Gene/Proteome Database (LMPD)

LMPD ID
LMP010069
Gene ID
818013
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
cytochrome P450 710A1
Gene Symbol
CYP710A1
Synonyms
cytochrome P450; F13P17.35; family 710; polypeptide 1; subfamily A
Alternate Names
cytochrome P450 710A1
Chromosome
2
EC Number
1.14.-.-
Summary
Encodes a protein with C22-sterol desaturase activity. The enzyme was shown to catalyze in the presence of NADPH the conversion of &beta;-sitosterol to stigmasterol, but not that of 24-<i>epi</i>-campesterol to brassicasterol (unlike CYP710A2).
Orthologs
View orthologs and multiple alignments for CYP710A1

Proteins

cytochrome P450 710A1
Refseq ID NP_180997
Protein GI 15226758
UniProt ID O64697
mRNA ID NM_129002
Length 495
RefSeq Status REVIEWED
MVFSVSIFASLAPYLISAFLLFLLVEQLSYLFKKRNIPGPFFVPPIIGNAVALVRDPTSFWDKQSSTANISGLSANYLIGKFIVYIRDTELSHQIFSNVRPDAFHLIGHPFGKKLFGDHNLIYMFGEDHKSVRRQLAPNFTPKALSTYSALQQLVILRHLRQWEGSTSGGSRPVSLRQLVRELNLETSQTVFVGPYLDKEAKNRFRTDYNLFNLGSMALPIDLPGFAFGEARRAVKRLGETLGICAGKSKARMAAGEEPACLIDFWMQAIVAENPQPPHSGDEEIGGLLFDFLFAAQDASTSSLLWAVTLLDSEPEVLNRVREEVAKIWSPESNALITVDQLAEMKYTRSVAREVIRYRPPATMVPHVAAIDFPLTETYTIPKGTIVFPSVFDSSFQGFTEPDRFDPDRFSETRQEDQVFKRNFLAFGWGPHQCVGQRYALNHLVLFIAMFSSLLDFKRLRSDGCDEIVYCPTISPKDGCTVFLSRRVAKYPNFS

Gene Information

Entrez Gene ID
818013
Gene Name
cytochrome P450 710A1
Gene Symbol
CYP710A1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0000249 IDA:TAIR F C-22 sterol desaturase activity
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0004497 IEA:UniProtKB-KW F monooxygenase activity
GO:0016126 IEA:UniProtKB-UniPathway P sterol biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01110 Biosynthesis of secondary metabolites
ath00100 Steroid biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-2541 plant sterol biosynthesis
PWY-2541 plant sterol biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450 710A1
Protein Entry
C7101_ARATH
UniProt ID
O64697
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=1 uM for beta-sisterol {ECO:0000269|PubMed:16531502};
Cofactor Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
Disruption Phenotype No visible phenotype under normal growth condition, but upon infection with P.syringae, no accumulation of stigmasterol and increased resistance to the pathogen. {ECO:0000269|PubMed:20444228}.
Function Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward beta- sitosterol and produces stigmasterol. {ECO:0000269|PubMed:16531502, ECO:0000269|PubMed:17909855, ECO:0000269|PubMed:20444228}.
Induction By infection with Pseudomonas syringae. {ECO:0000269|PubMed:20444228}.
Miscellaneous Plants overexpressing CYP710A2 show higher levels of stigmasterol and lower levels of beta-sitosterol than the wild- type.
Pathway Steroid biosynthesis; sterol biosynthesis.
Similarity Belongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular Location Membrane {ECO:0000305}; Single-pass membrane protein {ECO:0000305}.
Tissue Specificity Expressed in the vascular tissues of roots, shoots and leaves. Expressed in root tips and sepals. {ECO:0000269|PubMed:16531502}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010069 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15226758 RefSeq NP_180997 495 cytochrome P450 710A1

Identical Sequences to LMP010069 proteins

Reference Database Accession Length Protein Name
GI:15226758 EMBL CBU96638.1 495 unnamed protein product [Arabidopsis thaliana]
GI:15226758 GenBank AEC08982.1 495 cytochrome P450 710A1 [Arabidopsis thaliana]
GI:15226758 GenBank AFX28337.1 495 Sequence 9397 from patent US 8299318
GI:15226758 GenBank AFX49555.1 495 Sequence 51388 from patent US 8299318
GI:15226758 GenBank AGF14973.1 495 Sequence 14392 from patent US 8362325
GI:15226758 GenBank AGX64066.1 495 Sequence 28236 from patent US 8541208

Related Sequences to LMP010069 proteins

Reference Database Accession Length Protein Name
GI:15226758 GenBank EFH57598.1 497 CYP710A1 [Arabidopsis lyrata subsp. lyrata]
GI:15226758 GenBank EOA28522.1 498 hypothetical protein CARUB_v10024737mg [Capsella rubella]
GI:15226758 RefSeq XP_002881339.1 497 CYP710A1 [Arabidopsis lyrata subsp. lyrata]
GI:15226758 RefSeq XP_006295624.1 498 hypothetical protein CARUB_v10024737mg [Capsella rubella]
GI:15226758 RefSeq XP_010504911.1 497 PREDICTED: cytochrome P450 710A1-like [Camelina sativa]
GI:15226758 RefSeq XP_010509743.1 497 PREDICTED: cytochrome P450 710A1 [Camelina sativa]