LIPID MAPS

LMPD ID

LMP011156

Gene ID

838568

Species

Arabidopsis thaliana (Arabidopsis)

Gene Name

trigalactosyldiacylglycerol 1

Gene Symbol

TGD1

Synonyms

F14P1.27; F14P1_27; TGD1; trigalactosyldiacylglycerol 1

Alternate Names

trigalactosyldiacylglycerol 1

Chromosome

1

Summary

Encodes a permease-like protein involved in lipid transfer from the ER to the chloroplast, more specifically, transfer of phosphatidate across the chloroplast inner membrane. Mutant leaves accumulate trigalactosyldiacylglycerol, triacylglycerol and phosphatidate. Chloroplast lipids are altered in their fatty acid composition and as a consequence the development of chloroplasts in the mutants are impacted. The mutant seeds has a higher abortion rate. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.

Orthologs

View orthologs and multiple alignments for TGD1

trigalactosyldiacylglycerol 1
Refseq ID	NP_173410
Protein GI	15223672
UniProt ID	Q8L4R0
mRNA ID	NM_101836
Length	350
RefSeq Status	REVIEWED
MMQTCCIHQSFCFPHRVFPRFDASIGIKPPKLCQVGFIGKTQSYGISSPIRQRRLYVNLNANDGHPSMSMLEEETSTENNAPSQEAELPFSKWSPSKYIWRGLSVPIIAGQVVLRILKGKIHWRNTLQQLERTGPKSLGVCLLTSTFVGMAFTIQFVREFTRLGLNRSIGGVLALAFSRELSPVITSIVVAGRMGSAFAAELGTMQVSEQTDTLRVLGADPIDYLITPRVIASCLALPFLTLMCFTVGMASSALLSDAVYGISINIIMDSAHRALRPWDIVSAMIKSQVFGAIISVISCSWGVTTTGGAKGVGESTTSAVVMSLVGIFIADFVLSSFFFQGAGDSLKNCV

trigalactosyldiacylglycerol 1
Refseq ID	NP_973869
Protein GI	42571557
UniProt ID	Q8L4R0
mRNA ID	NM_202140
Length	350
RefSeq Status	REVIEWED
Protein sequence is identical to GI:15223672 (mRNA isoform)

trigalactosyldiacylglycerol 1
Refseq ID	NP_973868
Protein GI	42571555
UniProt ID	Q8L4R0
mRNA ID	NM_202139
Length	350
RefSeq Status	REVIEWED
Protein sequence is identical to GI:15223672 (mRNA isoform)

Entrez Gene ID

838568

Gene Name

trigalactosyldiacylglycerol 1

Gene Symbol

TGD1

Species

Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0009507	IDA:TAIR	C	chloroplast
GO:0009941	IDA:TAIR	C	chloroplast envelope
GO:0009706	TAS:TAIR	C	chloroplast inner membrane
GO:0009707	IDA:TAIR	C	chloroplast outer membrane
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0005319	IMP:TAIR	F	lipid transporter activity
GO:0006869	IMP:TAIR	P	lipid transport

InterPro Annotations

Accession	Description
IPR003453	Permease domain

Entry Information

Gene Name

trigalactosyldiacylglycerol 1

Protein Entry

TGD1_ARATH

UniProt ID

Q8L4R0

Species

Arabidopsis

Comments

Comment Type	Description
Caution	Was originally (PubMed:18299247) thought to belong to the ABC transporter family. Lacks the conserved ABC domain, which is one of the features of the ABC transporter family. {ECO:0000305\|PubMed:18299247}.
Disruption Phenotype	Disruption in the biosynthesis of ER-derived thylakoid lipids, and accumulation of oligogalactolipids, triacylglycerols (TAGs), and phosphatidates (PAs). Reduced growth with stout leaves and siliques. Impaired embryogenesis. {ECO:0000269\|PubMed:12743031, ECO:0000269\|PubMed:16199613}.
Function	Required during embryogenesis. Permease involved in lipid transfer from the endoplasmic reticulum (ER) to plastids, and necessary for thylakoids formation. {ECO:0000269\|PubMed:12743031, ECO:0000269\|PubMed:16199613, ECO:0000269\|PubMed:18689504}.
Sequence Caution	Sequence=AAG12551.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity	Belongs to the MlaE permease family. {ECO:0000305}.
Subcellular Location	Plastid, chloroplast inner membrane {ECO:0000269\|PubMed:12743031, ECO:0000269\|PubMed:16199613, ECO:0000269\|PubMed:18431481}; Multi-pass membrane protein {ECO:0000269\|PubMed:12743031, ECO:0000269\|PubMed:16199613, ECO:0000269\|PubMed:18431481}.
Subunit	Permease subunit of a probable lipid translocator at the inner chloroplast envelope membrane made of TGD1, TGD2 and TGD3. {ECO:0000305}.
Tissue Specificity	High levels in green tissues, but low levels in nongreen tissues such as roots. {ECO:0000269\|PubMed:12743031}.

Unique RefSeq proteins for LMP011156 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
15223672	RefSeq	NP_173410	350	trigalactosyldiacylglycerol 1

Identical Sequences to LMP011156 proteins

Reference	Database	Accession	Length	Protein Name
GI:15223672	DBBJ	BAH20061.1	350	AT1G19800 [Arabidopsis thaliana]
GI:15223672	GenBank	ACX19189.1	350	Sequence 47281 from patent US 7569389
GI:15223672	GenBank	AEE29899.1	350	trigalactosyldiacylglycerol 1 [Arabidopsis thaliana]
GI:15223672	GenBank	AEE29900.1	350	trigalactosyldiacylglycerol 1 [Arabidopsis thaliana]
GI:15223672	GenBank	AEE29901.1	350	trigalactosyldiacylglycerol 1 [Arabidopsis thaliana]
GI:15223672	SwissProt	Q8L4R0.1	350	RecName: Full=Protein TRIGALACTOSYLDIACYLGLYCEROL 1, chloroplastic; AltName: Full=ABC transporter I family member 14; Short=ABC transporter ABCI.14; Short=AtABCI14; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP011156 proteins

Reference	Database	Accession	Length	Protein Name
GI:15223672	GenBank	ACX19190.1	349	Sequence 47282 from patent US 7569389
GI:15223672	GenBank	EFH66613.1	350	hypothetical protein ARALYDRAFT_472206 [Arabidopsis lyrata subsp. lyrata]
GI:15223672	GenBank	EOA38117.1	352	hypothetical protein CARUB_v10009585mg [Capsella rubella]
GI:15223672	RefSeq	XP_002890354.1	350	hypothetical protein ARALYDRAFT_472206 [Arabidopsis lyrata subsp. lyrata]
GI:15223672	RefSeq	XP_006305219.1	352	hypothetical protein CARUB_v10009585mg [Capsella rubella]
GI:15223672	RefSeq	XP_010498403.1	359	PREDICTED: LOW QUALITY PROTEIN: protein TRIGALACTOSYLDIACYLGLYCEROL 1, chloroplastic-like [Camelina sativa]