Gene/Proteome Database (LMPD)

LMPD ID
LMP010646
Gene ID
844314
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
NADP-malic enzyme 4
Gene Symbol
NADP-ME4
Synonyms
Arabidopsis thaliana NADP-malic enzyme 4; ATNADP-ME4; F19K16.27; F19K16_27; NADP-malic enzyme 4; NADP-ME4
Alternate Names
NADP-malic enzyme 4
Chromosome
1
EC Number
1.1.1.40
Summary
The malic enzyme (EC 1.1.1.40) encoded by AtNADP-ME4 is localized to chloroplasts. The gene is expressed throughout the whole plant and during embryogenesis and germination. A possible involvement in the fatty acid biosynthesis has been proposed.
Orthologs
View orthologs and multiple alignments for NADP-ME4

Proteins

NADP-malic enzyme 4
Refseq ID NP_178093
Protein GI 15219980
UniProt ID Q9CA83
mRNA ID NM_106624
Length 646
RefSeq Status REVIEWED
MISLTPSLFLNKTVVPGCSTRLSLRQPRTIITPPASLRVFSSLGSNQDPTGSVLIETTATSSSSLETSAADIVPKSTVSGGVQDVYGEDAATEDMPITPWSLSVASGYTLLRDPHHNKGLAFSHRERDAHYLRGLLPPTVISQDLQVKKIMHTLRQYQVPLQKYMAMMDLQETNERLFYKLLIDHVEELLPVIYTPTVGEACQKYGSIFLRPQGLFISLKEKGKIHEVLRNWPEKNIQVIVVTDGERILGLGDLGCQGMGIPVGKLSLYTALGGVRPSACLPVTIDVGTNNEKLLNDEFYIGLRQRRATGEEYSELMHEFMTAVKQNYGEKVVIQFEDFANHNAFDLLAKYGTTHLVFNDDIQGTASVVLAGLIAALRFVGGSLSDHRFLFLGAGEAGTGIAELIALEISKKSHIPLEEARKNIWLVDSKGLIVSSRKESIQHFKKPWAHDHEPIRELVDAVKAIKPTVLIGTSGVGQTFTQDVVETMAKLNEKPIILSLSNPTSQSECTAEEAYTWSQGRAIFASGSPFAPVEYEGKTFVPGQANNAYIFPGFGLGLIMSGTIRVHDDMLLAASEALAEELMEEHYEKGMIYPPFRNIRKISARIAAKVAAKAYELGLATRLPQPKELEQCAESSMYSPSYRSYR

Gene Information

Entrez Gene ID
844314
Gene Name
NADP-malic enzyme 4
Gene Symbol
NADP-ME4
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IDA:TAIR C chloroplast
GO:0051287 IEA:InterPro F NAD binding
GO:0050897 IDA:TAIR F cobalt ion binding
GO:0004471 IEA:InterPro F malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0004473 IDA:TAIR F malate dehydrogenase (decarboxylating) (NADP+) activity
GO:0008948 IEA:UniProtKB-EC F oxaloacetate decarboxylase activity
GO:0042803 IDA:TAIR F protein homodimerization activity
GO:0008270 IDA:TAIR F zinc ion binding
GO:0006633 TAS:TAIR P fatty acid biosynthetic process
GO:0006108 IDA:TAIR P malate metabolic process
GO:0051289 IDA:TAIR P protein homotetramerization

KEGG Pathway Links

KEGG Pathway ID Description
ath_M00172 C4-dicarboxylic acid cycle, NADP - malic enzyme type
ath_M00169 CAM (Crassulacean acid metabolism), light
ath01200 Carbon metabolism
ath00620 Pyruvate metabolism

Domain Information

InterPro Annotations

Accession Description
IPR012301 Malic enzyme, N-terminal domain
IPR012302 Malic enzyme, NAD-binding
IPR015884 Malic enzyme, conserved site
IPR001891 Malic oxidoreductase
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
NADP-malic enzyme 4
Protein Entry
MAOP4_ARATH
UniProt ID
Q9CA83
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=10.2 uM for NADP (at pH 7.5) {ECO:0000269|PubMed:16113210}; KM=0.23 mM for malate (at pH 7.5) {ECO:0000269|PubMed:16113210}; Note=kcat is 151.3 sec(-1) with NADP as substrate (at pH 7.5).;
Catalytic Activity (S)-malate + NADP(+) = pyruvate + CO(2) + NADPH. {ECO:0000269|PubMed:16113210}.
Catalytic Activity Oxaloacetate = pyruvate + CO(2). {ECO:0000269|PubMed:16113210}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Divalent metal cations. Prefers magnesium or manganese. {ECO:0000250};
Developmental Stage During embryogenesis, present in the endosperm and the embryo at all developmental stages, including the seed attachment point and the integuments. Also detected in siliques. During germination, first confined to the radicle to later approaches the meristematic region. Finally present in the whole root. Expressed in cotyledons and primary leaves. In opened flowers, present in sepals, stigma, filaments, and pollen. {ECO:0000269|PubMed:16113210}.
Function The chloroplastic ME isoform decarboxylates malate shuttled from neighboring mesophyll cells. The CO(2) released is then refixed by ribulose-bisphosphate carboxylase. This pathway eliminates the photorespiratory loss of CO(2) that occurs in most plants (By similarity). {ECO:0000250}.
Pathway Photosynthesis; C3 acid pathway.
Sequence Caution Sequence=AAF68116.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; Sequence=BAD94826.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Similarity Belongs to the malic enzymes family. {ECO:0000305}.
Subcellular Location Plastid, chloroplast {ECO:0000269|PubMed:16113210, ECO:0000269|PubMed:21841088}.
Subunit Homodimer and homotetramer. {ECO:0000269|PubMed:16113210}.
Tissue Specificity Expressed in leaves, stems, flowers and roots, mainly in vascular system. In roots, present in the stele, including the vascular tissue and the pericycle, mainly at emerging lateral roots and at root tips. {ECO:0000269|PubMed:16113210}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010646 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15219980 RefSeq NP_178093 646 NADP-malic enzyme 4

Identical Sequences to LMP010646 proteins

Reference Database Accession Length Protein Name
GI:15219980 GenBank AAG52235.1 646 putative malate oxidoreductase; 93001-96525 [Arabidopsis thaliana]
GI:15219980 GenBank AAL31209.1 646 At1g79750/F19K16_27 [Arabidopsis thaliana]
GI:15219980 GenBank AAM98328.1 646 At1g79750/F19K16_27 [Arabidopsis thaliana]
GI:15219980 GenBank AEE36294.1 646 NADP-malic enzyme 4 [Arabidopsis thaliana]
GI:15219980 SwissProt Q9CA83.1 646 RecName: Full=NADP-dependent malic enzyme 4, chloroplastic; Short=AtNADP-ME4; Short=NADP-malic enzyme 4; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010646 proteins

Reference Database Accession Length Protein Name
GI:15219980 GenBank EFH64119.1 646 NADP-malic enzyme 4 [Arabidopsis lyrata subsp. lyrata]
GI:15219980 GenBank EOA34643.1 645 hypothetical protein CARUB_v10022207mg [Capsella rubella]
GI:15219980 RefSeq XP_002887860.1 646 NADP-malic enzyme 4 [Arabidopsis lyrata subsp. lyrata]
GI:15219980 RefSeq XP_006301745.1 645 hypothetical protein CARUB_v10022207mg [Capsella rubella]
GI:15219980 RefSeq XP_010417798.1 654 PREDICTED: NADP-dependent malic enzyme 4, chloroplastic [Camelina sativa]
GI:15219980 RefSeq XP_010473034.1 653 PREDICTED: NADP-dependent malic enzyme 4, chloroplastic-like [Camelina sativa]