Gene/Proteome Database (LMPD)

LMPD ID
LMP009784
Gene ID
837140
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
acyl-coenzyme A oxidase 3
Gene Symbol
ACX3
Synonyms
acyl-CoA oxidase 3; ATACX3
Alternate Names
acyl-coenzyme A oxidase 3
Chromosome
1
EC Number
1.3.3.6
Summary
Encodes an acyl-CoA oxidase with specificity for medium chain fatty acids.
Orthologs
View orthologs and multiple alignments for ACX3

Proteins

acyl-coenzyme A oxidase 3
Refseq ID NP_172119
Protein GI 15220946
UniProt ID P0CZ23
mRNA ID NM_100511
Length 675
RefSeq Status REVIEWED
MSDNRALRRAHVLANHILQSNPPSSNPSLSRELCLQYSPPELNESYGFDVKEMRKLLDGHNVVDRDWIYGLMMQSNLFNRKERGGKIFVSPDYNQTMEQQREITMKRIWYLLENGVFKGWLTETGPEAELRKLALLEVCGIYDHSVSIKVGVHFFLWGNAVKFFGTKRHHEKWLKNTEDYVVKGCFAMTELGHGSNVRGIETVTTYDPKTEEFVINTPCESAQKYWIGGAANHATHTIVFSQLHINGTNQGVHAFIAQIRDQDGSICPNIRIADCGHKIGLNGVDNGRIWFDNLRIPRENLLNAVADVSSDGKYVSSIKDPDQRFGAFMAPLTSGRVTIASSAIYSAKVGLSIAIRYSLSRRAFSVTANGPEVLLLDYPSHQRRLLPLLAKTYAMSFAANELKMIYVKRTPETNKAIHVVSSGFKAVLTWHNMHTLQECREAVGGQGVKTENLVGQLKGEFDVQTTFEGDNNVLMQQVSKALFAEYVSCKKRNKPFKGLGLEHMNSPRPVLPTQLTSSTLRCSQFQTNVFCLRERDLLEQFTSEVAQLQGRGESREFSFLLSHQLAEDLGKAFTEKAILQTILDAEAKLPTGSVKDVLGLVRSMYALISLEEDPSLLRYGYLSQDNVGDVRREVSKLCGELRPHALALVTSFGIPDSFLSPIAFNWVEANAWSSV

Gene Information

Entrez Gene ID
837140
Gene Name
acyl-coenzyme A oxidase 3
Gene Symbol
ACX3
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005777 IEA:UniProtKB-KW C peroxisome
GO:0003995 IEA:InterPro F acyl-CoA dehydrogenase activity
GO:0003997 TAS:TAIR F acyl-CoA oxidase activity
GO:0050660 IEA:InterPro F flavin adenine dinucleotide binding
GO:0006635 TAS:TAIR P fatty acid beta-oxidation
GO:0033540 IEA:UniProtKB-UniPathway P fatty acid beta-oxidation using acyl-CoA oxidase
GO:0051791 TAS:TAIR P medium-chain fatty acid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01040 Biosynthesis of unsaturated fatty acids
ath_M00113 Jasmonic acid biosynthesis
ath04146 Peroxisome
ath_M00087 beta-Oxidation

Domain Information

InterPro Annotations

Accession Description
IPR009075 Acyl-CoA dehydrogenase/oxidase C-terminal
IPR009100 Acyl-CoA dehydrogenase/oxidase, N-terminal and middle domain
IPR012258 Acyl-CoA oxidase
IPR002655 Acyl-CoA oxidase, C-terminal
IPR006091 Acyl-CoA oxidase/dehydrogenase, central domain

UniProt Annotations

Entry Information

Gene Name
acyl-coenzyme A oxidase 3
Protein Entry
ACOX3_ARATH
UniProt ID
P0CZ23
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=3.7 uM for lauroyl-CoA; pH dependence: Optimum pH is 8.5-9.0.;
Catalytic Activity Acyl-CoA + O(2) = trans-2,3-dehydroacyl-CoA + H(2)O(2).
Cofactor Name=FAD; Xref=ChEBI:CHEBI:57692;
Developmental Stage Induced by seed imbibition with a peak at day 2 and then declines steadily until day 7. Not detected in developing seeds. Constitutive expression in root axis. {ECO:0000269|PubMed:10859203}.
Function Catalyzes the desaturation of medium-chain acyl-CoAs to 2-trans-enoyl-CoAs. Active on C8:0- to C14:0-CoA with a maximal activity on C12:0-CoA.
Induction Not induced by dehydration or abscisic acid (ABA). {ECO:0000269|PubMed:15141068}.
Pathway Lipid metabolism; peroxisomal fatty acid beta-oxidation.
Similarity Belongs to the acyl-CoA oxidase family. {ECO:0000305}.
Subcellular Location Peroxisome {ECO:0000269|PubMed:10918060}.
Tissue Specificity Most abundant in flowers and senescing rosette leaves. Lower expression in hypocotyls, stems, young rosette leaves, cotyledons, cauline leaves and root tip of young seedlings. {ECO:0000269|PubMed:10859203, ECO:0000269|PubMed:10918060}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009784 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15220946 RefSeq NP_172119 675 acyl-coenzyme A oxidase 3

Identical Sequences to LMP009784 proteins

Reference Database Accession Length Protein Name
GI:15220946 EMBL CBU96018.1 675 unnamed protein product [Arabidopsis thaliana]
GI:15220946 GenBank AEE27972.1 675 acyl-coenzyme A oxidase 3 [Arabidopsis thaliana]
GI:15220946 GenBank AGX52695.1 675 Sequence 5745 from patent US 8541208
GI:15220946 GenBank AGX57632.1 675 Sequence 15607 from patent US 8541208
GI:15220946 GenBank AGX63416.1 675 Sequence 26936 from patent US 8541208
GI:15220946 SwissProt P0CZ23.1 675 RecName: Full=Acyl-coenzyme A oxidase 3, peroxisomal; Short=AOX 3; Short=Acyl-CoA oxidase 3; AltName: Full=Medium-chain acyl-CoA oxidase; Short=AtCX3; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP009784 proteins

Reference Database Accession Length Protein Name
GI:15220946 GenBank AAF73843.1 675 acyl-CoA oxidase ACX3 [Arabidopsis thaliana]
GI:15220946 GenBank AAO00645.1 675 Sequence 8 from patent US 6489461
GI:15220946 GenBank EFH65856.1 675 acyl-CoA oxidase ACX3 [Arabidopsis lyrata subsp. lyrata]
GI:15220946 GenBank EOA39249.1 675 hypothetical protein CARUB_v10012245mg [Capsella rubella]
GI:15220946 RefSeq XP_002889597.1 675 acyl-CoA oxidase ACX3 [Arabidopsis lyrata subsp. lyrata]
GI:15220946 RefSeq XP_006306351.1 675 hypothetical protein CARUB_v10012245mg [Capsella rubella]