Gene/Proteome Database (LMPD)

LMPD ID
LMP011091
Gene ID
837602
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
protein ECERIFERUM 1
Gene Symbol
CER1
Synonyms
AT1G02200; CER1; ECERIFERUM 1; POSSIBLE ALDEHYDE DECARBONYLASE; T6A9.16
Alternate Names
protein ECERIFERUM 1
Chromosome
1
Summary
Expression of the CER1 gene associated with production of stem epicuticular wax and pollen fertility. Biochemical studies showed that cer1 mutants are blocked in the conversion of stem wax C30 aldehydes to C29 alkanes, and they also lack the secondary alcohols and ketones. These suggested the CER1 protein is an aldehyde decarbonylase, but the exact molecular function of this protein remains to be determined.
Orthologs
View orthologs and multiple alignments for CER1

Proteins

protein ECERIFERUM 1
Refseq ID NP_001184890
Protein GI 334182234
UniProt ID F4HVY0
mRNA ID NM_001197961
Length 630
RefSeq Status REVIEWED
MATKPGVLTDWPWTPLGSFKYIVIAPWAVHSTYRFVTDDPEKRDLGYFLVFPFLLFRILHNQVWISLSRYYTSSGKRRIVDKGIDFNQVDRETNWDDQILFNGVLFYIGINLLPEAKQLPWWRTDGVLMAALIHTGPVEFLYYWLHKALHHHFLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFILFAIPLLTTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFHLFPPLKFLCYTPSYHSLHHTQFRTNYSLFMPLYDYIYGTMDESTDTLYEKTLERGDDIVDVVHLTHLTTPESIYHLRIGLASFASYPFAYRWFMRLLWPFTSLSMIFTLFYARLFVAERNSFNKLNLQSWVIPRYNLQYLLKWRKEAINNMIEKAILEADKKGVKVLSLGLMNQGEELNRNGEVYIHNHPDMKVRLVDGSRLAAAVVINSVPKATTSVVMTGNLTKVAYTIASALCQRGVQVSTLRLDEYEKIRSCVPQECRDHLVYLTSEALSSNKGFWVKVWLVGEGTTREEQEKATKGTLFIPFSQFPLKQLRRDCIYHTTPALIVPKSLVNVHSCENWLPRKAMSATRVAGILHALEGWEMHECGTSLLLSDLDQVWEACLSHGFQPLLLPHH
protein ECERIFERUM 1
Refseq ID NP_171723
Protein GI 30678265
UniProt ID F4HVY0
mRNA ID NM_100101
Length 625
RefSeq Status REVIEWED
MATKPGVLTDWPWTPLGSFKYIVIAPWAVHSTYRFVTDDPEKRDLGYFLVFPFLLFRILHNQVWISLSRYYTSSGKRRIVDKGIDFNQVDRETNWDDQILFNGVLFYIGINLLPEAKQLPWWRTDGVLMAALIHTGPVEFLYYWLHKALHHHFLYSRYHSHHHSSIVTEPITSVIHPFAEHIAYFILFAIPLLTTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFHLFPPLKFLCYTPSYHSLHHTQFRTNYSLFMPLYDYIYGTMDESTDTLYEKTLERGDDIVDVVHLTHLTTPESIYHLRIGLASFASYPFAYRWFMRLLWPFTSLSMIFTLFYARLFVAERNSFNKLNLQSWVIPRYNLQYLLKWRKEAINNMIEKAILEADKKGVKVLSLGLMNQGEELNRNGEVYIHNHPDMKVRLVDGSRLAAAVVINSVPKATTSVVMTGNLTKVAYTIASALCQRGVQVSTLRLDEYEKIRSCVPQECRDHLVYLTSEALSSNKVWLVGEGTTREEQEKATKGTLFIPFSQFPLKQLRRDCIYHTTPALIVPKSLVNVHSCENWLPRKAMSATRVAGILHALEGWEMHECGTSLLLSDLDQVWEACLSHGFQPLLLPHH
protein ECERIFERUM 1
Refseq ID NP_850932
Protein GI 145361948
UniProt ID F4HVY0
mRNA ID NM_180601
Length 461
RefSeq Status REVIEWED
MGFDQMYAAVIHPFAEHIAYFILFAIPLLTTLLTKTASIISFAGYIIYIDFMNNMGHCNFELIPKRLFHLFPPLKFLCYTPSYHSLHHTQFRTNYSLFMPLYDYIYGTMDESTDTLYEKTLERGDDIVDVVHLTHLTTPESIYHLRIGLASFASYPFAYRWFMRLLWPFTSLSMIFTLFYARLFVAERNSFNKLNLQSWVIPRYNLQYLLKWRKEAINNMIEKAILEADKKGVKVLSLGLMNQGEELNRNGEVYIHNHPDMKVRLVDGSRLAAAVVINSVPKATTSVVMTGNLTKVAYTIASALCQRGVQVSTLRLDEYEKIRSCVPQECRDHLVYLTSEALSSNKVWLVGEGTTREEQEKATKGTLFIPFSQFPLKQLRRDCIYHTTPALIVPKSLVNVHSCENWLPRKAMSATRVAGILHALEGWEMHECGTSLLLSDLDQVWEACLSHGFQPLLLPHH

Gene Information

Entrez Gene ID
837602
Gene Name
protein ECERIFERUM 1
Gene Symbol
CER1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005506 IEA:InterPro F iron ion binding
GO:0016491 IEA:InterPro F oxidoreductase activity
GO:0006633 IEA:InterPro P fatty acid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01110 Biosynthesis of secondary metabolites
ath00073 Cutin, suberine and wax biosynthesis
ko00073 Cutin, suberine and wax biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR006694 Fatty_acid_hydroxylase
IPR021940 Uncharacterised domain Wax2, C-terminal

UniProt Annotations

Entry Information

Gene Name
protein ECERIFERUM 1
Protein Entry
CER1_ARATH
UniProt ID
F4HVY0
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=1; IsoId=F4HVY0-1; Sequence=Displayed; Name=2; IsoId=F4HVY0-2; Sequence=VSP_044264; Note=Derived from EST data. No experimental confirmation available.; Name=3; IsoId=F4HVY0-3; Sequence=VSP_044263; Note=No experimental confirmation available.;
Disruption Phenotype Glossy stem and fruit and reduced fertility due to the inability of the pollen grains to rehydrate on the stigma surface. Disappearance of the wax crystals. Decreased C29, C31 and C33 alkane contents. Increased susceptibility to soil water deficit. {ECO:0000269|PubMed:21386033, ECO:0000269|PubMed:8718622}.
Function Aldehyde decarbonylase involved in the conversion of aldehydes to alkanes. Core component of a very-long-chain alkane synthesis complex. Involved in epicuticular wax biosynthesis and pollen fertility. {ECO:0000269|PubMed:21386033, ECO:0000269|PubMed:22773744, ECO:0000269|PubMed:8718622}.
Induction Down regulated by cold and dark stresses. Up-regulated by low humidity, osmotic stress and abscisic acid treatment. No effet of methyl jasmonate, GA3, salicylic acid, cytokinin or auxin treatments. {ECO:0000269|PubMed:19819982, ECO:0000269|PubMed:21386033}.
Sequence Caution Sequence=AAC24374.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=BAA11024.1; Type=Erroneous termination; Positions=588; Note=Translated as Leu.; Evidence={ECO:0000305}; Sequence=BAA11024.1; Type=Frameshift; Positions=560; Evidence={ECO:0000305};
Similarity Belongs to the sterol desaturase family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000269|PubMed:19892830, ECO:0000269|PubMed:22773744}; Multi- pass membrane protein {ECO:0000269|PubMed:19892830, ECO:0000269|PubMed:22773744}.
Subunit Homodimer. Interacts with CER3, CYTB5-B, CYTB5-C, CYTB5-D and CYTB5-E. {ECO:0000269|PubMed:22773744}.
Tissue Specificity Expressed in seedlings, stems, leaves, flowers, fruits and siliques. Not detected in roots, pollen and seeds. Expressed in trichomes, cotyledons, shoot apical meristem and leaf primordia. Preferentially associated with young leaves rather than mature leaves. Expressed in the epidermis of the stem and caulines leaves, in the carpels and the sepals. {ECO:0000269|PubMed:21386033, ECO:0000269|PubMed:8718622}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011091 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
334182234 RefSeq NP_001184890 630 protein ECERIFERUM 1
30678265 RefSeq NP_171723 625 protein ECERIFERUM 1
145361948 RefSeq NP_850932 461 protein ECERIFERUM 1

Identical Sequences to LMP011091 proteins

Reference Database Accession Length Protein Name
GI:145361948 DBBJ BAE98972.1 461 CER1 protein [Arabidopsis thaliana]
GI:30678265 GenBank ACX17497.1 625 Sequence 44996 from patent US 7569389
GI:145361948 GenBank AEE27400.1 461 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:30678265 GenBank AEE27401.1 625 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:334182234 GenBank AEE27402.1 630 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:30678265 SwissProt F4HVY0.1 625 RecName: Full=Protein ECERIFERUM 1 [Arabidopsis thaliana]

Related Sequences to LMP011091 proteins

Reference Database Accession Length Protein Name
GI:30678265 GenBank AAB87721.1 625 maize gl1 homolog [Arabidopsis thaliana]
GI:334182234 GenBank AAB87721.1 625 maize gl1 homolog [Arabidopsis thaliana]
GI:145361948 GenBank ACX17496.1 628 Sequence 44995 from patent US 7569389
GI:334182234 GenBank ACX17496.1 628 Sequence 44995 from patent US 7569389
GI:334182234 GenBank ACX17497.1 625 Sequence 44996 from patent US 7569389
GI:145361948 GenBank ACX17497.1 625 Sequence 44996 from patent US 7569389
GI:145361948 GenBank ACX17498.1 497 Sequence 44997 from patent US 7569389
GI:145361948 GenBank AEE27401.1 625 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:334182234 GenBank AEE27401.1 625 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:30678265 GenBank AEE27402.1 630 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:30678265 GenBank EOA39869.1 647 hypothetical protein CARUB_v10008547mg [Capsella rubella]
GI:30678265 GenBank AIE57503.1 625 CER1 [Camelina sativa]
GI:145361948 RefSeq NP_171723.2 625 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:334182234 RefSeq NP_171723.2 625 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:30678265 RefSeq NP_001184890.1 630 protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:30678265 RefSeq XP_006306971.1 647 hypothetical protein CARUB_v10008547mg [Capsella rubella]
GI:334182234 SwissProt F4HVY0.1 625 RecName: Full=Protein ECERIFERUM 1 [Arabidopsis thaliana]
GI:145361948 SwissProt F4HVY0.1 625 RecName: Full=Protein ECERIFERUM 1 [Arabidopsis thaliana]