Gene/Proteome Database (LMPD)

LMPD ID
LMP010813
Gene ID
829610
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
phosphatidylinositol transfer protein COW1
Gene Symbol
COW1
Synonyms
CAN OF WORMS1; COW1; SHORT ROOT HAIR 1; SRH1; T4L20.160; T4L20_160
Alternate Names
phosphatidylinositol transfer protein COW1
Chromosome
4
Summary
Encodes COW1 (can of worms1), a phosphatidylinositol transfer protein essential for root hair tip growth. The N-terminus of the COW1 protein is 32% identical to an essential phosphatidylinositol transfer protein (PITP), the yeast Sec14 protein (sec14p) while the C-terminus is 34.5% identical to a late nodulin of Lotus japonicus, Nlj16. Expression of COW1 complements the growth defect associated with Sec14p dysfunction in yeast. GFP fused to the COW1 protein specifically accumulates at the site of root hair outgrowth.
Orthologs
View orthologs and multiple alignments for COW1

Proteins

phosphatidylinositol transfer protein COW1
Refseq ID NP_195184
Protein GI 79497100
UniProt ID F4JLE5
mRNA ID NM_119624
Length 554
RefSeq Status REVIEWED
MAETKPEIEMSEEERKIVKISSLKKKAINASNRFKNSFKKKGRRSSSRVMSVPIEDDIDAEDLQALDAFRQALILDELLPSKLDDLHMMLRFLRARKFDIEKAKQMWSDMIQWRKDFGADTIIEDFDFEEIDEVMKHYPQGYHGVDKEGRPVYIERLGQIDANKLLQVTTMDRYVKYHVKEFEKTFKVKFPSCSVAANKHIDQSTTILDVQGVGLKNFSKSARELLQRLCKIDNENYPETLNRMFIINAGSGFRLLWSTVKSFLDPKTTAKIHVLGNKYHSKLLEVIDASELPEFFGGACTCEDKGGCMRSDKGPWNDPEVLKIAINREAKCSPISEDEHKHVDQGRSTSGFESLERIKKKTDEDNVYEKQIATIDKSMDMAWLAKTQKAENFPISKGLECYVRKGAPKKGDGLLVGGVMAFVMGIVAMVRLSKDVPRKLTEAALYGNSVCYEESTKSKQNQGQFAAPVSSSEYMLMVKRMAELEDKCMFLDLKPAHVESEKEEKLQAALNRVQVLEQELTETKKALEEALVSQKEILAYIEKKKKKKKLFFGF

Gene Information

Entrez Gene ID
829610
Gene Name
phosphatidylinositol transfer protein COW1
Gene Symbol
COW1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005794 IEA:UniProtKB-KW C Golgi apparatus
GO:0005886 IDA:UniProtKB C plasma membrane
GO:0035619 IDA:UniProtKB C root hair tip
GO:0008526 IGI:TAIR F phosphatidylinositol transporter activity
GO:0009932 IMP:TAIR P cell tip growth
GO:0015914 IGI:GOC P phospholipid transport
GO:0015031 IEA:UniProtKB-KW P protein transport
GO:0010053 IMP:TAIR P root epidermal cell differentiation
GO:0048768 IMP:UniProtKB P root hair cell tip growth
GO:0048767 IMP:UniProtKB P root hair elongation

Domain Information

InterPro Annotations

Accession Description
IPR001251 CRAL-TRIO domain
IPR011074 CRAL/TRIO, N-terminal domain
IPR001071 Cellular retinaldehyde binding/alpha-tocopherol transport

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol transfer protein COW1
Protein Entry
SFH1_ARATH
UniProt ID
F4JLE5
Species
Arabidopsis

Comments

Comment Type Description
Disruption Phenotype Exhibits short branched root hairs. {ECO:0000269|PubMed:15546352, ECO:0000269|PubMed:15728190, ECO:0000269|PubMed:23456248, ECO:0000269|PubMed:9390433}.
Function Required for transport of secretory proteins from the Golgi complex (By similarity). Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Plays a role in root hair tip elongation as a key regulator of polarized membrane trafficking. May promote the PtdIns(4,5)P2 synthesis and organization in root hair membrane. {ECO:0000250, ECO:0000269|PubMed:15546352, ECO:0000269|PubMed:15728190, ECO:0000269|PubMed:17335879, ECO:0000269|PubMed:23456248, ECO:0000269|PubMed:9390433}.
Sequence Caution Sequence=CAA18837.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAB80175.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=CAE82296.1; Type=Frameshift; Positions=551; Evidence={ECO:0000305}; Sequence=CAE82297.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the SFH family. {ECO:0000305}.
Similarity Contains 1 CRAL-TRIO domain. {ECO:0000255|PROSITE- ProRule:PRU00056}.
Subcellular Location Golgi apparatus membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}. Cell membrane {ECO:0000269|PubMed:15728190}; Peripheral membrane protein {ECO:0000269|PubMed:15728190}.
Tissue Specificity Predominantly expressed in roots. Detected solely in root trichoblast cell files engaged in root hair growth, hydathodes, shoot apical meristem, and apical cells of the root cap. {ECO:0000269|PubMed:15546352, ECO:0000269|PubMed:15728190}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010813 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
79497100 RefSeq NP_195184 554 phosphatidylinositol transfer protein COW1

Identical Sequences to LMP010813 proteins

Reference Database Accession Length Protein Name
GI:79497100 GenBank AEE86395.1 554 phosphatidylinositol transfer protein COW1 [Arabidopsis thaliana]
GI:79497100 SwissProt F4JLE5.1 554 RecName: Full=Phosphatidylinositol/phosphatidylcholine transfer protein SFH1; AltName: Full=Phosphatidylinositol transfer protein 1; Short=AtPITP1; AltName: Full=Protein CAN OF WORMS1; AltName: Full=Protein SEC FOURTEEN HOMOLOGS 1; Short=AtSFH1; AltName: Full=Protein SHORT ROOT HAIR 1 [Arabidopsis thaliana]

Related Sequences to LMP010813 proteins

Reference Database Accession Length Protein Name
GI:79497100 EMBL CAA18837.1 560 putative protein [Arabidopsis thaliana]
GI:79497100 EMBL CAB80175.1 560 putative protein [Arabidopsis thaliana]
GI:79497100 EMBL CAE82296.1 557 can of worms 1 protein [Arabidopsis thaliana]
GI:79497100 EMBL CAE82297.1 557 can of worms 1 [Arabidopsis thaliana]
GI:79497100 GenBank EFH45396.1 554 predicted protein [Arabidopsis lyrata subsp. lyrata]
GI:79497100 RefSeq XP_002869137.1 554 predicted protein [Arabidopsis lyrata subsp. lyrata]