Gene/Proteome Database (LMPD)

LMPD ID
LMP010139
Gene ID
820951
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
lipoamide dehydrogenase 1
Gene Symbol
LPD1
Synonyms
LIPOAMIDE DEHYDROGENASE; lipoamide dehydrogenase 1; LPD1; ptlpd1
Alternate Names
lipoamide dehydrogenase 1
Chromosome
3
EC Number
1.8.1.4
Summary
encodes a plastid lipoamide dehydrogenase, subunit of the pyruvate dehydrogenase complex which provides acetyl-CoA for de novo fatty acid biosynthesis. The gene is highly expressed in developing seeds.
Orthologs
View orthologs and multiple alignments for LPD1

Proteins

lipoamide dehydrogenase 1
Refseq ID NP_001078165
Protein GI 145332599
UniProt ID A8MS68
mRNA ID NM_001084696
Length 623
RefSeq Status REVIEWED
MQSAMALSFSQTSFTRPNHVLGSSGSVFSTPRSLRFCGLRREAFGFSTSNQLAIRSNRIQFLSRKSFQVSASASSNGNGAPPKSFDYDLIIIGAGVGGHGAALHAVEKGLKTAIIEGDVVGGTCVNRGCVPSKALLAVSGRMRELQNEHHMKSFGLQVSAAGYDRQGVADHANNLATKIRNNLTNSMKAIGVDILTGFGSVLGPQKVKYGKDNIITAKDIIIATGSVPFVPKGIEVDGKTVITSDHALKLESVPEWIAIVGSGYIGLEFSDVYTALGSEVTFIEALDQLMPGFDPEISKLAQRVLINPRKIDYHTGVFASKITPARDGKPVLIELIDAKTKEPKDTLEVDAALIATGRAPFTNGLGLENVNVVTQRGFIPVDERMRVIDGKGTLVPNLYCIGDANGKLMLAHAASAQGISVVEQVSGRDHVLNHLSIPAACFTHPEISMVGLTEPQAKEKGEKEGFKVSVVKTSFKANTKALAENEGEGIAKMIYRPDNGEILGVHIFGLHAADLIHEASNAIALGTRIQDIKLAVHAHPTLSEVLDELFKAAKVESHATTRTGDAKIKLNTNQEDRKGRRRGGDDEKQPSVSKDLKDISTRPSSFFENISVGVLSLLSLIFV
lipoamide dehydrogenase 1
Refseq ID NP_566562
Protein GI 18401311
UniProt ID A8MS68
mRNA ID NM_112571
Length 570
RefSeq Status REVIEWED
MQSAMALSFSQTSFTRPNHVLGSSGSVFSTPRSLRFCGLRREAFGFSTSNQLAIRSNRIQFLSRKSFQVSASASSNGNGAPPKSFDYDLIIIGAGVGGHGAALHAVEKGLKTAIIEGDVVGGTCVNRGCVPSKALLAVSGRMRELQNEHHMKSFGLQVSAAGYDRQGVADHANNLATKIRNNLTNSMKAIGVDILTGFGSVLGPQKVKYGKDNIITAKDIIIATGSVPFVPKGIEVDGKTVITSDHALKLESVPEWIAIVGSGYIGLEFSDVYTALGSEVTFIEALDQLMPGFDPEISKLAQRVLINPRKIDYHTGVFASKITPARDGKPVLIELIDAKTKEPKDTLEVDAALIATGRAPFTNGLGLENVNVVTQRGFIPVDERMRVIDGKGTLVPNLYCIGDANGKLMLAHAASAQGISVVEQVSGRDHVLNHLSIPAACFTHPEISMVGLTEPQAKEKGEKEGFKVSVVKTSFKANTKALAENEGEGIAKMIYRPDNGEILGVHIFGLHAADLIHEASNAIALGTRIQDIKLAVHAHPTLSEVLDELFKAAKVESHATTRTVSEKVVV

Gene Information

Entrez Gene ID
820951
Gene Name
lipoamide dehydrogenase 1
Gene Symbol
LPD1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IDA:TAIR C chloroplast
GO:0009941 IDA:TAIR C chloroplast envelope
GO:0009570 IDA:TAIR C chloroplast stroma
GO:0005730 IDA:TAIR C nucleolus
GO:0004148 ISS:TAIR F dihydrolipoyl dehydrogenase activity
GO:0050660 IEA:InterPro F flavin adenine dinucleotide binding
GO:0006086 TAS:TAIR P acetyl-CoA biosynthetic process from pyruvate
GO:0045454 IEA:InterPro P cell redox homeostasis
GO:0046685 IMP:UniProtKB P response to arsenic-containing substance

KEGG Pathway Links

KEGG Pathway ID Description
ath01200 Carbon metabolism
ath00020 Citrate cycle (TCA cycle)
ath00260 Glycine, serine and threonine metabolism
ath00010 Glycolysis / Gluconeogenesis

Domain Information

InterPro Annotations

Accession Description
IPR006258 Dihydrolipoamide dehydrogenase
IPR013027 FAD-dependent pyridine nucleotide-disulphide oxidoreductase
IPR016156 FAD/NAD-linked reductase, dimerisation domain
IPR023753 Pyridine nucleotide-disulphide oxidoreductase, FAD/NAD(P)-binding domain
IPR001327 Pyridine nucleotide-disulphide oxidoreductase, NAD-binding domain
IPR012999 Pyridine nucleotide-disulphide oxidoreductase, class I, active site
IPR004099 Pyridine nucleotide-disulphide oxidoreductase, dimerisation domain

UniProt Annotations

Entry Information

Gene Name
lipoamide dehydrogenase 1
Protein Entry
PLPD1_ARATH
UniProt ID
A8MS68
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=A8MS68-1; Sequence=Displayed; Name=2; IsoId=A8MS68-2; Sequence=VSP_047927, VSP_047928;
Catalytic Activity Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH.
Cofactor Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000250}; Note=Binds 1 FAD per subunit. {ECO:0000250};
Disruption Phenotype Arsenate hypersensitivity. {ECO:0000269|PubMed:20488895}.
Function Lipoamide dehydrogenase is a component of the plastidial pyruvate dehydrogenase complex (PDC). {ECO:0000269|PubMed:11056213}.
Miscellaneous The active site is a redox-active disulfide bond. {ECO:0000250}.
Similarity Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. {ECO:0000305}.
Subcellular Location Plastid, chloroplast stroma {ECO:0000269|PubMed:11056213}.
Subunit Homodimer (By similarity). Part of the plastidial pyruvate dehydrogenase complex (PDC) containing multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). {ECO:0000250}.
Tissue Specificity Expressed mainly in flower buds and immature siliques, and to a lesser extend in flowers. {ECO:0000269|PubMed:11056213}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010139 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
145332599 RefSeq NP_001078165 623 lipoamide dehydrogenase 1
18401311 RefSeq NP_566562 570 lipoamide dehydrogenase 1

Identical Sequences to LMP010139 proteins

Reference Database Accession Length Protein Name
GI:18401311 GenBank ACX18646.1 570 Sequence 46549 from patent US 7569389
GI:18401311 GenBank ADR86633.1 570 Sequence 367 from patent US 7737329
GI:18401311 GenBank AEE75887.1 570 lipoamide dehydrogenase 1 [Arabidopsis thaliana]
GI:145332599 GenBank AEE75888.1 623 lipoamide dehydrogenase 1 [Arabidopsis thaliana]
GI:18401311 GenBank AEF79756.1 570 Sequence 367 from patent US 7943822
GI:145332599 GenBank AFX58135.1 623 Sequence 82 from patent US 8309323
GI:18401311 GenBank AGB67507.1 570 Sequence 367 from patent US 8288616
GI:18401311 GenBank AHL66969.1 570 Sequence 367 from patent US 8658858
GI:145332599 SwissProt A8MS68.1 623 RecName: Full=Dihydrolipoyl dehydrogenase 1, chloroplastic; Short=ptLPD1; AltName: Full=Dihydrolipoamide dehydrogenase 1; AltName: Full=Protein LIPOAMIDE DEHYDROGENASE 1; AltName: Full=Pyruvate dehydrogenase complex E3 subunit 1; Short=E3-1; Short=PDC-E3 1; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010139 proteins

Reference Database Accession Length Protein Name
GI:18401311 DBBJ BAH19701.1 623 AT3G16950 [Arabidopsis thaliana]
GI:145332599 DBBJ BAH19701.1 623 AT3G16950 [Arabidopsis thaliana]
GI:145332599 GenBank EFH61438.1 615 hypothetical protein ARALYDRAFT_479163 [Arabidopsis lyrata subsp. lyrata]
GI:145332599 GenBank AEE75887.1 570 lipoamide dehydrogenase 1 [Arabidopsis thaliana]
GI:18401311 GenBank AEE75888.1 623 lipoamide dehydrogenase 1 [Arabidopsis thaliana]
GI:18401311 GenBank AFX58135.1 623 Sequence 82 from patent US 8309323
GI:18401311 RefSeq NP_001078165.1 623 lipoamide dehydrogenase 1 [Arabidopsis thaliana]
GI:145332599 RefSeq XP_002885179.1 615 hypothetical protein ARALYDRAFT_479163 [Arabidopsis lyrata subsp. lyrata]
GI:18401311 RefSeq XP_002885179.1 615 hypothetical protein ARALYDRAFT_479163 [Arabidopsis lyrata subsp. lyrata]
GI:145332599 RefSeq XP_010504843.1 622 PREDICTED: dihydrolipoyl dehydrogenase 1, chloroplastic-like isoform X2 [Camelina sativa]
GI:145332599 RefSeq XP_010465725.1 615 PREDICTED: dihydrolipoyl dehydrogenase 1, chloroplastic isoform X2 [Camelina sativa]
GI:18401311 SwissProt A8MS68.1 623 RecName: Full=Dihydrolipoyl dehydrogenase 1, chloroplastic; Short=ptLPD1; AltName: Full=Dihydrolipoamide dehydrogenase 1; AltName: Full=Protein LIPOAMIDE DEHYDROGENASE 1; AltName: Full=Pyruvate dehydrogenase complex E3 subunit 1; Short=E3-1; Short=PDC-E3 1; Flags: Precursor [Arabidopsis thaliana]