Gene/Proteome Database (LMPD)

LMPD ID
LMP010825
Gene ID
830333
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
Myotubularin 2
Gene Symbol
AT5G04540
Synonyms
T32M21.150; T32M21_150
Alternate Names
Myotubularin 2
Chromosome
5
EC Number
3.1.3.95

Proteins

Myotubularin 2
Refseq ID NP_196074
Protein GI 42567644
UniProt ID F4JWB3
mRNA ID NM_120536
Length 833
RefSeq Status REVIEWED
MTALRPLSGRSRSLRCSSEKMEGTGSWDVLEWTKLDSASWSGSYSNLDCLLESERIIFEACGVILINTDEAGTLLLSNFRILFLSEGTRKLVPLGTIPFVAIEKFNKLAPKVQSNKYHNNENAPTRLLQVTGKDMRIVVYGFRPGTKQRHTVVDTLLRCNKPERVWDLYAFTCGPSQFGNTNPKERLLNEYFRLLGKSSQRASMNMIEDGSFTLSNDLWRITNLNSNYDLCQSYPFALMVPKSISDEELLQTSTFRARCRLPVISWCHPGSGAVIARSSQPLVGLMMNMRSNSDEKLVASFCTQLAGHKGARRKLYIVDARPRKNALANGAKGGGSESSSNYLQSEIVFLGIDNIHAMRESFSRLRDYLDMHGTTSSDGTSSFLRHGGWTWGGGNLSSMSASVSVLGDSGWLSHIQSILAGVAWIAARVAMESASVLVHCSDGWDRTTQLVSLACLLLDPYYRTFSGFQALVEKDWLSFGHPFSDRVGMPNVSESGNFELPIQSSSARSFPSSPVRQSPGSAAAQSSSSSYGLNNYSPIFLQWLDCISQLMRMYPSAFEFSPTFLVDFIDCLLSCRFGNFLCNSEKERQQCGISETCGCIWAYLADLRSSSGTSHVHCNPFYDPSRYDGPLLPPAAALAPTLWPQFHLRWACPVEPNVTETEDQCRAMTVKYSEMKKEKEEAERKVDELSSAMESLNEELLNERDISRAARESAKRATKERAVISRAVQSLGCKVKFTRNGDCTVEVEDGPQKCSHSIPQKQSEDNTTDVSESISSVTEQNVCEAVCPLRTREGTCRWPDAGCARIGNQFLGLKTNFEAFDNLCVYDSYFTAE

Gene Information

Entrez Gene ID
830333
Gene Name
Myotubularin 2
Gene Symbol
AT5G04540
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IDA:UniProtKB C cytoplasm
GO:0052629 IDA:UniProtKB F phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity
GO:0004438 IDA:UniProtKB F phosphatidylinositol-3-phosphatase activity
GO:0004725 IEA:InterPro F protein tyrosine phosphatase activity
GO:0035556 IMP:UniProtKB P intracellular signal transduction

REACTOME Pathway Links

REACTOME Pathway ID Description
6254334 PI Metabolism
6254338 Synthesis of PIPs at the early endosome membrane
6254335 Synthesis of PIPs at the late endosome membrane
6254333 Synthesis of PIPs at the plasma membrane

Domain Information

InterPro Annotations

Accession Description
IPR004182 GRAM domain
IPR010569 Myotubularin-like phosphatase domain
IPR011993 Pleckstrin homology-like domain
IPR016130 Protein-tyrosine phosphatase, active site
IPR029021 Protein-tyrosine phosphatase-like

UniProt Annotations

Entry Information

Gene Name
Myotubularin 2
Protein Entry
MYTM2_ARATH
UniProt ID
F4JWB3
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=216.5 uM for PtdIns3P {ECO:0000269|PubMed:22324391}; KM=158.2 uM for PtdIns3,5P(2) {ECO:0000269|PubMed:22324391}; Vmax=15.4 pmol/min/mg enzyme with PtdIns3P as substrate {ECO:0000269|PubMed:22324391}; Vmax=28.4 pmol/min/mg enzyme with PtdIns3,5P(2) as substrate {ECO:0000269|PubMed:22324391};
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 3,5- bisphosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol 5-phosphate + phosphate. {ECO:0000269|PubMed:22324391}.
Catalytic Activity 1-phosphatidyl-1D-myo-inositol 3-phosphate + H(2)O = 1-phosphatidyl-1D-myo-inositol + phosphate. {ECO:0000269|PubMed:22324391}.
Developmental Stage Expressed in young seedlings, especially at the tip of the growing shoot meristems. Later observed in roots and in aerial parts. Weakly expressed in leaves with local higher levels in the trichomes and in cotyledon veins. Present at low levels in flowers with higher accumulation in cells at organ-stem junctions. Restricted to the developing peduncle. {ECO:0000269|PubMed:22324391}.
Disruption Phenotype No visible phenotype in both normal and dehydration conditions. {ECO:0000269|PubMed:22324391}.
Function Phosphatase with phosphoinositide 3'-phosphatase activity that can use phosphatidylinositol-3-phosphate (PtdIns3P) and phosphatidylinositol-3,5-diphosphate (PtdIns3,5P(2)) as substrates and produces phosphatidylinositol-5-phosphate (PtdIns5P); participates in pathway(s) that transfer gene regulatory signals to the nucleus. {ECO:0000269|PubMed:22324391}.
Sequence Caution Sequence=CAB85561.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the protein-tyrosine phosphatase family. Non-receptor class myotubularin subfamily. {ECO:0000305}.
Similarity Contains 1 GRAM domain. {ECO:0000305}.
Similarity Contains 1 myotubularin phosphatase domain. {ECO:0000255|PROSITE-ProRule:PRU00669}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:22324391}. Note=Highly concentrated at the peripheral lobes of the epidermal cells.
Tissue Specificity Mostly expressed in flowers and roots, and, to a lower extent, in siliques and leaves. {ECO:0000269|PubMed:22324391}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010825 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
42567644 RefSeq NP_196074 833 Myotubularin 2

Identical Sequences to LMP010825 proteins

Reference Database Accession Length Protein Name
GI:42567644 gnl TAIR 833 Myotubularin 2 [Arabidopsis thaliana]
GI:42567644 SwissProt F4JWB3.1 833 RecName: Full=Phosphatidylinositol-3-phosphatase myotubularin-2; Short=AtMTM2; AltName: Full=Phosphatidylinositol-3,5-bisphosphate 3-phosphatase; AltName: Full=Phosphatidylinositol-3-phosphate phosphatase [Arabidopsis thaliana]

Related Sequences to LMP010825 proteins

Reference Database Accession Length Protein Name
GI:42567644 DBBJ BAF01096.1 833 myotubularin - like protein [Arabidopsis thaliana]
GI:42567644 EMBL CAB85561.1 799 myotubularin-like protein [Arabidopsis thaliana]
GI:42567644 GenBank EFH49414.1 833 inositol or phosphatidylinositol phosphatase/ phosphoric monoester hydrolase [Arabidopsis lyrata subsp. lyrata]
GI:42567644 RefSeq XP_002873155.1 833 inositol or phosphatidylinositol phosphatase/ phosphoric monoester hydrolase [Arabidopsis lyrata subsp. lyrata]
GI:42567644 RefSeq XP_010452352.1 833 PREDICTED: phosphatidylinositol-3-phosphatase myotubularin-2 [Camelina sativa]
GI:42567644 RefSeq XP_010490968.1 833 PREDICTED: phosphatidylinositol-3-phosphatase myotubularin-2-like [Camelina sativa]