis thaliana]
GI:15237283 GenBank ABE66176.1 197 isoprenylcysteine carboxyl methyltransferase family protein/ICMT family protein [Arabidopsis thaliana]
GI:15237283 gnl TAIR 197 protein-S-isoprenylcysteine O-methyltransferase A [Arabidopsis thaliana]
GI:15237283 SwissProt Q9FMW9.1 197 RecName: Full=Protein-S-isoprenylcysteine O-methyltransferase A; Short=AtICMTA; AltName: Full=Isoprenylcysteine carboxylmethyltransferase A; AltName: Full=Prenylated protein carboxyl methyltransferase A; AltName: Full=Prenylcysteine carboxyl methyltransferase A; Short=AtPCM [Arabidopsis thaliana]

Related Sequences to LMP011162 proteins

Reference Database Accession Length Protein Name
GI:15237283 GenBank ABK28709.1 198 unknown, partial [Arabidopsis thaliana]
GI:15237283 GenBank EFH48310.1 197 prenylcysteine alpha-carboxyl methyltransferase 14A [Arabidopsis lyrata subsp. lyrata]
GI:15237283 RefSeq XP_002872051.1 197 prenylcysteine alpha-carboxyl methyltransferase 14A [Arabidopsis lyrata subsp. lyrata]
GI:15237283 RefSeq XP_010421132.1 222 PREDICTED: protein-S-isoprenylcysteine O-methyltransferase A [Camelina sativa]
GI:15237283 RefSeq XP_010421133.1 222 PREDICTED: protein-S-isoprenylcysteine O-methyltransferase A [Camelina sativa]
GI:15237283 RefSeq XP_010454613.1 261 PREDICTED: protein-S-isoprenylcysteine O-methyltransferase A-like [Camelina sativa]