Gene/Proteome Database (LMPD)

LMPD ID
LMP009666
Gene ID
820647
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
phosphatidylinositol-3P 5-kinase-like
Gene Symbol
FAB1B
Synonyms
FORMS APLOID AND BINUCLEATE CELLS 1B
Alternate Names
phosphatidylinositol-3P 5-kinase-like
Chromosome
3
EC Number
2.7.1.150
Summary
Encodes a protein that is predicted to act as a 1-phosphatidylinositol-3-phosphate (PtdIns3P) 5-kinase based on its homology to Fab1 from yeast. It contains an FYVE domain required for binding to PtdIns3P-containing membranes in yeast, as well as a Cpn60_TCP1 homology domain plus a kinase domain. fab1a/fab1b pollen grains not viable and have defective vacuolar organization.
Orthologs
View orthologs and multiple alignments for FAB1B

Proteins

phosphatidylinositol-3P 5-kinase-like
Refseq ID NP_188044
Protein GI 15231820
UniProt ID Q9LUM0
mRNA ID NM_112285
Length 1791
RefSeq Status REVIEWED
MGTRDSNNRTFSEIVGLIKSWLPWRSEPATVSRDFWMPDQSCRVCYECDCQFTLINRRHHCRHCGRVFCGKCTANSIPFAPSDLRTPREDWERIRVCNYCFRQWEQGDGGPHVSNITELSTSPSETSLLSSKTSTTANSSSFALGSMPGLIGLNQRVHHGSDVSLHGVSSMETSVTKQGKETSRRSSFIATDVEDPSRFALNSIRSDDEYDEYGAYQTDIETSHSPRANEYYGPMEYNGMGIDDVPCKHLGGETADQKSLSGSPLIHQCLESLIREGSEQFQKKSEHDGRDECEASSPADISDDQVVEPVDFENNGLLWVPPEPENEEDERESALFDEEDNEGDASGEWGYLRPSTSFGSGEYRGEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLLKPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGGGLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDISLVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGKKVVKTLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGASPELPLNSPITVALPDKSTSIERSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELLSTTTNLSIQKDIPPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSGRSVPVDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNEKPKETQSQKEEFPPSPSDHQSILVSLSSRSVWKGTVCERSHLFRIKYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQDYLLPGEKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVACCGHSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFNYENQDWIQRETDEVIERAELLFSEVLNAISQIAEKGFRRRIGELEEVLQKEKAEFEENMQKILHREVNEGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDTKREENEKPPLAKSQTLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLYQEKDDGGEVSPSKTLPDTSYPLENKVDVRRTQSDGQIVMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTMSNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLNFYRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPTSMIAYALMSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFDPSRSNGSVDESILSISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICLPSELEYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLSESISTKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRARYNPDSSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVGVDEEKNELVLGIIDFLRQYTWDKHLESWVKFTGILGGPKNEAPTVISPKQYKRRFRKAMTTYFLMVPDQWSPPNVVANNSKSDQPEETSQAGTQAE

Gene Information

Entrez Gene ID
820647
Gene Name
phosphatidylinositol-3P 5-kinase-like
Gene Symbol
FAB1B
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005768 IDA:TAIR C endosome
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0005739 IDA:TAIR C mitochondrion
GO:0000285 IGI:TAIR F 1-phosphatidylinositol-3-phosphate 5-kinase activity
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0046872 IEA:UniProtKB-KW F metal ion binding
GO:0044267 IEA:InterPro P cellular protein metabolic process
GO:0010256 IGI:TAIR P endomembrane system organization
GO:0046854 IGI:GOC P phosphatidylinositol phosphorylation
GO:0009555 IGI:TAIR P pollen development
GO:0090332 IMP:TAIR P stomatal closure
GO:0007033 IGI:TAIR P vacuole organization

KEGG Pathway Links

KEGG Pathway ID Description
ath00562 Inositol phosphate metabolism
ath04145 Phagosome

REACTOME Pathway Links

REACTOME Pathway ID Description
6254338 Synthesis of PIPs at the early endosome membrane
6254335 Synthesis of PIPs at the late endosome membrane

Domain Information

InterPro Annotations

Accession Description
IPR002423 Chaperonin Cpn60/TCP-1
IPR000306 FYVE zinc finger
IPR027409 GroEL-like apical domain
IPR027483 Phosphatidylinositol-4-phosphate 5-kinase, C-terminal
IPR027484 Phosphatidylinositol-4-phosphate 5-kinase, N-terminal domain
IPR002498 Phosphatidylinositol-4-phosphate 5-kinase, core
IPR017455 Zinc finger, FYVE-related
IPR011011 Zinc finger, FYVE/PHD-type
IPR013083 Zinc finger, RING/FYVE/PHD-type

UniProt Annotations

Entry Information

Gene Name
phosphatidylinositol-3P 5-kinase-like
Protein Entry
FAB1B_ARATH
UniProt ID
Q9LUM0
Species
Arabidopsis

Comments

Comment Type Description
Catalytic Activity ATP + 1-phosphatidyl-1D-myo-inositol 3- phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
Disruption Phenotype Leaf-curling phenotype. Root growth inhibition, root gravitropic response, and hyposensitivity to exogenous auxin. Fab1a and fab1b double mutant displays an abnormal vacuolar phenotype late in pollen development leading to inviable pollen (PubMed:19846542). {ECO:0000269|PubMed:19846542, ECO:0000269|PubMed:21173023, ECO:0000269|PubMed:21412048}.
Function The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo- inositol ring, to form phosphatidylinositol 3,5-bisphosphate (By similarity). Plays an important role in maintenance of endomembrane homeostasis including endocytosis, vacuole formation, and vacuolar acidification processes. Required for development of viable pollen. Might mediate recycling of auxin transporters. {ECO:0000250, ECO:0000269|PubMed:19846542, ECO:0000269|PubMed:21173023, ECO:0000269|PubMed:21412048}.
Similarity Contains 1 FYVE-type zinc finger. {ECO:0000255|PROSITE-ProRule:PRU00091}.
Similarity Contains 1 PIPK domain. {ECO:0000255|PROSITE- ProRule:PRU00781}.
Subcellular Location Endosome membrane {ECO:0000269|PubMed:21173023}; Peripheral membrane protein {ECO:0000269|PubMed:21173023}.
Subunit Component of the PI(3,5)P2 regulatory complex at least composed of ATG18, SAC/FIG4, FAB1 and VAC14. {ECO:0000250}.
Tissue Specificity Ubiquitous with highest expression levels in the root hair zone, pollen, and stamens. {ECO:0000269|PubMed:19846542}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009666 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
15231820 RefSeq NP_188044 1791 phosphatidylinositol-3P 5-kinase-like

Identical Sequences to LMP009666 proteins

Reference Database Accession Length Protein Name
GI:15231820 DBBJ BAB01033.1 1791 unnamed protein product [Arabidopsis thaliana]
GI:15231820 GenBank AEE75496.1 1791 phosphatidylinositol-3P 5-kinase-like [Arabidopsis thaliana]
GI:15231820 SwissProt Q9LUM0.1 1791 RecName: Full=1-phosphatidylinositol-3-phosphate 5-kinase FAB1B; Short=Phosphatidylinositol 3-phosphate 5-kinase; AltName: Full=FYVE finger-containing phosphoinositide kinase; AltName: Full=PIKfyve; AltName: Full=Phosphatidylinositol 3-phosphate 5-kinase type III; Short=PIPkin-III; Short=Type III PIP kinase; AltName: Full=Protein FORMS APLOID AND BINUCLEATE CELLS 1B [Arabidopsis thaliana]

Related Sequences to LMP009666 proteins

Reference Database Accession Length Protein Name
GI:15231820 GenBank EFH59133.1 1789 phosphatidylinositol-4-phosphate 5-kinase family protein [Arabidopsis lyrata subsp. lyrata]
GI:15231820 GenBank ESQ48550.1 1788 hypothetical protein EUTSA_v10019882mg [Eutrema salsugineum]
GI:15231820 RefSeq XP_002882874.1 1789 phosphatidylinositol-4-phosphate 5-kinase family protein [Arabidopsis lyrata subsp. lyrata]
GI:15231820 RefSeq XP_006407097.1 1788 hypothetical protein EUTSA_v10019882mg [Eutrema salsugineum]
GI:15231820 RefSeq XP_010465319.1 1781 PREDICTED: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B-like [Camelina sativa]
GI:15231820 RefSeq XP_010487226.1 1781 PREDICTED: 1-phosphatidylinositol-3-phosphate 5-kinase FAB1B [Camelina sativa]