Gene/Proteome Database (LMPD)
Proteins
| lipoic acid synthase | |
|---|---|
| Refseq ID | NP_524183 |
| Protein GI | 221513272 |
| UniProt ID | Q7JQW6 |
| mRNA ID | NM_079459 |
| Length | 377 |
| RefSeq Status | REVIEWED |
| MLRALKTHAEAPIVVATRAASTNAEKLEEIRERLAKGPNFHDFVQNPDNTRNEWEQYDGKLRREKGEEQRLRLPPWLKTTIPVGKNYAKIKAQMRELKLSTVCEEARCPNIGECWGGGEHGTQTATIMLMGDTCTRGCRFCSVKTARKPPPLDVNEPVNTATAIASWGLDYIVLTSVDRDDLPDGGSEHIAETVREIKARNSNIFVECLVPDFRGNLECVKTIANSGLDVYAHNIETVEKLTPYVRDRRAHYRQTLQVLTEAKRFNPNLITKSSIMLGLGETDEEIENTLKDLREAGVDCVTLGQYMQPTNKHLKVIEYVTPEKFKHWEERGNELGFLYTASGPLVRSSYKAGEFFITSILENRKKRQNDTEVPKKQ | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005739 | IEA:UniProtKB-HAMAP | C | mitochondrion |
| GO:0051539 | IEA:UniProtKB-HAMAP | F | 4 iron, 4 sulfur cluster binding |
| GO:0016992 | IEA:UniProtKB-HAMAP | F | lipoate synthase activity |
| GO:0046872 | IEA:UniProtKB-KW | F | metal ion binding |
| GO:0009249 | IEA:UniProtKB-HAMAP | P | protein lipoylation |
KEGG Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | Protein N(6)-(octanoyl)lysine + 2 sulfur- (sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)- (lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'- deoxyadenosine. |
| Cofactor | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000255|HAMAP-Rule:MF_03123}; Note=Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L- methionine. {ECO:0000255|HAMAP-Rule:MF_03123}; |
| Function | Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives. {ECO:0000255|HAMAP-Rule:MF_03123}. |
| Interaction | Q7K1H5:CG11808; NbExp=1; IntAct=EBI-126542, EBI-159475; |
| Pathway | Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 2/2. {ECO:0000255|HAMAP-Rule:MF_03123}. |
| Sequence Caution | Sequence=AAL13786.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=AAO41414.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; |
| Similarity | Belongs to the radical SAM superfamily. Lipoyl synthase family. {ECO:0000255|HAMAP-Rule:MF_03123}. |
| Subcellular Location | Mitochondrion {ECO:0000255|HAMAP- Rule:MF_03123}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP009028 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 221513272 | RefSeq | NP_524183 | 377 | lipoic acid synthase |
Identical Sequences to LMP009028 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:221513272 | gnl | FlyBase | 377 | lipoic acid synthase [Drosophila melanogaster] |
| GI:221513272 | SwissProt | Q7JQW6.2 | 377 | RecName: Full=Lipoyl synthase, mitochondrial; AltName: Full=Lipoate synthase; Short=LS; Short=Lip-syn; AltName: Full=Lipoic acid synthase; Flags: Precursor [Drosophila melanogaster] |
Related Sequences to LMP009028 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:221513272 | EMBL | CBM36051.1 | 380 | unnamed protein product [Drosophila melanogaster] |
| GI:221513272 | EMBL | CBN72408.1 | 380 | unnamed protein product [Drosophila melanogaster] |
| GI:221513272 | GenBank | AAL13786.1 | 380 | LD24887p [Drosophila melanogaster] |
| GI:221513272 | GenBank | AAO41414.1 | 380 | RH26374p [Drosophila melanogaster] |
| GI:221513272 | GenBank | EDW44220.1 | 377 | GM22215 [Drosophila sechellia] |
| GI:221513272 | GenBank | ACL89904.1 | 380 | Las-PC [synthetic construct] |