Gene/Proteome Database (LMPD)

LMPD ID
LMP009691
Gene ID
839395
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3-ketoacyl-CoA synthase 1
Gene Symbol
KCS1
Synonyms
3-ketoacyl-CoA synthase 1; KCS1; T25K16.11; T25K16_11
Alternate Names
3-ketoacyl-CoA synthase 1
Chromosome
1
EC Number
2.3.1.119
Summary
Encodes a condensing enzyme KCS1 (3-ketoacyl-CoA synthase 1) which is involved in the critical fatty acid elongation process in wax biosynthesis.
Orthologs
View orthologs and multiple alignments for KCS1

Proteins

3-ketoacyl-CoA synthase 1
Refseq ID NP_171620
Protein GI 22329274
UniProt ID Q9MAM3
mRNA ID NM_099994
Length 528
RefSeq Status REVIEWED
MERTNSIEMDRERLTAEMAFRDSSSAVIRIRRRLPDLLTSVKLKYVKLGLHNSCNVTTILFFLIILPLTGTVLVQLTGLTFDTFSELWSNQAVQLDTATRLTCLVFLSFVLTLYVANRSKPVYLVDFSCYKPEDERKISVDSFLTMTEENGSFTDDTVQFQQRISNRAGLGDETYLPRGITSTPPKLNMSEARAEAEAVMFGALDSLFEKTGIKPAEVGILIVNCSLFNPTPSLSAMIVNHYKMREDIKSYNLGGMGCSAGLISIDLANNLLKANPNSYAVVVSTENITLNWYFGNDRSMLLCNCIFRMGGAAILLSNRRQDRKKSKYSLVNVVRTHKGSDDKNYNCVYQKEDERGTIGVSLARELMSVAGDALKTNITTLGPMVLPLSEQLMFLISLVKRKMFKLKVKPYIPDFKLAFEHFCIHAGGRAVLDEVQKNLDLKDWHMEPSRMTLHRFGNTSSSSLWYEMAYTEAKGRVKAGDRLWQIAFGSGFKCNSAVWKALRPVSTEEMTGNAWAGSIDQYPVKVVQ

Gene Information

Entrez Gene ID
839395
Gene Name
3-ketoacyl-CoA synthase 1
Gene Symbol
KCS1
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0022626 IDA:TAIR C cytosolic ribosome
GO:0005783 IDA:TAIR C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0016020 ISS:TAIR C membrane
GO:0009922 IDA:TAIR F fatty acid elongase activity
GO:0033807 IEA:UniProtKB-EC F icosanoyl-CoA synthase activity
GO:0006633 IEA:UniProtKB-UniPathway P fatty acid biosynthetic process
GO:0009409 IEP:TAIR P response to cold
GO:0009416 IEP:TAIR P response to light stimulus
GO:0010025 IDA:TAIR P wax biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
ath01110 Biosynthesis of secondary metabolites
ath_M00415 Fatty acid biosynthesis, elongation, endoplasmic reticulum
ath00062 Fatty acid elongation

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6803 phosphatidylcholine acyl editing

Domain Information

InterPro Annotations

Accession Description
IPR013747 3-Oxoacyl-[acyl-carrier-protein (ACP)] synthase III C-terminal
IPR013601 FAE1/Type III polyketide synthase-like protein
IPR016039 Thiolase-like
IPR016038 Thiolase-like, subgroup
IPR012392 Very-long-chain 3-ketoacyl-CoA synthase

UniProt Annotations

Entry Information

Gene Name
3-ketoacyl-CoA synthase 1
Protein Entry
KCS1_ARATH
UniProt ID
Q9MAM3
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity A very-long-chain acyl-CoA + malonyl-CoA = CoA + a very-long-chain 3-oxoacyl-CoA + CO(2).
Catalytic Activity Stearoyl-CoA + malonyl-CoA + 2 NAD(P)H = icosanoyl-CoA + CO(2) + CoA + 2 NAD(P)(+) + H(2)O.
Caution It is uncertain whether Met-1 or Met-9 is the initiator. {ECO:0000305}.
Disruption Phenotype Plants have thinner stems with an altered wax composition, and are more sensitive to dehydration. {ECO:0000269|PubMed:10074711}.
Enzyme Regulation Inhibited by K3 herbicides such as alachlor, allidochlor, anilofos, cafenstrole, fentrazamide and flufenacet. {ECO:0000269|PubMed:15277688}.
Function Contributes to cuticular wax and suberin biosynthesis. Involved in both decarbonylation and acyl-reduction wax synthesis pathways. Elongase condensing enzyme mostly active with saturated fatty acids, especially with 16:0, 16:1, 18:0, and 20:0. Mediates the synthesis of VLCFAs from 20 to 26 carbons in length (e.g. C20:1, C20, C22, C24 and C26). {ECO:0000269|PubMed:10074711, ECO:0000269|PubMed:15277688, ECO:0000269|PubMed:16765910}.
Induction Repressed by herbicides such as flufenacet and benfuresate. {ECO:0000269|PubMed:12916765}.
Pathway Lipid metabolism; fatty acid biosynthesis.
Sequence Caution Sequence=BAD95286.1; Type=Erroneous initiation; Evidence={ECO:0000305};
Similarity Belongs to the chalcone/stilbene synthases family. {ECO:0000305}.
Similarity Contains 1 FAE (fatty acid elongase) domain. {ECO:0000305}.
Subcellular Location Membrane {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
Tissue Specificity Ubiquitous. {ECO:0000269|PubMed:10074711}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009691 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
22329274 RefSeq NP_171620 528 3-ketoacyl-CoA synthase 1

Identical Sequences to LMP009691 proteins

Reference Database Accession Length Protein Name
GI:22329274 GenBank AAF26470.1 528 T25K16.11 [Arabidopsis thaliana]
GI:22329274 GenBank AAL66982.1 528 putative fatty acid elongase 3-ketoacyl-CoA synthase 1 [Arabidopsis thaliana]
GI:22329274 GenBank AAM20218.1 528 putative fatty acid elongase 3-ketoacyl-CoA synthase 1 [Arabidopsis thaliana]
GI:22329274 GenBank AEE27241.1 528 3-ketoacyl-CoA synthase 1 [Arabidopsis thaliana]
GI:22329274 SwissProt Q9MAM3.1 528 RecName: Full=3-ketoacyl-CoA synthase 1; Short=KCS-1; AltName: Full=Very long-chain fatty acid condensing enzyme 1; Short=VLCFA condensing enzyme 1 [Arabidopsis thaliana]

Related Sequences to LMP009691 proteins

Reference Database Accession Length Protein Name
GI:22329274 DBBJ BAE98925.1 528 putative fatty acid elongase 3-ketoacyl-CoA synthase 1 [Arabidopsis thaliana]
GI:22329274 EMBL CAW45909.1 528 unnamed protein product [Zea mays]
GI:22329274 EMBL CAW71169.1 528 unnamed protein product [Zea mays]
GI:22329274 EMBL CBC11411.1 528 unnamed protein product [Zea mays]
GI:22329274 EMBL CBC60843.1 528 unnamed protein product [Zea mays]
GI:22329274 GenBank EFH65642.1 528 3-ketoacyl-CoA synthase 1 [Arabidopsis lyrata subsp. lyrata]