Gene/Proteome Database (LMPD)

LMPD ID
LMP010558
Gene ID
830740
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
lipoyl synthase
Gene Symbol
AT5G08415
Alternate Names
lipoyl synthase
Chromosome
5
EC Number
2.8.1.8

Proteins

lipoyl synthase
Refseq ID NP_568196
Protein GI 18415808
UniProt ID Q8LEE8
mRNA ID NM_120926
Length 394
RefSeq Status REVIEWED
MMHHCSITKPTFSISISTQKLHHHSSKFLNLGFRIRCESGDVSSPLRTKAVSLSSEMEDSSSLKKSLMELEGKKSEPYPGGMPKMGPFTGRDPNVKKPAWLRQKAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGDTCTRGCRFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIPDGGSGHFAQTVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQRLVRDPRAGYEQSMSVLKHAKISKPGMITKTSIMLGLGETDEELKEAMADLRAIDVDILTLGQYLQPTPLHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGELFVKTMVKESYSKSLS

Gene Information

Entrez Gene ID
830740
Gene Name
lipoyl synthase
Gene Symbol
AT5G08415
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IEA:UniProtKB-HAMAP C chloroplast
GO:0051539 IEA:UniProtKB-HAMAP F 4 iron, 4 sulfur cluster binding
GO:0016992 IEA:UniProtKB-HAMAP F lipoate synthase activity
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0009249 IEA:UniProtKB-HAMAP P protein lipoylation

KEGG Pathway Links

KEGG Pathway ID Description
ath00785 Lipoic acid metabolism
ko00785 Lipoic acid metabolism
ath01100 Metabolic pathways

Domain Information

InterPro Annotations

Accession Description
IPR013785 Aldolase-type TIM barrel
IPR006638 Elongator protein 3/MiaB/NifB
IPR003698 Lipoyl synthase
IPR027526 Lipoyl synthase, chloroplastic
IPR007197 Radical SAM

UniProt Annotations

Entry Information

Gene Name
lipoyl synthase
Protein Entry
LISC_ARATH
UniProt ID
Q8LEE8
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity Protein N(6)-(octanoyl)lysine + 2 sulfur- (sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)- (lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'- deoxyadenosine. {ECO:0000255|HAMAP-Rule:MF_03129}.
Cofactor Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000255|HAMAP-Rule:MF_03129}; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L- methionine. {ECO:0000255|HAMAP-Rule:MF_03129};
Function Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. {ECO:0000255|HAMAP-Rule:MF_03129, ECO:0000269|PubMed:12062419}.
Pathway Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2. {ECO:0000255|HAMAP-Rule:MF_03129}.
Sequence Caution Sequence=AAM62684.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=CAC08341.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Belongs to the radical SAM superfamily. Lipoyl synthase family. {ECO:0000255|HAMAP-Rule:MF_03129}.
Subcellular Location Plastid, chloroplast {ECO:0000255|HAMAP- Rule:MF_03129, ECO:0000269|PubMed:12062419}.
Tissue Specificity Expressed in roots, leaves and flowers. {ECO:0000269|PubMed:12062419}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010558 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18415808 RefSeq NP_568196 394 lipoyl synthase

Identical Sequences to LMP010558 proteins

Reference Database Accession Length Protein Name
GI:18415808 DBBJ BAB91180.1 394 lipoic acid synthase [Arabidopsis thaliana]
GI:18415808 EMBL CBM36052.1 394 unnamed protein product [Arabidopsis thaliana]
GI:18415808 GenBank ABF58967.1 394 At5g08415 [Arabidopsis thaliana]
GI:18415808 GenBank ACX19713.1 394 Sequence 47990 from patent US 7569389
GI:18415808 gnl TAIR 394 lipoyl synthase [Arabidopsis thaliana]
GI:18415808 SwissProt Q8LEE8.2 394 RecName: Full=Lipoyl synthase, chloroplastic; AltName: Full=Lipoate synthase; Short=LS; Short=Lip-syn; AltName: Full=Lipoate synthase, plastidial; Short=LIP1p; AltName: Full=Lipoic acid synthase; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010558 proteins

Reference Database Accession Length Protein Name
GI:18415808 EMBL CBM36178.1 393 unnamed protein product [Arabidopsis thaliana]
GI:18415808 EMBL CBN72484.1 393 unnamed protein product [Arabidopsis thaliana]
GI:18415808 GenBank AAM62684.1 393 lipoic acid synthase-like protein [Arabidopsis thaliana]
GI:18415808 GenBank ACX19714.1 393 Sequence 47991 from patent US 7569389
GI:18415808 GenBank EOA22984.1 395 hypothetical protein CARUB_v10003725mg [Capsella rubella]
GI:18415808 RefSeq XP_006290086.1 395 hypothetical protein CARUB_v10003725mg [Capsella rubella]