Gene/Proteome Database (LMPD)

LMPD ID
LMP009903
Gene ID
835334
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
beta-carotene hydroxylase 2
Gene Symbol
BETA-OHASE 2
Synonyms
B2; BCH2; BETA CAROTENOID HYDROXYLASE 2; beta-carotene hydroxylase 2; BETA-OHASE 2; CHY2; F6N7.5; F6N7_5; FERREDOXIN-DEPENDENT DIIRON OXYGENASE
Alternate Names
beta-carotene hydroxylase 2
Chromosome
5
EC Number
1.14.13.129

Proteins

beta-carotene hydroxylase 2
Refseq ID NP_001119420
Protein GI 186531513
UniProt ID Q9LTG0
mRNA ID NM_001125948
Length 234
RefSeq Status REVIEWED
MAAGLSTIAVTLKPLNRSSFSANHPISTAVFPPSLRFNGFRRRKILTVCFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRRIKLYNKGSSTS
beta-carotene hydroxylase 2
Refseq ID NP_200070
Protein GI 15237204
UniProt ID Q9LTG0
mRNA ID NM_124636
Length 303
RefSeq Status REVIEWED
MAAGLSTIAVTLKPLNRSSFSANHPISTAVFPPSLRFNGFRRRKILTVCFVVEERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRRIKLYNKGSSTS

Gene Information

Entrez Gene ID
835334
Gene Name
beta-carotene hydroxylase 2
Gene Symbol
BETA-OHASE 2
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009507 IEA:UniProtKB-KW C chloroplast
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0010291 IGI:TAIR F carotene beta-ring hydroxylase activity
GO:0016787 IEA:UniProtKB-KW F hydrolase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:0016119 IGI:TAIR P carotene metabolic process
GO:0006633 IEA:InterPro P fatty acid biosynthetic process
GO:0016123 IGI:TAIR P xanthophyll biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath_M00372 Abscisic acid biosynthesis, beta-carotene => abscisic acid
ath01110 Biosynthesis of secondary metabolites
ath00906 Carotenoid biosynthesis
ath01100 Metabolic pathways

Domain Information

InterPro Annotations

Accession Description
IPR006694 Fatty_acid_hydroxylase

UniProt Annotations

Entry Information

Gene Name
beta-carotene hydroxylase 2
Protein Entry
BCH2_ARATH
UniProt ID
Q9LTG0
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9LTG0-1; Sequence=Displayed; Name=2; IsoId=Q9LTG0-2; Sequence=VSP_041795; Note=Derived from EST data. No experimental confirmation available.;
Catalytic Activity Beta-carotene + 2 NADH + 2 O(2) = zeaxanthin + 2 NAD(+) + 2 H(2)O.
Disruption Phenotype No visible phenotype when grown under normal light conditions; due to the redundancy with BCH1. Bch1 and bch2 double mutant has no visible phenotype, but lower levels of beta, beta-xanthophylls and increased beta-carotene and lutein. Cyp97c1, bch1 and bch2 triple mutant is paler and smaller than wild-type. {ECO:0000269|PubMed:12782726, ECO:0000269|PubMed:16890225}.
Domain The histidine box domains may contain the active site and/or be involved in iron binding.
Function Nonheme diiron monooxygenase involved in the biosynthesis of xanthophylls. Specific for beta-ring hydroxylations of beta-carotene. Has also a low activity toward the beta- and epsilon-rings of alpha-carotene. No activity with acyclic carotenoids such as lycopene and neurosporene. Uses ferredoxin as an electron donor (Probable). {ECO:0000305|PubMed:11587509, ECO:0000305|PubMed:12782726, ECO:0000305|PubMed:16890225, ECO:0000305|PubMed:19147649}.
Similarity Belongs to the sterol desaturase family. {ECO:0000305}.
Subcellular Location Plastid, chloroplast membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
Subunit Homodimer. {ECO:0000250}.
Tissue Specificity Expressed in leaves, flowers, stems, roots and siliques. {ECO:0000269|PubMed:11587509}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009903 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15237204 RefSeq NP_200070 303 beta-carotene hydroxylase 2
186531513 RefSeq NP_001119420 234 beta-carotene hydroxylase 2

Identical Sequences to LMP009903 proteins

Reference Database Accession Length Protein Name
GI:15237204 DBBJ BAA98075.1 303 beta-carotene hydroxylase [Arabidopsis thaliana]
GI:15237204 GenBank AAL67090.1 303 putative beta-carotene hydroxylase [Arabidopsis thaliana]
GI:15237204 GenBank AAM51300.1 303 putative beta-carotene hydroxylase [Arabidopsis thaliana]
GI:15237204 gnl TAIR 303 beta-carotene hydroxylase 2 [Arabidopsis thaliana]
GI:186531513 gnl TAIR 234 beta-carotene hydroxylase 2 [Arabidopsis thaliana]
GI:15237204 SwissProt Q9LTG0.1 303 RecName: Full=Beta-carotene 3-hydroxylase 2, chloroplastic; Short=AtB2; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP009903 proteins

Reference Database Accession Length Protein Name
GI:186531513 DBBJ BAA98075.1 303 beta-carotene hydroxylase [Arabidopsis thaliana]
GI:186531513 GenBank AAL67090.1 303 putative beta-carotene hydroxylase [Arabidopsis thaliana]
GI:186531513 GenBank AAM51300.1 303 putative beta-carotene hydroxylase [Arabidopsis thaliana]
GI:15237204 GenBank AAP63869.1 305 Sequence 14 from patent US 6551807
GI:15237204 GenBank EFH42180.1 297 hypothetical protein ARALYDRAFT_495328 [Arabidopsis lyrata subsp. lyrata]
GI:15237204 GenBank EOA13755.1 304 hypothetical protein CARUB_v10026847mg [Capsella rubella]
GI:186531513 gnl TAIR 303 beta-carotene hydroxylase 2 [Arabidopsis thaliana]
GI:186531513 RefSeq NP_200070.1 303 beta-carotene hydroxylase 2 [Arabidopsis thaliana]
GI:15237204 RefSeq XP_002865921.1 297 hypothetical protein ARALYDRAFT_495328 [Arabidopsis lyrata subsp. lyrata]
GI:15237204 RefSeq XP_006280857.1 304 hypothetical protein CARUB_v10026847mg [Capsella rubella]
GI:15237204 RefSeq XP_010442795.1 302 PREDICTED: beta-carotene 3-hydroxylase 2, chloroplastic [Camelina sativa]
GI:186531513 SwissProt Q9LTG0.1 303 RecName: Full=Beta-carotene 3-hydroxylase 2, chloroplastic; Short=AtB2; Flags: Precursor [Arabidopsis thaliana]