Gene/Proteome Database (LMPD)
Proteins
| 3-oxoacyl-[acyl-carrier-protein] synthase | |
|---|---|
| Refseq ID | NP_178533 |
| Protein GI | 22325473 |
| UniProt ID | Q8L3X9 |
| mRNA ID | NM_126485 |
| Length | 461 |
| RefSeq Status | REVIEWED |
| MATSNLRRHLSASRLRLNRFISTSSSYHSHRRVVVTGLGMVTPLGRGVETTWRRLIDGECGIRGLTLDDLKMKSFDEETKLYTFDQLSSKVAAFVPYGSNPGEFDEALWLNSKAVANFIGYAVCAADEALRDAEWLPTEEEEKERTGVSIGGGIGSICDIVEAAQLICEKRLRRLSPFFIPKILVNMASGHVSMKYGFQGPNHAAVTACATGAHSIGDATRMIQFGDADVMVAGGTESSIDALSVAGFSRSRALSTKFNSSPQEASRPFDCDRDGFVIGEGSGVIVLEEYEHAKRRGAKIYAELCGYGMSGDAHHITQPPEDGKGAVLAMTRALRQSGLCPNQIDYVNAHATSTPIGDAVEARAIKTVFSEHATSGTLAFSSTKGATGHLLGAAGAVEAIFSILAIHHGVAPMTLNVKNPDPIFDKRFMPLTTSKKMLVRTAMSNSFGFGGTNASLLFASI | |
Gene Information
Entrez Gene ID
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase
Gene Symbol
Species
Arabidopsis thaliana
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005739 | IEA:UniProtKB-KW | C | mitochondrion |
| GO:0004315 | IEA:UniProtKB-EC | F | 3-oxoacyl-[acyl-carrier-protein] synthase activity |
| GO:0006633 | IEA:UniProtKB-UniPathway | P | fatty acid biosynthetic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ath00780 | Biotin metabolism |
| ath00061 | Fatty acid biosynthesis |
| ath_M00083 | Fatty acid biosynthesis, elongation |
| ath01212 | Fatty acid metabolism |
Domain Information
UniProt Annotations
Entry Information
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase
Protein Entry
KASM_ARATH
UniProt ID
Species
Arabidopsis
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Acyl-[acyl-carrier-protein] + malonyl-[acyl- carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]. |
| Disruption Phenotype | Slow growth and bleached leaf phenotype when grown under ambient air, but normal growth under CO(2)-enriched air. Highly prevents lipoylation of the H-protein subunit of the glycine decarboxylase (GDC) in leaves, but has only a limited effect on the lipoylation of the E2 subunits of pyruvate dehydrogenase (PDH) and alpha-ketoacid dehydrogenase (KGDH) complexes in leaves and even no effect in roots. {ECO:0000269|PubMed:17616510}. |
| Enzyme Regulation | Inhibited by cerulenin. |
| Function | Catalyzes all the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Able to elongate saturated acyl chains from 4 to at least 16 carbons. Uses malonyl-CoA but not acetyl-CoA as primer substrate. When expressed in a heterologous system, reveals a bimodal distribution of products, with peaks at C8 and C14-C16. The major product of the reaction (octanoyl-ACP) is required for the lipoylation of essential mitochondrial proteins. {ECO:0000269|PubMed:17616510}. |
| Miscellaneous | Mitochondrial protein lipoylation in leaves does not exclusively depend on the lipoate biosynthesis by KAS and may occur independently of this pathway in roots. {ECO:0000305|PubMed:17616510}. |
| Pathway | Lipid metabolism; fatty acid biosynthesis. |
| Sequence Caution | Sequence=AAD25826.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
| Similarity | Belongs to the beta-ketoacyl-ACP synthases family. {ECO:0000305}. |
| Subcellular Location | Mitochondrion {ECO:0000269|PubMed:14660674}. |
| Subunit | Homodimer. {ECO:0000269|PubMed:15527780}. |
| Tissue Specificity | Expressed at the same level in leaves, roots and flowers. {ECO:0000269|PubMed:14660674}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP009721 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 22325473 | RefSeq | NP_178533 | 461 | 3-oxoacyl-[acyl-carrier-protein] synthase |
Identical Sequences to LMP009721 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:22325473 | EMBL | CBN70928.1 | 461 | unnamed protein product [Arabidopsis thaliana] |
| GI:22325473 | EMBL | CBN69320.1 | 461 | unnamed protein product [Arabidopsis thaliana] |
| GI:22325473 | GenBank | AAM20439.1 | 461 | 3-oxoacyl carrier protein synthase [Arabidopsis thaliana] |
| GI:22325473 | GenBank | AAM91232.1 | 461 | 3-oxoacyl carrier protein synthase [Arabidopsis thaliana] |
| GI:22325473 | GenBank | AEC05844.1 | 461 | 3-oxoacyl-[acyl-carrier-protein] synthase [Arabidopsis thaliana] |
| GI:22325473 | SwissProt | Q8L3X9.1 | 461 | RecName: Full=3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial; AltName: Full=Beta-ketoacyl-ACP synthase; AltName: Full=mtKAS; Flags: Precursor [Arabidopsis thaliana] |
Related Sequences to LMP009721 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:22325473 | GenBank | EFH62019.1 | 460 | hypothetical protein ARALYDRAFT_480093 [Arabidopsis lyrata subsp. lyrata] |
| GI:22325473 | gnl | TIGR | 442 | 3-oxoacyl carrier protein synthase [Arabidopsis thaliana] |
| GI:22325473 | PDB | 1W0I | 431 | Chain B, Arabidopsis Thaliana Mitochondrial Kas |
| GI:22325473 | RefSeq | XP_002885760.1 | 460 | hypothetical protein ARALYDRAFT_480093 [Arabidopsis lyrata subsp. lyrata] |
| GI:22325473 | RefSeq | XP_010514616.1 | 473 | PREDICTED: 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial-like isoform X1 [Camelina sativa] |
| GI:22325473 | RefSeq | XP_010467007.1 | 465 | PREDICTED: 3-oxoacyl-[acyl-carrier-protein] synthase, mitochondrial [Camelina sativa] |