Gene/Proteome Database (LMPD)

LMPD ID
LMP010095
Gene ID
823642
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
sulfotransferase 5
Gene Symbol
AT3G45070
Alternate Names
sulfotransferase 5
Chromosome
3
EC Number
2.8.2.-

Proteins

sulfotransferase 5
Refseq ID NP_190093
Protein GI 15230602
UniProt ID Q9M1V2
mRNA ID NM_114376
Length 323
RefSeq Status REVIEWED
MEMNLRIEDLNEETKTLISSLPSDKDFTGKTICKYQGCWYTHNVLQAVLNFQKSFKPQDTDIIVASFPKCGTTWLKALTFALLHRSKQPSHDDDHPLLSNNPHVLVPYFEIDLYLRSENPDLTKFSSSPRLFSTHVPSHTLQEGLKGSTCKIVYISRNVKDTLVSYWHFFTKKQTDEKIISSFEDTFEMFCRGVSIFGPFWDHVLSYWRGSLEDPNHVLFMKFEEMKAEPRDQIKKFAEFLGCPFTKEEEESGSVDEIIDLCSLRNLSSLEINKTGKLNSGRENKMFFRKGEVGDWKNYLTPEMENKIDMIIQEKLQNSGLKF

Gene Information

Entrez Gene ID
823642
Gene Name
sulfotransferase 5
Gene Symbol
AT3G45070
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:1990135 IDA:TAIR F flavonoid sulfotransferase activity
GO:0008146 IDA:UniProtKB F sulfotransferase activity
GO:0009812 IDA:UniProtKB P flavonoid metabolic process

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6546 brassinosteroids inactivation

REACTOME Pathway Links

REACTOME Pathway ID Description
6253661 Biological oxidations
6254007 Cytosolic sulfonation of small molecules
6253660 Phase II conjugation

Domain Information

InterPro Annotations

Accession Description
IPR027417 P-loop containing nucleoside triphosphate hydrolase
IPR000863 Sulfotransferase domain

UniProt Annotations

Entry Information

Gene Name
sulfotransferase 5
Protein Entry
SOT5_ARATH
UniProt ID
Q9M1V2
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=2 uM for kaempferol 3-sulfate {ECO:0000269|PubMed:17095238}; KM=6 uM for isorhamnetin {ECO:0000269|PubMed:17095238}; KM=3.2 uM for kaempferol {ECO:0000269|PubMed:17095238}; KM=11 uM for galangin {ECO:0000269|PubMed:17095238}; KM=1.5 uM for quercetin {ECO:0000269|PubMed:17095238}; KM=5.1 uM for apigenin 4'-sulfate {ECO:0000269|PubMed:17095238}; KM=51 uM for apigenin {ECO:0000269|PubMed:17095238}; KM=40.6 uM for chrysin {ECO:0000269|PubMed:17095238}; KM=26 uM for 7-hydroxyflavone {ECO:0000269|PubMed:17095238}; KM=1 uM for 3'-phospho-5'-adenylyl sulfate {ECO:0000269|PubMed:17095238}; Vmax=286 pmol/sec/mg enzyme with kaempferol 3-sulfate as substrate {ECO:0000269|PubMed:17095238}; Vmax=232 pmol/sec/mg enzyme with isorhamnetin as substrate {ECO:0000269|PubMed:17095238}; Vmax=116 pmol/sec/mg enzyme with kaempferol as substrate {ECO:0000269|PubMed:17095238}; Vmax=270 pmol/sec/mg enzyme with galangin as substrate {ECO:0000269|PubMed:17095238}; Vmax=24 pmol/sec/mg enzyme with quercetin as substrate {ECO:0000269|PubMed:17095238}; Vmax=45 pmol/sec/mg enzyme with apigenin 4'-sulfate as substrate {ECO:0000269|PubMed:17095238}; Vmax=370 pmol/sec/mg enzyme with apigenin as substrate {ECO:0000269|PubMed:17095238}; Vmax=156 pmol/sec/mg enzyme with chrysin as substrate {ECO:0000269|PubMed:17095238}; Vmax=91 pmol/sec/mg enzyme with 7-hydroxyflavone as substrate {ECO:0000269|PubMed:17095238}; pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:17095238};
Developmental Stage Expressed mostly in 5-day old seedlings, at developmental stage 1. {ECO:0000269|PubMed:17095238}.
Function Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to specifically catalyze the sulfate conjugation of flavones and flavonols. Strictly specific for the position 7. Substrate preference is kaempferol 3-sulfate > isorhamnetin > kaempferol. {ECO:0000269|PubMed:17095238}.
Induction Up-regulated by trans-zeatin, but not by cold, heat, hypoxia, salt stress, auxins, gibberellins, wounding, etiolation and salicylic acid or methyl jasmonate treatments. {ECO:0000269|PubMed:17095238}.
Similarity Belongs to the sulfotransferase 1 family. {ECO:0000305}.
Subcellular Location Cytoplasm {ECO:0000250}.
Tissue Specificity Expressed in inflorescence stems, roots and siliques. {ECO:0000269|PubMed:17095238}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010095 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15230602 RefSeq NP_190093 323 sulfotransferase 5

Identical Sequences to LMP010095 proteins

Reference Database Accession Length Protein Name
GI:15230602 GenBank AAS49096.1 323 At3g45070 [Arabidopsis thaliana]
GI:15230602 GenBank ACW86189.1 323 Sequence 2427 from patent US 7569389
GI:15230602 GenBank AEE77988.1 323 sulfotransferase 5 [Arabidopsis thaliana]
GI:15230602 GenBank AEQ45472.1 323 Sequence 54 from patent US 8034993
GI:15230602 GenBank AFO05571.1 323 Sequence 54 from patent US 8217225
GI:15230602 SwissProt Q9M1V2.1 323 RecName: Full=Cytosolic sulfotransferase 5; Short=AtSOT5; AltName: Full=Flavonoid 7-sulfotransferase 3a; Short=AtST3a [Arabidopsis thaliana]

Related Sequences to LMP010095 proteins

Reference Database Accession Length Protein Name
GI:15230602 GenBank AAT70444.1 329 At3g45080 [Arabidopsis thaliana]
GI:15230602 GenBank ACW86190.1 321 Sequence 2428 from patent US 7569389
GI:15230602 GenBank ACW86191.1 329 Sequence 2430 from patent US 7569389
GI:15230602 GenBank AEQ45473.1 329 Sequence 56 from patent US 8034993
GI:15230602 GenBank AFO05572.1 329 Sequence 56 from patent US 8217225
GI:15230602 RefSeq NP_190094.1 329 cytosolic sulfotransferase 6 [Arabidopsis thaliana]