Gene/Proteome Database (LMPD)

LMPD ID
LMP009184
Gene ID
44544
Species
Drosophila melanogaster (Drosophila)
Gene Name
Hepatocyte nuclear factor 4
Gene Symbol
Hnf4
Synonyms
CG9310; dHNF-4; dHNF4; Dmel\CG9310; DmHNF4; Hnf-4; HNF-4; HNF-4(D); Hnf-4h; HNF4; HNF4alpha; NR2A4
Chromosome
2L
Map Location
29E4-29E4

Proteins

hepatocyte nuclear factor 4, isoform A
Refseq ID NP_476887
Protein GI 24582907
UniProt ID P49866
mRNA ID NM_057539
Length 704
RefSeq Status REVIEWED
MMKHPQDLSVTDDQQLMKVNKVEKMEQELHDPESESHIMHADALASAYPAASQPHSPIGLALSPNGGGLGLSNSSNQSSENFALCNGNGNAGSAGGGSASSGSNNNNSMFSPNNNLSGSGSGTNSSQQQLNQQQSPTVCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHQYTCRFARNCVVDKDKRNQCRYCRLRKCFKAGMKKEAVQNERDRISCRRTSNDDPDPGNGLSVISLVKAENESRQSKAGAAMEPNINEDLSNKQFASINDVCESMKQQLLTLVEWAKQIPAFNELQLDDQVALLRAHAGEHLLLGLSRRSMHLKDVLLLSNNCVITRHCPDPLVSPNLDISRIGARIIDELVTVMKDVGIDDTEFACIKALVFFDPNAKGLNEPHRIKSLRHQILNNLEDYISDRQYESRGRFGEILLILPVLQSITWQMIEQIQFAKIFGVAHIDSLLQEMLLGGELADNPLPLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGPGSHSLDLEVQHIQALIEANSADDSFRAYAASTAAAAAAAVSSSSSAPASVAPASISPPLNSPKSQHQHQQHATHNESSYLDMPVKHYNGSRSGPLPTQHSPQRMHPYQRAVASPVEVSSGGGGLGLRNPADITLNEYNRSEGSSAEELLRRTPLKIRAPEMLTAPAGYGTEPCRMTLKQEPETGY
hepatocyte nuclear factor 4, isoform B
Refseq ID NP_723413
Protein GI 24582909
UniProt ID P49866
mRNA ID NM_164833
Length 732
RefSeq Status REVIEWED
MVRKSGRVKISSRDRVAVGNILLRGKVGGGRVAVAAAEEAEAGRRRRRRDSSASRTASSDESESHIMHADALASAYPAASQPHSPIGLALSPNGGGLGLSNSSNQSSENFALCNGNGNAGSAGGGSASSGSNNNNSMFSPNNNLSGSGSGTNSSQQQLNQQQSPTVCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHQYTCRFARNCVVDKDKRNQCRYCRLRKCFKAGMKKEAVQNERDRISCRRTSNDDPDPGNGLSVISLVKAENESRQSKAGAAMEPNINEDLSNKQFASINDVCESMKQQLLTLVEWAKQIPAFNELQLDDQVALLRAHAGEHLLLGLSRRSMHLKDVLLLSNNCVITRHCPDPLVSPNLDISRIGARIIDELVTVMKDVGIDDTEFACIKALVFFDPNAKGLNEPHRIKSLRHQILNNLEDYISDRQYESRGRFGEILLILPVLQSITWQMIEQIQFAKIFGVAHIDSLLQEMLLGGELADNPLPLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGPGSHSLDLEVQHIQALIEANSADDSFRAYAASTAAAAAAAVSSSSSAPASVAPASISPPLNSPKSQHQHQQHATHNESSYLDMPVKHYNGSRSGPLPTQHSPQRMHPYQRAVASPVEVSSGGGGLGLRNPADITLNEYNRSEGSSAEELLRRTPLKIRAPEMLTAPAGYGTEPCRMTLKQEPETGY
hepatocyte nuclear factor 4, isoform D
Refseq ID NP_001097126
Protein GI 161076819
UniProt ID P49866
mRNA ID NM_001103656
Length 708
RefSeq Status REVIEWED
MYASMLPSLLNMKTENLTSSSYDDAFLLEENLLHIMESESHIMHADALASAYPAASQPHSPIGLALSPNGGGLGLSNSSNQSSENFALCNGNGNAGSAGGGSASSGSNNNNSMFSPNNNLSGSGSGTNSSQQQLNQQQSPTVCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHQYTCRFARNCVVDKDKRNQCRYCRLRKCFKAGMKKEAVQNERDRISCRRTSNDDPDPGNGLSVISLVKAENESRQSKAGAAMEPNINEDLSNKQFASINDVCESMKQQLLTLVEWAKQIPAFNELQLDDQVALLRAHAGEHLLLGLSRRSMHLKDVLLLSNNCVITRHCPDPLVSPNLDISRIGARIIDELVTVMKDVGIDDTEFACIKALVFFDPNAKGLNEPHRIKSLRHQILNNLEDYISDRQYESRGRFGEILLILPVLQSITWQMIEQIQFAKIFGVAHIDSLLQEMLLGGELADNPLPLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGPGSHSLDLEVQHIQALIEANSADDSFRAYAASTAAAAAAAVSSSSSAPASVAPASISPPLNSPKSQHQHQQHATHNESSYLDMPVKHYNGSRSGPLPTQHSPQRMHPYQRAVASPVEVSSGGGGLGLRNPADITLNEYNRSEGSSAEELLRRTPLKIRAPEMLTAPAGYGTEPCRMTLKQEPETGY
hepatocyte nuclear factor 4, isoform E
Refseq ID NP_723414
Protein GI 442626918
UniProt ID P49866
mRNA ID NM_164834
Length 704
RefSeq Status REVIEWED
Protein sequence is identical to GI:24582907 (mRNA isoform)
hepatocyte nuclear factor 4, isoform F
Refseq ID NP_001285758
Protein GI 665407203
UniProt ID X2J9X1
mRNA ID NM_001298829
Length 666
RefSeq Status REVIEWED
MHADALASAYPAASQPHSPIGLALSPNGGGLGLSNSSNQSSENFALCNGNGNAGSAGGGSASSGSNNNNSMFSPNNNLSGSGSGTNSSQQQLNQQQSPTVCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHQYTCRFARNCVVDKDKRNQCRYCRLRKCFKAGMKKEAVQNERDRISCRRTSNDDPDPGNGLSVISLVKAENESRQSKAGAAMEPNINEDLSNKQFASINDVCESMKQQLLTLVEWAKQIPAFNELQLDDQVALLRAHAGEHLLLGLSRRSMHLKDVLLLSNNCVITRHCPDPLVSPNLDISRIGARIIDELVTVMKDVGIDDTEFACIKALVFFDPNAKGLNEPHRIKSLRHQILNNLEDYISDRQYESRGRFGEILLILPVLQSITWQMIEQIQFAKIFGVAHIDSLLQEMLLGGELADNPLPLSPPNQSNDYQSPTHTGNMEGGNQVNSSLDSLATSGGPGSHSLDLEVQHIQALIEANSADDSFRAYAASTAAAAAAAVSSSSSAPASVAPASISPPLNSPKSQHQHQQHATHNESSYLDMPVKHYNGSRSGPLPTQHSPQRMHPYQRAVASPVEVSSGGGGLGLRNPADITLNEYNRSEGSSAEELLRRTPLKIRAPEMLTAPAGYGTEPCRMTLKQEPETGY

Gene Information

Entrez Gene ID
44544
Gene Name
Hepatocyte nuclear factor 4
Gene Symbol
Hnf4
Species
Drosophila melanogaster

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005634 IEA:UniProtKB-KW C nucleus
GO:0004879 NAS:FlyBase F ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity
GO:0043565 IEA:InterPro F sequence-specific DNA binding
GO:0003707 IEA:InterPro F steroid hormone receptor activity
GO:0008270 IEA:InterPro F zinc ion binding
GO:0030522 NAS:GOC P intracellular receptor signaling pathway
GO:0016042 IDA:FlyBase P lipid catabolic process
GO:0034440 IDA:FlyBase P lipid oxidation

REACTOME Pathway Links

REACTOME Pathway ID Description
6226115 Developmental Biology
6225926 Gene Expression
6226331 Generic Transcription Pathway
6226616 Nuclear Receptor transcription pathway
6226557 Regulation of beta-cell development
6226561 Regulation of gene expression in beta cells

Domain Information

InterPro Annotations

Accession Description
IPR008946 Nuclear hormone receptor, ligand-binding
IPR000536 Nuclear hormone receptor, ligand-binding, core
IPR000003 Retinoid X receptor/HNF4
IPR001723 Steroid hormone receptor
IPR013088 Zinc finger, NHR/GATA-type
IPR001628 Zinc finger, nuclear hormone receptor-type

UniProt Annotations

Entry Information

Gene Name
Hepatocyte nuclear factor 4
Protein Entry
HNF4_DROME
UniProt ID
X2J9X1
Species
Drosophila

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=3; Name=A; IsoId=P49866-1; Sequence=Displayed; Name=D; IsoId=P49866-2; Sequence=VSP_054577; Note=No experimental confirmation available.; Name=B; IsoId=P49866-3; Sequence=VSP_054578;
Disruption Phenotype Under normal culture conditions, the flies progress through development until they die during or shortly after adult eclosion. Under low density culture conditions, many flies survive and develop into morphologically normal adults. The mutant flies are, however, very sensitive to starvation, displaying inability to efficiently mobilize stored lipid in the midgut and fat body, increased levels of long-chain fatty acids and triglycerides, and premature death. {ECO:0000269|PubMed:19254568}.
Function Transcriptionally controlled transcription factor. Important for the differentiation of various specialized cell types that arise from both endoderm and mesoderm. May have a role in early gut formation. Plays an essential role in lipid catabolism, regulating lipid mobilization and beta-oxidation in response to nutrient deprivation. {ECO:0000269|PubMed:19254568, ECO:0000269|PubMed:8440243}.
Sequence Caution Sequence=AAB09592.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAN71555.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=ACS68165.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};
Similarity Belongs to the nuclear hormone receptor family. NR2 subfamily. {ECO:0000305}.
Similarity Contains 1 nuclear receptor DNA-binding domain. {ECO:0000255|PROSITE-ProRule:PRU00407}.
Subcellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00407, ECO:0000269|PubMed:19254568}.
Subunit Homodimer. {ECO:0000269|PubMed:8440243}.
Tissue Specificity In third instar larvae, expressed at high levels in midgut and attached gastic caeca, fat body, Malpighian tubules and oenocytes, and at lower levels in proventriculus, salivary glands, epidermis, brain and ring gland. Not detected in imaginal disks and the median neurosecretory cells that produce insulin-like peptides (at protein level). In developing embryos, expressed in mid-gut, fat bodies and the distal region of Malpighian tubules. {ECO:0000269|PubMed:19254568, ECO:0000269|PubMed:8440243}.

Identical and Related Proteins

Unique RefSeq proteins for LMP009184 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
24582907 RefSeq NP_476887 704 hepatocyte nuclear factor 4, isoform A
24582909 RefSeq NP_723413 732 hepatocyte nuclear factor 4, isoform B
161076819 RefSeq NP_001097126 708 hepatocyte nuclear factor 4, isoform D
665407203 RefSeq NP_001285758 666 hepatocyte nuclear factor 4, isoform F

Identical Sequences to LMP009184 proteins

Reference Database Accession Length Protein Name
GI:24582907 GenBank AAO39537.1 704 RE09535p [Drosophila melanogaster]
GI:24582907 GenBank ACL83805.1 704 Hnf4-PA, partial [synthetic construct]
GI:24582907 GenBank ACL88913.1 704 Hnf4-PA [synthetic construct]
GI:24582909 gnl FlyBase 732 hepatocyte nuclear factor 4, isoform B [Drosophila melanogaster]
GI:161076819 gnl FlyBase 708 hepatocyte nuclear factor 4, isoform D [Drosophila melanogaster]
GI:24582907 gnl FlyBase 704 hepatocyte nuclear factor 4, isoform E [Drosophila melanogaster]
GI:665407203 gnl FlyBase 666 hepatocyte nuclear factor 4, isoform F [Drosophila melanogaster]
GI:24582907 RefSeq NP_723414.2 704 hepatocyte nuclear factor 4, isoform E [Drosophila melanogaster]
GI:24582907 SwissProt P49866.3 704 RecName: Full=Transcription factor HNF-4 homolog; Short=dHNF4; AltName: Full=Nuclear receptor subfamily 2 group A member 4 [Drosophila melanogaster]

Related Sequences to LMP009184 proteins

Reference Database Accession Length Protein Name
GI:24582909 GenBank AAN71555.1 738 RH29074p, partial [Drosophila melanogaster]
GI:665407203 GenBank AAO39537.1 704 RE09535p [Drosophila melanogaster]
GI:161076819 GenBank AAO39537.1 704 RE09535p [Drosophila melanogaster]
GI:24582909 GenBank AAO39537.1 704 RE09535p [Drosophila melanogaster]
GI:24582907 GenBank EDV58288.1 704 GG24056 [Drosophila erecta]
GI:24582907 GenBank EDW52184.1 703 GM12759 [Drosophila sechellia]
GI:24582907 GenBank EDX04281.1 703 GD22387 [Drosophila simulans]
GI:161076819 GenBank ACL83805.1 704 Hnf4-PA, partial [synthetic construct]
GI:24582909 GenBank ACL83805.1 704 Hnf4-PA, partial [synthetic construct]
GI:665407203 GenBank ACL83805.1 704 Hnf4-PA, partial [synthetic construct]
GI:161076819 GenBank ACL88913.1 704 Hnf4-PA [synthetic construct]
GI:24582909 GenBank ACL88913.1 704 Hnf4-PA [synthetic construct]
GI:665407203 GenBank ACL88913.1 704 Hnf4-PA [synthetic construct]
GI:24582909 GenBank ACS68165.1 738 FI04795p, partial [Drosophila melanogaster]
GI:665407203 gnl FlyBase 704 hepatocyte nuclear factor 4, isoform E [Drosophila melanogaster]
GI:24582909 RefSeq NP_476887.2 704 hepatocyte nuclear factor 4, isoform A [Drosophila melanogaster]
GI:665407203 RefSeq NP_476887.2 704 hepatocyte nuclear factor 4, isoform A [Drosophila melanogaster]
GI:161076819 RefSeq NP_476887.2 704 hepatocyte nuclear factor 4, isoform A [Drosophila melanogaster]
GI:24582907 RefSeq XP_001969229.1 704 GG24056 [Drosophila erecta]
GI:24582907 RefSeq XP_002036261.1 703 GM12759 [Drosophila sechellia]
GI:24582907 RefSeq XP_002078696.1 703 GD22387 [Drosophila simulans]
GI:161076819 RefSeq NP_723414.2 704 hepatocyte nuclear factor 4, isoform E [Drosophila melanogaster]
GI:161076819 SwissProt P49866.3 704 RecName: Full=Transcription factor HNF-4 homolog; Short=dHNF4; AltName: Full=Nuclear receptor subfamily 2 group A member 4 [Drosophila melanogaster]
GI:665407203 SwissProt P49866.3 704 RecName: Full=Transcription factor HNF-4 homolog; Short=dHNF4; AltName: Full=Nuclear receptor subfamily 2 group A member 4 [Drosophila melanogaster]