LIPID MAPS

LMPD ID

LMP010140

Gene ID

841221

Species

Arabidopsis thaliana (Arabidopsis)

Gene Name

dihydrolipoyl dehydrogenase 1

Gene Symbol

mtLPD1

Synonyms

mitochondrial lipoamide dehydrogenase 1; mtLPD1; T2J15.6

Alternate Names

dihydrolipoyl dehydrogenase 1

Chromosome

1

EC Number

1.8.1.4

Summary

Encodes a mitochondrial lipoamide dehydrogenase whose expression is induced by light.

Orthologs

View orthologs and multiple alignments for mtLPD1

dihydrolipoyl dehydrogenase 1
Refseq ID	NP_175237
Protein GI	15221044
UniProt ID	Q9M5K3
mRNA ID	NM_103699
Length	507
RefSeq Status	REVIEWED
MAMASLARRKAYFLTRNLSNSPTDALRFSFSLSRGFASSGSDENDVVIIGGGPGGYVAAIKASQLGLKTTCIEKRGALGGTCLNVGCIPSKALLHSSHMYHEAKHSFANHGIKVSSVEVDLPAMLAQKDNAVKNLTRGIEGLFKKNKVTYVKGYGKFISPNEVSVETIDGGNTIVKGKHIIVATGSDVKSLPGITIDEKKIVSSTGALSLSEVPKKLIVIGAGYIGLEMGSVWGRLGSEVTVVEFAGDIVPSMDGEIRKQFQRSLEKQKMKFMLKTKVVSVDSSSDGVKLTVEPAEGGEQSILEADVVLVSAGRTPFTSGLDLEKIGVETDKAGRILVNDRFLSNVPGVYAIGDVIPGPMLAHKAEEDGVACVEFIAGKHGHVDYDKVPGVVYTHPEVASVGKTEEQLKKEGVSYRVGKFPFMANSRAKAIDNAEGLVKILADKETDKILGVHIMAPNAGELIHEAVLAINYDASSEDIARVCHAHPTMSEALKEAAMATYDKPIHI

dihydrolipoyl dehydrogenase 1
Refseq ID	NP_849782
Protein GI	30694221
UniProt ID	Q9M5K3
mRNA ID	NM_179451
Length	507
RefSeq Status	REVIEWED
Protein sequence is identical to GI:15221044 (mRNA isoform)

Entrez Gene ID

841221

Gene Name

dihydrolipoyl dehydrogenase 1

Gene Symbol

mtLPD1

Species

Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0048046	IDA:TAIR	C	apoplast
GO:0005747	IDA:TAIR	C	mitochondrial respiratory chain complex I
GO:0005739	IDA:TAIR	C	mitochondrion
GO:0005524	IDA:TAIR	F	ATP binding
GO:0050897	IDA:TAIR	F	cobalt ion binding
GO:0005507	IDA:TAIR	F	copper ion binding
GO:0004148	IEA:UniProtKB-EC	F	dihydrolipoyl dehydrogenase activity
GO:0050660	IEA:InterPro	F	flavin adenine dinucleotide binding
GO:0008270	IDA:TAIR	F	zinc ion binding
GO:0045454	IEA:InterPro	P	cell redox homeostasis
GO:0046686	IEP:TAIR	P	response to cadmium ion

KEGG Pathway Links

KEGG Pathway ID	Description
ath01200	Carbon metabolism
ath00020	Citrate cycle (TCA cycle)
ath00260	Glycine, serine and threonine metabolism
ath00010	Glycolysis / Gluconeogenesis

InterPro Annotations

Accession	Description
IPR006258	Dihydrolipoamide dehydrogenase
IPR013027	FAD-dependent pyridine nucleotide-disulphide oxidoreductase
IPR016156	FAD/NAD-linked reductase, dimerisation domain
IPR023753	Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain
IPR001327	Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain
IPR012999	Pyridine nucleotide-disulphide oxidoreductase, class I, active site
IPR004099	Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain

Entry Information

Gene Name

dihydrolipoyl dehydrogenase 1

Protein Entry

DLDH1_ARATH

UniProt ID

Q9M5K3

Species

Arabidopsis

Comments

Comment Type	Description
Catalytic Activity	Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH.
Cofactor	Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250}; Note=Binds 1 FAD per subunit. {ECO:0000250};
Function	Lipoamide dehydrogenase is a component of the glycine decarboxylase (GDC) or glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. LPD1 is probably the protein most often associated with the glycine decarboxylase complex while LPD2 is probably incorporated into alpha-ketoacid dehydrogenase complexes. {ECO:0000269\|PubMed:11598235}.
Induction	Up-regulated by light. {ECO:0000269\|PubMed:11598235}.
Miscellaneous	The active site is a redox-active disulfide bond.
Ptm	S-nytrosylated at unknown positions.
Sequence Caution	Sequence=AAF79529.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Similarity	Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. {ECO:0000305}.
Subcellular Location	Mitochondrion matrix {ECO:0000269\|PubMed:14671022}.
Subunit	Homodimer (By similarity). Part of both the glycine cleavage system composed of four proteins: P, T, L and H and of the pyruvate dehydrogenase complex containing multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). {ECO:0000250}.
Tissue Specificity	Preferentially expressed in leaves, flowers and siliques and at a lower level in roots and stems. {ECO:0000269\|PubMed:11598235}.

Unique RefSeq proteins for LMP010140 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
15221044	RefSeq	NP_175237	507	dihydrolipoyl dehydrogenase 1

Identical Sequences to LMP010140 proteins

Reference	Database	Accession	Length	Protein Name
GI:15221044	EMBL	CAJ00711.1	507	unnamed protein product [Arabidopsis thaliana]
GI:15221044	GenBank	AAF34795.3	507	lipoamide dehydrogenase precursor [Arabidopsis thaliana]
GI:15221044	GenBank	AEE32239.1	507	dihydrolipoyl dehydrogenase 1 [Arabidopsis thaliana]
GI:15221044	GenBank	AEE32240.1	507	dihydrolipoyl dehydrogenase 1 [Arabidopsis thaliana]
GI:15221044	RefSeq	NP_849782.1	507	dihydrolipoyl dehydrogenase 1 [Arabidopsis thaliana]
GI:15221044	SwissProt	Q9M5K3.2	507	RecName: Full=Dihydrolipoyl dehydrogenase 1, mitochondrial; Short=AtmLPD1; Short=mtLPD1; AltName: Full=Dihydrolipoamide dehydrogenase 1; AltName: Full=Glycine cleavage system L protein 1; AltName: Full=Pyruvate dehydrogenase complex E3 subunit 1; Short=E3-1; Short=PDC-E3 1; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010140 proteins

Reference	Database	Accession	Length	Protein Name
GI:15221044	GenBank	AAF79529.1	505	F21D18.28 [Arabidopsis thaliana]
GI:15221044	GenBank	EFH70350.1	505	F21D18.28 [Arabidopsis lyrata subsp. lyrata]
GI:15221044	GenBank	AGD35810.1	505	Sequence 33266 from patent US 8343764
GI:15221044	RefSeq	XP_002894091.1	505	F21D18.28 [Arabidopsis lyrata subsp. lyrata]
GI:15221044	RefSeq	XP_010500315.1	507	PREDICTED: dihydrolipoyl dehydrogenase 1, mitochondrial [Camelina sativa]
GI:15221044	RefSeq	XP_010461603.1	507	PREDICTED: dihydrolipoyl dehydrogenase 1, mitochondrial [Camelina sativa]