LIPID MAPS

LMPD ID

LMP010013

Gene ID

824479

Species

Arabidopsis thaliana (Arabidopsis)

Gene Name

carotene epsilon-monooxygenase

Gene Symbol

LUT1

Synonyms

CYP97C1; CYTOCHROME P450 97C1; LUT1; LUTEIN DEFICIENT 1

Alternate Names

carotene epsilon-monooxygenase

Chromosome

3

EC Number

1.14.99.45

Summary

Lutein-deficient 1 (LUT1) required for lutein biosynthesis, member of the xanthophyll class of carotenoids. Involved in epsilon ring hydroxylation. Maps at 67.3 cM on chromosome 3.

Orthologs

View orthologs and multiple alignments for LUT1

carotene epsilon-monooxygenase
Refseq ID	NP_190881
Protein GI	42565881
UniProt ID	Q6TBX7
mRNA ID	NM_115173
Length	539
RefSeq Status	REVIEWED
MESSLFSPSSSSYSSLFTAKPTRLLSPKPKFTFSIRSSIEKPKPKLETNSSKSQSWVSPDWLTTLTRTLSSGKNDESGIPIANAKLDDVADLLGGALFLPLYKWMNEYGPIYRLAAGPRNFVIVSDPAIAKHVLRNYPKYAKGLVAEVSEFLFGSGFAIAEGPLWTARRRAVVPSLHRRYLSVIVERVFCKCAERLVEKLQPYAEDGSAVNMEAKFSQMTLDVIGLSLFNYNFDSLTTDSPVIEAVYTALKEAELRSTDLLPYWKIDALCKIVPRQVKAEKAVTLIRETVEDLIAKCKEIVEREGERINDEEYVNDADPSILRFLLASREEVSSVQLRDDLLSMLVAGHETTGSVLTWTLYLLSKNSSALRKAQEEVDRVLEGRNPAFEDIKELKYITRCINESMRLYPHPPVLIRRAQVPDILPGNYKVNTGQDIMISVYNIHRSSEVWEKAEEFLPERFDIDGAIPNETNTDFKFIPFSGGPRKCVGDQFALMEAIVALAVFLQRLNVELVPDQTISMTTGATIHTTNGLYMKVSQR

Entrez Gene ID

824479

Gene Name

carotene epsilon-monooxygenase

Gene Symbol

LUT1

Species

Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0009507	IDA:TAIR	C	chloroplast
GO:0009941	IDA:TAIR	C	chloroplast envelope
GO:0016020	IEA:UniProtKB-KW	C	membrane
GO:0020037	IEA:InterPro	F	heme binding
GO:0005506	IEA:InterPro	F	iron ion binding
GO:0009974	IMP:TAIR	F	zeinoxanthin epsilon hydroxylase activity
GO:0016117	IMP:TAIR	P	carotenoid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID	Description
ath01110	Biosynthesis of secondary metabolites
ath00906	Carotenoid biosynthesis
ath01100	Metabolic pathways

REACTOME Pathway Links

REACTOME Pathway ID	Description
6253809	Cytochrome P450 - arranged by substrate type

InterPro Annotations

Accession	Description
IPR001128	Cytochrome P450
IPR002401	Cytochrome P450, E-class, group I
IPR017972	Cytochrome P450, conserved site

Entry Information

Gene Name

carotene epsilon-monooxygenase

Protein Entry

LUT1_ARATH

UniProt ID

Q6TBX7

Species

Arabidopsis

Comments

Comment Type	Description
Catalytic Activity	Alpha-carotene + O(2) + AH(2) = alpha- cryptoxanthin + A + H(2)O.
Cofactor	Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
Disruption Phenotype	No visible phenotype, but lacks lutein and accumulates high levels of zeinoxanthin and beta, beta- xanthophylls. Triple mutant CYP97C1-BCH1-BCH2 is paler and smaller than wild-type. {ECO:0000269\|PubMed:12782726, ECO:0000269\|PubMed:14709673, ECO:0000269\|PubMed:16890225, ECO:0000269\|PubMed:18466360}.
Function	Heme-containing cytochrome P450 involved in the biosynthesis of xanthophylls. Specific for epsilon- and beta-ring hydroxylation of alpha-carotene. Has only a low activity toward the beta-rings of beta-carotene. The preferred substrate in planta is not alpha-carotene but the epsilon-ring of zeinoxanthin. {ECO:0000269\|PubMed:12782726, ECO:0000269\|PubMed:16890225, ECO:0000269\|PubMed:19147649, ECO:0000269\|PubMed:19939422}.
Sequence Caution	Sequence=AAM13903.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=CAB64216.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity	Belongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular Location	Plastid, chloroplast membrane {ECO:0000269\|PubMed:20061580}.

Unique RefSeq proteins for LMP010013 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
42565881	RefSeq	NP_190881	539	carotene epsilon-monooxygenase

Identical Sequences to LMP010013 proteins

Reference	Database	Accession	Length	Protein Name
GI:42565881	GenBank	ACW93595.1	539	Sequence 12508 from patent US 7569389
GI:42565881	GenBank	ACW94662.1	539	Sequence 13952 from patent US 7569389
GI:42565881	GenBank	AEE79040.1	539	carotene epsilon-monooxygenase [Arabidopsis thaliana]
GI:42565881	GenBank	AFX48940.1	539	Sequence 50773 from patent US 8299318
GI:42565881	GenBank	AGF14745.1	539	Sequence 14164 from patent US 8362325
GI:42565881	SwissProt	Q6TBX7.1	539	RecName: Full=Carotene epsilon-monooxygenase, chloroplastic; AltName: Full=Cytochrome P450 97C1; AltName: Full=Protein LUTEIN DEFICIENT 1; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010013 proteins

Reference	Database	Accession	Length	Protein Name
GI:42565881	EMBL	CAB64216.1	566	Cytochrom P450-like protein [Arabidopsis thaliana]
GI:42565881	GenBank	AAM13903.1	552	putative cytochrome P450, partial [Arabidopsis thaliana]
GI:42565881	GenBank	ACW93588.1	566	Sequence 12499 from patent US 7569389
GI:42565881	GenBank	ACW94661.1	552	Sequence 13951 from patent US 7569389
GI:42565881	RefSeq	XP_010504071.1	545	PREDICTED: carotene epsilon-monooxygenase, chloroplastic-like [Camelina sativa]
GI:42565881	RefSeq	XP_010426945.1	544	PREDICTED: carotene epsilon-monooxygenase, chloroplastic [Camelina sativa]