Gene/Proteome Database (LMPD)

LMPD ID
LMP011069
Gene ID
837193
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
carotenoid isomerase
Gene Symbol
CRTISO
Synonyms
CAROTENOID AND CHLOROPLAST REGULATION 2; carotenoid isomerase; CAROTENOID ISOMERASE; CCR2; CRTISO; F4H5.10; F4H5_10
Alternate Names
carotenoid isomerase
Chromosome
1
EC Number
5.2.1.13
Summary
Encodes carotenoid isomerase. Catalyzes the isomerization of poly-cis-carotenoids to all-trans-carotenoids. Together with PDS and ZDS, CRTiso is required to complete the synthesis of lycopene from phytoene.
Orthologs
View orthologs and multiple alignments for CRTISO

Proteins

carotenoid isomerase
Refseq ID NP_172167
Protein GI 42561764
UniProt ID Q9M9Y8
mRNA ID NM_100559
Length 595
RefSeq Status REVIEWED
MDLCFQNPVKCGDRLFSALNTSTYYKLGTSNLGFNGPVLENRKKKKKLPRMVTVKSVSSSVVASTVQGTKRDGGESLYDAIVIGSGIGGLVAATQLAVKEARVLVLEKYLIPGGSSGFYERDGYTFDVGSSVMFGFSDKGNLNLITQALKAVGRKMEVIPDPTTVHFHLPNNLSVRIHREYDDFIAELTSKFPHEKEGILGFYGDCWKIFNSLNSLELKSLEEPIYLFGQFFQKPLECLTLAYYLPQNAGAIARKYIKDPQLLSFIDAECFIVSTVNALQTPMINASMVLCDRHYGGINYPVGGVGGIAKSLAEGLVDQGSEIQYKANVKSIILDHGKAVGVRLADGREFFAKTIISNATRWDTFGKLLKGEKLPKEEENFQKVYVKAPSFLSIHMGVKAEVLPPDTDCHHFVLEDDWKNLEEPYGSIFLSIPTILDSSLAPDGRHILHIFTTSSIEDWEGLPPKEYEAKKEDVAARIIQRLEKKLFPGLSSSITFKEVGTPRTHRRFLARDKGTYGPMPRGTPKGLLGMPFNTTAIDGLYCVGDSCFPGQGVIAVAFSGVMCAHRVAADIGLEKKSRVLDVGLLGLLGWLRTLA

Gene Information

Entrez Gene ID
837193
Gene Name
carotenoid isomerase
Gene Symbol
CRTISO
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IBA:RefGenome C chloroplast
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0046608 IMP:TAIR F carotenoid isomerase activity
GO:0016117 IGI:TAIR P carotenoid biosynthetic process
GO:0009662 IMP:TAIR P etioplast organization

KEGG Pathway Links

KEGG Pathway ID Description
ath01110 Biosynthesis of secondary metabolites
ath00906 Carotenoid biosynthesis
ath01100 Metabolic pathways
ath_M00097 beta-Carotene biosynthesis, GGAP => beta-carotene

Domain Information

InterPro Annotations

Accession Description
IPR014101 Carotene isomerase

UniProt Annotations

Entry Information

Gene Name
carotenoid isomerase
Protein Entry
CRTSO_ARATH
UniProt ID
Q9M9Y8
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity 7,9,7',9'-tetra-cis-lycopene = all-trans- lycopene. {ECO:0000269|PubMed:11884677}.
Cofactor Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000250}; Name=NADP(+); Xref=ChEBI:CHEBI:58349; Evidence={ECO:0000250}; Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250};
Function Carotene cis-trans-isomerase that converts 7,9,9'-tri- cis-neurosporene to 9'-cis-neurosporene and 7,9,9',7'-tetra-cis- lycopene (also known as prolycopene) into all-trans-lycopene. Isomerization requires redox-active components, suggesting that isomerization is achieved by a reversible redox reaction acting at specific double bonds. Isomerizes adjacent cis-double bonds at C7 and C9 pairwise into the trans-configuration, but is incapable of isomerizing single cis-double bonds at C9 and C9'. Carotenoid biosynthesis is partly required to form the prolamellar bodies of etioplasts. {ECO:0000269|PubMed:11884677}.
Pathway Carotenoid biosynthesis; lycopene biosynthesis. {ECO:0000269|PubMed:11884677}.
Sequence Caution Sequence=AAF63149.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the carotenoid/retinoid oxidoreductase family. CrtISO subfamily. {ECO:0000305}.
Subcellular Location Plastid, chloroplast membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP011069 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
42561764 RefSeq NP_172167 595 carotenoid isomerase

Identical Sequences to LMP011069 proteins

Reference Database Accession Length Protein Name
GI:42561764 EMBL CAW65957.1 595 unnamed protein product [Arabidopsis thaliana]
GI:42561764 EMBL CBC31594.1 595 unnamed protein product [Arabidopsis thaliana]
GI:42561764 EMBL CBD24329.1 595 unnamed protein product [Arabidopsis thaliana]
GI:42561764 EMBL CBD31487.1 595 unnamed protein product [Arabidopsis thaliana]
GI:42561764 EMBL CBC50416.1 595 unnamed protein product [Arabidopsis thaliana]
GI:42561764 GenBank AEE28042.1 595 carotenoid isomerase [Arabidopsis thaliana]

Related Sequences to LMP011069 proteins

Reference Database Accession Length Protein Name
GI:42561764 GenBank AAF63149.1 587 Similar to phytoene dehydrogenase [Arabidopsis thaliana]
GI:42561764 GenBank ACX04766.1 595 Sequence 27708 from patent US 7569389
GI:42561764 GenBank ACX21113.1 595 Sequence 49880 from patent US 7569389
GI:42561764 GenBank EFH68619.1 590 predicted protein [Arabidopsis lyrata subsp. lyrata]
GI:42561764 GenBank EOA39949.1 598 hypothetical protein CARUB_v10008639mg [Capsella rubella]
GI:42561764 RefSeq XP_002892360.1 590 predicted protein [Arabidopsis lyrata subsp. lyrata]