Gene/Proteome Database (LMPD)

LMPD ID
LMP009684
Gene ID
841733
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
3-deoxy-manno-octulosonate cytidylyltransferase
Gene Symbol
KDSB
Alternate Names
3-deoxy-manno-octulosonate cytidylyltransferase
Chromosome
1
EC Number
2.7.7.38
Summary
Encodes a putative CMP-KDO (3-deoxy-D-manno-octulosonate) synthetase.
Orthologs
View orthologs and multiple alignments for KDSB

Proteins

3-deoxy-manno-octulosonate cytidylyltransferase
Refseq ID NP_175708
Protein GI 30695393
UniProt ID Q9C920
mRNA ID NM_104178
Length 290
RefSeq Status REVIEWED
MSVCSSSSSSQKTWIVNGILAGTAIAAAIGARAYLGRSKKFRSRVVGIIPARYASSRFEGKPLVQILGKPMIQRTWERSKLATTLDHIVVATDDERIAECCRGFGADVIMTSESCRNGTERCNEALEKLEKKYDVVVNIQGDEPLIEPEIIDGVVKALQVTPDAVFSTAVTSLKPEDGLDPNRVKCVVDNRGYAIYFSRGLIPYNKSGKVNPDFPYMLHLGIQSFDSKFLKVYSELQPTPLQQEEDLEQLKVLENGYKMKVIKVDHEAHGVDTPDDVEKIESLMRERNMS

Gene Information

Entrez Gene ID
841733
Gene Name
3-deoxy-manno-octulosonate cytidylyltransferase
Gene Symbol
KDSB
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0031307 IDA:TAIR C integral component of mitochondrial outer membrane
GO:0005739 IDA:TAIR C mitochondrion
GO:0008690 IDA:TAIR F 3-deoxy-manno-octulosonate cytidylyltransferase activity
GO:0033468 IDA:TAIR P CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process
GO:0071555 IEA:UniProtKB-KW P cell wall organization
GO:0009103 IEA:InterPro P lipopolysaccharide biosynthetic process
GO:0009555 IMP:TAIR P pollen development
GO:0009860 IMP:TAIR P pollen tube growth

KEGG Pathway Links

KEGG Pathway ID Description
ath01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-1269 CMP-KDO biosynthesis I
PWY-5111 CMP-KDO biosynthesis II (from D-arabinose 5-phosphate)
KDO-NAGLIPASYN-PWY superpathway of (KDO)2-lipid A biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR004528 3-deoxy-D-manno-octulosonate cytidylyltransferase
IPR003329 Acylneuraminate cytidylyltransferase
IPR029044 Nucleotide-diphospho-sugar transferases

UniProt Annotations

Entry Information

Gene Name
3-deoxy-manno-octulosonate cytidylyltransferase
Protein Entry
KDSB_ARATH
UniProt ID
Q9C920
Species
Arabidopsis

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is 9.5. {ECO:0000269|PubMed:21893514};
Catalytic Activity CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate. {ECO:0000269|PubMed:19914588}.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Function Catalyzes the production of the sugar nucleotide CMP-3- deoxy-D-manno-octulosonate (CMP-KDO). CTP is the preferred nucleotide donor, but it can partially be replaced with UTP. Activates KDO during the biosynthesis of rhamnogalacturonan II (RG-II), a structurally complex pectic polysaccharide of the primary cell wall. RG-II is essential for the cell wall integrity of rapidly growing tissues and pollen tube growth and elongation. {ECO:0000269|PubMed:19914588, ECO:0000269|PubMed:21893514}.
Miscellaneous Rhamnogalacturonan II (RG-II) RG-II is a structurally complex pectic polysaccharide present in the primary cell wall. RG-II consists of a linear 1,4-linked a-Dgalacturonic acid backbone with four distinct side chains, two of which contain apiosyl residues. Boric acid forms a diester with two apiosyl residues from separate RG-II molecules, thereby covalently cross- linking two RG-II molecules as a dimer.
Pathway Nucleotide-sugar biosynthesis; CMP-3-deoxy-D-manno- octulosonate biosynthesis; CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP: step 1/1.
Similarity Belongs to the KdsB family. {ECO:0000305}.
Subcellular Location Mitochondrion outer membrane {ECO:0000305|PubMed:21893514, ECO:0000305|PubMed:21896887}; Peripheral membrane protein {ECO:0000305|PubMed:21893514, ECO:0000305|PubMed:21896887}.
Tissue Specificity Expressed in roots, leaves, stems and siliques. {ECO:0000269|PubMed:19914588}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009684 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30695393 RefSeq NP_175708 290 3-deoxy-manno-octulosonate cytidylyltransferase

Identical Sequences to LMP009684 proteins

Reference Database Accession Length Protein Name
GI:30695393 DBBJ BAH20217.1 290 AT1G53000 [Arabidopsis thaliana]
GI:30695393 EMBL CAD43603.1 290 putative CMP-KDO synthetase [Arabidopsis thaliana]
GI:30695393 GenBank AAG52266.1 290 putative deoxyoctulonosic acid synthetase; 116195-114393 [Arabidopsis thaliana]
GI:30695393 GenBank AEE32875.1 290 3-deoxy-manno-octulosonate cytidylyltransferase [Arabidopsis thaliana]
GI:30695393 SwissProt Q9C920.1 290 RecName: Full=3-deoxy-manno-octulosonate cytidylyltransferase, mitochondrial; AltName: Full=CMP-2-keto-3-deoxyoctulosonic acid synthase; Short=AtCKS; Short=CMP-KDO synthase; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP009684 proteins

Reference Database Accession Length Protein Name
GI:30695393 GenBank EFH70676.1 293 cytidylyltransferase family [Arabidopsis lyrata subsp. lyrata]
GI:30695393 GenBank EOA38369.1 292 hypothetical protein CARUB_v10009902mg [Capsella rubella]
GI:30695393 RefSeq XP_002894417.1 293 cytidylyltransferase family [Arabidopsis lyrata subsp. lyrata]
GI:30695393 RefSeq XP_006305471.1 292 hypothetical protein CARUB_v10009902mg [Capsella rubella]
GI:30695393 RefSeq XP_006392844.1 291 hypothetical protein EUTSA_v10011698mg [Eutrema salsugineum]
GI:30695393 RefSeq XP_010479831.1 292 PREDICTED: 3-deoxy-manno-octulosonate cytidylyltransferase, mitochondrial [Camelina sativa]