Gene/Proteome Database (LMPD)
LMPD ID
LMP010871
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
phospholipase D alpha 2
Gene Symbol
Synonyms
F6D8.21; F6D8_21; PHOSPHOLIPASE D; phospholipase D alpha 2; PLDALPHA2
Alternate Names
phospholipase D alpha 2
Chromosome
1
EC Number
3.1.4.4
Summary
member of C2-PLD subfamily
Orthologs
Proteins
| phospholipase D alpha 2 | |
|---|---|
| Refseq ID | NP_175666 |
| Protein GI | 15219031 |
| UniProt ID | Q9SSQ9 |
| mRNA ID | NM_104135 |
| Length | 810 |
| RefSeq Status | REVIEWED |
| MEECLLHGRLHATIYEVDHLHAEGGRSGFLGSILANVEETIGVGKGETQLYATIDLEKARVGRTRKITKEPKNPKWFESFHIYCGHMAKHVIFTVKDANPIGATLIGRGYIPVEDILHGEEVDRWVDILDNEKNPIAGGSKIHVKLQYFGVEKDKNWNRGIKSAKFPGVPYTFFSQRRGCKVSLYQDAHIPGNFVPKIPLAGGKNYEPHRCWEDIFDAITNAKHLIYITGWSVYTEISLVRDSRRPKQGGDVTVGELLKKKASEGVKVILLVWDDRTSVDLLKKDGLMATHDEETENFFRGTDVNCILCPRNPDDGGSIVQNLQISTMFTHHQKIVVVDSEMPSGGSRSRRIVSFVGGLDLCDGRYDTPFHSLFRTLDTAHHDDFHQPNFTGAAITKGGPREPWHDIHCRLEGPIAWDVLYNFEQRWSRQGGKDILVKMRELGDIIIPPSPVLFSEDHDVWNVQLFRSIDGGAAAGFPDSPEAAAEAGLVSGKDNIIDRSIQDAYIHAIRRAKDFIYIENQYFLGSSFAWSADGIKPEEINALHLIPKELSLKIVSKIKAGEKFKVYVVVPMWPEGIPESGSVQAILDWQKRTMEMMYKDVIKALRENGLEGEDPRDYLTFFCLGNREVKKDGEYEPSEKPEPDTDYIRAQEARRFMIYVHTKMMIVDDEYIIIGSANINQRSMDGARDSEIAMGGYQPYHLSTRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECIQKVNRVADKYWDLYSSESLEHDLPGHLLRYPIGIASEGNITELPGCEFFPDTKARILGVKSDYMPPILTT | |
Gene Information
Entrez Gene ID
Gene Name
phospholipase D alpha 2
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0009941 | IDA:TAIR | C | chloroplast envelope |
| GO:0031410 | IEA:UniProtKB-KW | C | cytoplasmic vesicle |
| GO:0016020 | IEA:UniProtKB-KW | C | membrane |
| GO:0005773 | IEA:UniProtKB-KW | C | vacuole |
| GO:0070290 | IEA:UniProtKB-EC | F | N-acylphosphatidylethanolamine-specific phospholipase D activity |
| GO:0005509 | IEA:InterPro | F | calcium ion binding |
| GO:0004630 | IEA:UniProtKB-EC | F | phospholipase D activity |
| GO:0009738 | IEA:UniProtKB-KW | P | abscisic acid-activated signaling pathway |
| GO:0009873 | IEA:UniProtKB-KW | P | ethylene-activated signaling pathway |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0046470 | IEA:InterPro | P | phosphatidylcholine metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath04144 | Endocytosis |
| ath00565 | Ether lipid metabolism |
| ath00564 | Glycerophospholipid metabolism |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| 6254320 | Synthesis of PG |
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | A phosphatidylcholine + H(2)O = choline + a phosphatidate. |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Ca(2+) requirement for activity depends on pH. Active either under acidic conditions with micromolar levels of calcium (PIP2- dependent) or at neutral pH with millimolar levels of calcium (PIP2-independent).; |
| Domain | C2 domain is a calcium-binding fold, and the binding promotes the protein association with membranes. A lower affinity toward calcium can be anticipated for PLD alpha due to the absence of two potential calcium ligands. |
| Function | Hydrolyzes glycerol-phospholipids at the terminal phosphodiesteric bond. Plays an important role in various cellular processes, including phytohormone action and response to stress, characterized by acidification of the cell. |
| Induction | Activated by abscisic acid (ABA), ethylene, heavy metal, cold, salt and osmotic stresses. |
| Similarity | Belongs to the phospholipase D family. C2-PLD subfamily. {ECO:0000305}. |
| Similarity | Contains 1 C2 domain. {ECO:0000305}. |
| Similarity | Contains 2 PLD phosphodiesterase domains. {ECO:0000255|PROSITE-ProRule:PRU00153}. |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:10198096}. Membrane {ECO:0000269|PubMed:10198096}; Peripheral membrane protein {ECO:0000269|PubMed:10198096}. Vacuole {ECO:0000269|PubMed:10198096}. Cytoplasmic vesicle, clathrin- coated vesicle {ECO:0000269|PubMed:10198096}. Note=Found in vacuoles and also associated with plasma, microsomal and mitochondrial membranes and in clathrin-coated vesicles. Not found in chloroplast or nuclei. Activation increases association of preexisting enzyme with membranes. The distribution of this conventional PLD between membrane-associated and soluble fractions varied from organ to organ and is calcium-regulated. |
| Tissue Specificity | Highly expressed in roots, stems and flowers, moderately in leaves, seedlings and siliques. Not detected in dry seeds. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010871 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 15219031 | RefSeq | NP_175666 | 810 | phospholipase D alpha 2 |
Identical Sequences to LMP010871 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15219031 | GenBank | AAD55607.1 | 810 | Similar to gb|AF090445 phospholipase D1 from Brassica oleacea [Arabidopsis thaliana] |
| GI:15219031 | GenBank | AEE32825.1 | 810 | phospholipase D alpha 2 [Arabidopsis thaliana] |
| GI:15219031 | SwissProt | Q9SSQ9.1 | 810 | RecName: Full=Phospholipase D alpha 2; Short=AtPLDalpha2; Short=PLD alpha 2; AltName: Full=Choline phosphatase 2; AltName: Full=Phosphatidylcholine-hydrolyzing phospholipase D 2 [Arabidopsis thaliana] |
Related Sequences to LMP010871 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15219031 | GenBank | EFH70647.1 | 810 | predicted protein [Arabidopsis lyrata subsp. lyrata] |
| GI:15219031 | GenBank | EOA39681.1 | 813 | hypothetical protein CARUB_v10008323mg, partial [Capsella rubella] |
| GI:15219031 | RefSeq | XP_002894388.1 | 810 | predicted protein [Arabidopsis lyrata subsp. lyrata] |
| GI:15219031 | RefSeq | XP_006306783.1 | 813 | hypothetical protein CARUB_v10008323mg, partial [Capsella rubella] |
| GI:15219031 | RefSeq | XP_010462103.1 | 810 | PREDICTED: phospholipase D alpha 2 [Camelina sativa] |
| GI:15219031 | RefSeq | XP_010462104.1 | 810 | PREDICTED: phospholipase D alpha 2 [Camelina sativa] |