Gene/Proteome Database (LMPD)
LMPD ID
LMP009953
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
holocarboxylase synthase 1
Gene Symbol
Synonyms
F3N11.16; F3N11_16; HCS1; HOLOCARBOXYLASE SYNTHASE; holocarboxylase synthase 1
Alternate Names
holocarboxylase synthase 1
Chromosome
2
EC Number
6.3.4.-
Summary
Encodes holocarboxylase synthase.
Orthologs
Proteins
| holocarboxylase synthase 1 | |
|---|---|
| Refseq ID | NP_565605 |
| Protein GI | 18400914 |
| UniProt ID | Q9SL92 |
| mRNA ID | NM_128130 |
| Length | 367 |
| RefSeq Status | REVIEWED |
| MEAVRSTTTLSNFHLLNILVLRSLKPLHRLSFSFSASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVKDDDNSFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKNVWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQNVVTIQGLTSSGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI | |
Gene Information
Entrez Gene ID
Gene Name
holocarboxylase synthase 1
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0009507 | IDA:TAIR | C | chloroplast |
| GO:0005829 | IDA:TAIR | C | cytosol |
| GO:0009536 | IDA:TAIR | C | plastid |
| GO:0005524 | IEA:UniProtKB-KW | F | ATP binding |
| GO:0004077 | IDA:TAIR | F | biotin-[acetyl-CoA-carboxylase] ligase activity |
| GO:0004078 | IDA:TAIR | F | biotin-[methylcrotonoyl-CoA-carboxylase] ligase activity |
| GO:0042966 | IDA:TAIR | P | biotin carboxyl carrier protein biosynthetic process |
| GO:0006464 | IEA:InterPro | P | cellular protein modification process |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| PWY0-1264 | biotin-carboxyl carrier protein assembly |
REACTOME Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative initiation; Named isoforms=2; Name=HCS-ATG1; IsoId=Q9SL92-1; Sequence=Displayed; Name=HCS-ATG2; IsoId=Q9SL92-2; Sequence=VSP_053916; |
| Catalytic Activity | ATP + biotin + apo-[3-methylcrotonoyl- CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [3- methylcrotonoyl-CoA:carbon-dioxide ligase (ADP-forming)]. |
| Catalytic Activity | ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon- dioxide ligase (ADP-forming)]. |
| Disruption Phenotype | Embryonic lethality when homozygous due to aborted ovules that had not been fertilized. {ECO:0000269|PubMed:18156294}. |
| Function | Plays a major role in biotin-dependent carboxylase biotinylation. Catalyzes the addition of biotin to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase and can also biotinylate methylcrotonyl-CoA carboxylase. Is responsible for most, if not all, biotin--protein ligase activity in Arabidopsis. Is essential for plant viability and required for ovule development. {ECO:0000269|PubMed:18156294, ECO:0000269|PubMed:9173880, ECO:0000269|PubMed:9874227}. |
| Miscellaneous | The alternative splicing of the 5'UTR of HCS1 mRNA controls the dual targeting of HCS1 protein through alternative use of distinct initiation codons. A small ORF (uORF24) located in the HCS1 mRNA 5'UTR is essential for the AUG choice. The presence of uORF24 favors the synthesis of a short protein form initiated at the second AUG, which consequently localizes in the cytosol. In the absence of uORF24, the translation initiation begins at the first AUG, allowing the production of a HCS1 protein headed by a transit peptide (PubMed:18156294). {ECO:0000305|PubMed:18156294}. |
| Similarity | Belongs to the biotin--protein ligase family. {ECO:0000305}. |
| Similarity | Contains 1 BPL/LPL catalytic domain. {ECO:0000255|PROSITE-ProRule:PRU01067}. |
| Subcellular Location | Isoform HCS-ATG1: Plastid, chloroplast. |
| Subcellular Location | Isoform HCS-ATG2: Cytoplasm, cytosol. |
| Tissue Specificity | Expressed in roots, leaves, stems, flowers, siliques and seeds. {ECO:0000269|PubMed:11784724, ECO:0000269|PubMed:18156294}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP009953 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 18400914 | RefSeq | NP_565605 | 367 | holocarboxylase synthase 1 |
Identical Sequences to LMP009953 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18400914 | GenBank | ABD59086.1 | 367 | At2g25710 [Arabidopsis thaliana] |
| GI:18400914 | GenBank | AEC07740.1 | 367 | holocarboxylase synthase 1 [Arabidopsis thaliana] |
| GI:18400914 | GenBank | AEC07741.1 | 367 | holocarboxylase synthase 1 [Arabidopsis thaliana] |
| GI:18400914 | gnl | TIGR | 367 | biotin holocarboxylase synthetase [Arabidopsis thaliana] |
| GI:18400914 | RefSeq | NP_850067.1 | 367 | holocarboxylase synthase 1 [Arabidopsis thaliana] |
| GI:18400914 | SwissProt | Q9SL92.2 | 367 | RecName: Full=Biotin--protein ligase 1, chloroplastic; AltName: Full=Holocarboxylase synthetase 1; Includes: RecName: Full=Biotin--[methylcrotonoyl-CoA-carboxylase] ligase; Includes: RecName: Full=Biotin--[acetyl-CoA-carboxylase] ligase; Flags: Precursor [Arabidopsis thaliana] |
Related Sequences to LMP009953 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:18400914 | GenBank | AAL93108.1 | 367 | holocarboxylase synthetase 1 [Arabidopsis thaliana] |
| GI:18400914 | GenBank | EFH55136.1 | 365 | holocarboxylase synthetase 1 [Arabidopsis lyrata subsp. lyrata] |
| GI:18400914 | GenBank | EOA29337.1 | 357 | hypothetical protein CARUB_v10025619mg [Capsella rubella] |
| GI:18400914 | RefSeq | XP_002878877.1 | 365 | holocarboxylase synthetase 1 [Arabidopsis lyrata subsp. lyrata] |
| GI:18400914 | RefSeq | XP_006296439.1 | 357 | hypothetical protein CARUB_v10025619mg [Capsella rubella] |
| GI:18400914 | RefSeq | XP_010417356.1 | 369 | PREDICTED: biotin--protein ligase 2 isoform X4 [Camelina sativa] |