Gene/Proteome Database (LMPD)

LMPD ID
LMP009722
Gene ID
828523
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3-oxo-Delta(4,5)-steroid 5-beta-reductase
Gene Symbol
VEP1
Synonyms
AWI31; T22A6.50; T22A6_50; VEIN PATTERNING 1; VEP1
Alternate Names
3-oxo-Delta(4,5)-steroid 5-beta-reductase
Chromosome
4
EC Number
1.3.1.3
Summary
encodes a novel protein containing mammalian death domain involved in programmed cell death. Gene is expressed in vascular system and mutants carrying dominant mutation in the gene have defective vascular patterning. Gene expression is induced specifically by wounding.
Orthologs
View orthologs and multiple alignments for VEP1

Proteins

3-oxo-Delta(4,5)-steroid 5-beta-reductase
Refseq ID NP_194153
Protein GI 15233747
UniProt ID Q9STX2
mRNA ID NM_118555
Length 388
RefSeq Status REVIEWED
MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQIQNFYYTQEDILFEEIKKIETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP
3-oxo-Delta(4,5)-steroid 5-beta-reductase
Refseq ID NP_001078438
Protein GI 145333900
UniProt ID Q9STX2
mRNA ID NM_001084969
Length 387
RefSeq Status REVIEWED
MSWWWAGAIGAAKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYGVARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANGSMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNVDGPRHDPPFTEDMPRLQIQNFYYTQEDILFEEIKKIETVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLLFPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFGIEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDSMNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP

Gene Information

Entrez Gene ID
828523
Gene Name
3-oxo-Delta(4,5)-steroid 5-beta-reductase
Gene Symbol
VEP1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:TAIR C cytosol
GO:0047787 IEA:UniProtKB-EC F delta4-3-oxosteroid 5beta-reductase activity
GO:0035671 IDA:TAIR F enone reductase activity
GO:0009611 IEP:TAIR P response to wounding
GO:0008202 IDA:TAIR P steroid metabolic process
GO:0010051 IMP:TAIR P xylem and phloem pattern formation

Domain Information

InterPro Annotations

Accession Description
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
3-oxo-Delta(4,5)-steroid 5-beta-reductase
Protein Entry
VEP1_ARATH
UniProt ID
Q9STX2
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9STX2-1; Sequence=Displayed; Name=2; IsoId=Q9STX2-2; Sequence=VSP_044368; Note=Derived from EST data. No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=268 uM for progesterone {ECO:0000269|PubMed:19166903, ECO:0000269|PubMed:22357344}; KM=755 uM for cortisol {ECO:0000269|PubMed:19166903, ECO:0000269|PubMed:22357344}; KM=1423 uM for 4-androstene-3,17-dione {ECO:0000269|PubMed:19166903, ECO:0000269|PubMed:22357344}; KM=764 uM for cortexone {ECO:0000269|PubMed:19166903, ECO:0000269|PubMed:22357344}; KM=5 uM for NADPH {ECO:0000269|PubMed:19166903, ECO:0000269|PubMed:22357344}; KM=116 uM for 2-cyclohexen-1-one {ECO:0000269|PubMed:19166903, ECO:0000269|PubMed:22357344}; Note=kcat is 10.11 min(-1) with progesterone as substrate. kcat is 66.85 min(-1) with 2-cyclohexen-1-one as substrate.; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:19166903, ECO:0000269|PubMed:22357344}; Temperature dependence: Optimum temperature is 45 degrees Celsius. {ECO:0000269|PubMed:19166903, ECO:0000269|PubMed:22357344};
Catalytic Activity 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP(+) = cortisone.
Catalytic Activity 5-beta-cholestan-3-one + NADP(+) = cholest-4- en-3-one + NADPH.
Function Involved in vascular strand development. Catalyzes the stereospecific conversion of progesterone to 5-beta-pregnane-3,20- dione. Can use progesterone, testosterone, 21-acetyl cortexone, 2- cyclohexenone, but-1-en-3-one, ethyl acrylate, ethylmethacrylate, cortisone and canarigenone as substrates, lower activity with 3- methyl-2-cyclohexenone and 3,5,5-trimethyl-2-cyclohexenone as substrate, and no activity with canarigenin, canarigenin digitoxoside and pregnenolone. May be involved in the formation of 5-beta phytoecdysteroids. {ECO:0000269|PubMed:11917087, ECO:0000269|PubMed:19166903}.
Induction Up-regulated by wounding. Not induced by drought, high salt, low temperature or herbicide treatment. {ECO:0000269|PubMed:11917087, ECO:0000269|PubMed:9085278}.
Sequence Caution Sequence=CAA68126.1; Type=Frameshift; Positions=225, 273, 301; Evidence={ECO:0000305};
Similarity Belongs to the short-chain dehydrogenases/reductases (SDR) family. Highly divergent. {ECO:0000305}.
Subunit Homodimer. {ECO:0000269|PubMed:19166903}.
Tissue Specificity Expressed in roots, stems, leaves, flowers, seeds and siliques. Expressed in the vascular bundles. {ECO:0000269|PubMed:11917087, ECO:0000269|PubMed:19166903}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009722 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15233747 RefSeq NP_194153 388 3-oxo-Delta(4,5)-steroid 5-beta-reductase
145333900 RefSeq NP_001078438 387 3-oxo-Delta(4,5)-steroid 5-beta-reductase

Identical Sequences to LMP009722 proteins

Reference Database Accession Length Protein Name
GI:15233747 GenBank ABU55811.1 388 progesterone 5-beta-reductase [Arabidopsis thaliana]
GI:15233747 GenBank ACW93427.1 388 Sequence 12282 from patent US 7569389
GI:15233747 GenBank ACW93824.1 388 Sequence 12817 from patent US 7569389
GI:15233747 GenBank ACW94441.1 388 Sequence 13653 from patent US 7569389
GI:15233747 GenBank AEE84868.1 388 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Arabidopsis thaliana]
GI:145333900 GenBank AEE84869.1 387 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Arabidopsis thaliana]
GI:15233747 SwissProt Q9STX2.1 388 RecName: Full=3-oxo-Delta(4,5)-steroid 5-beta-reductase; AltName: Full=Delta(4)-3-oxosteroid 5-beta-reductase; AltName: Full=Delta-4,5-steroid 5-beta-reductase; Short=At5beta-StR; AltName: Full=Progesterone 5-beta-reductase; Short=5beta-POR; AltName: Full=Protein VEIN PATTERNING 1 [Arabidopsis thaliana]

Related Sequences to LMP009722 proteins

Reference Database Accession Length Protein Name
GI:145333900 EMBL CAB45057.1 388 putative protein [Arabidopsis thaliana]
GI:145333900 GenBank AAP37838.1 388 At4g24220 [Arabidopsis thaliana]
GI:145333900 GenBank ACW93427.1 388 Sequence 12282 from patent US 7569389
GI:145333900 GenBank ACW93824.1 388 Sequence 12817 from patent US 7569389
GI:145333900 GenBank ACW94441.1 388 Sequence 13653 from patent US 7569389
GI:15233747 GenBank ADG56544.1 389 progesterone 5-beta-reductase [Erysimum crepidifolium]
GI:15233747 GenBank AEE84869.1 387 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Arabidopsis thaliana]
GI:15233747 GenBank EOA16797.1 388 hypothetical protein CARUB_v10005016mg [Capsella rubella]
GI:15233747 RefSeq NP_001078438.1 387 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Arabidopsis thaliana]
GI:15233747 RefSeq XP_006283899.1 388 hypothetical protein CARUB_v10005016mg [Capsella rubella]
GI:15233747 RefSeq XP_010448593.1 388 PREDICTED: 3-oxo-Delta(4,5)-steroid 5-beta-reductase [Camelina sativa]
GI:145333900 SwissProt Q9STX2.1 388 RecName: Full=3-oxo-Delta(4,5)-steroid 5-beta-reductase; AltName: Full=Delta(4)-3-oxosteroid 5-beta-reductase; AltName: Full=Delta-4,5-steroid 5-beta-reductase; Short=At5beta-StR; AltName: Full=Progesterone 5-beta-reductase; Short=5beta-POR; AltName: Full=Protein VEIN PATTERNING 1 [Arabidopsis thaliana]