LIPID MAPS

LMPD ID

LMP008039

Gene ID

180919

Species

Caenorhabditis elegans (C. elegans)

Gene Name

Protein PEPT-1

Gene Symbol

pept-1

Synonyms

CELE_K04E7.2

Alternate Names

Protein PEPT-1

Chromosome

X

Protein PEPT-1
Refseq ID	NP_509087
Protein GI	17569141
UniProt ID	Q21219
mRNA ID	NM_076686
Length	835
RefSeq Status	REVIEWED
MGYSESRSESVSSKGKTSYGHELETVPLPEKKIYTTWPDMIRHWPKTTLCIVSNEFCERFSYYGMRTVLTFYLLNVLKFTDSQSTIFFNGFTVLCYTTPLLGSIVADGYIGKFWTIFSVSILYAIGQVVLALASTKNFQSSVHPWMDLSGLLIIAFGTGGIKPCVSAFGGDQFELGQERMLSLFFSMFYFSINAGSMISTFISPIFRSQPCLGQDSCYPMAFGIPAILMIVATLVFMGGSFWYKKNPPKDNVFGEVSRLMFRAVGNKMKSGSTPKEHWLLHYLTTHDCALDAKCLELQAEKRNKNLCQKKKFIDDVRSLLRVLVMFLPVPMFWALYDQQGSVWLIQAIQMDCRLSDTLLLLPDQMQTLNAVLILLFIPLFQVIIYPVAAKCVRLTPLRKMVTGGLLASLAFLITGFVQLQVNTTLPTLPEEGEASISFWNQFETDCTITVMSGIHKRVLPHDKYLHEDKKNKSGIYNLFTTKSPAKGNGDWTLTYDLSYDGACGDTSKLEKTVKVTAKSKKIIYVGVGSFGYYQNTANTDKPTDGTGEFSMGIVTVFNSSYGGNFAMCRQNTSDFDVNHPCNPRHPADFYFWETDYNSHTDDRDQNATITGSLSSQPAVTYKQKSVKPGYWQLYYLLNTPKDVDRQTYNKTATLVAPTNYGFHRVKQGGVFIYALTGTYENPKIHELQIVQSNSVSILWQIPQIVVITAAEILFSITGYEFAYSQSAPSMKALVQALWLLTTAAGDSIIVVITILNLFENMAVEFFVYAAAMFVVIAIFALLSIFYYTYNYYTTDEEDGEIGVDDEEEIEDHNPRYSIDNKGFHPDEKDTFDMHF

Entrez Gene ID

180919

Gene Name

Protein PEPT-1

Gene Symbol

pept-1

Species

Caenorhabditis elegans

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0016324	IDA:WormBase	C	apical plasma membrane
GO:0016021	IEA:UniProtKB-KW	C	integral component of membrane
GO:0042936	IMP:WormBase	F	dipeptide transporter activity
GO:0080144	IMP:WormBase	P	amino acid homeostasis
GO:0008340	IGI:WormBase	P	determination of adult lifespan
GO:0042938	IMP:WormBase	P	dipeptide transport
GO:0019915	IMP:WormBase	P	lipid storage
GO:2000192	IMP:WormBase	P	negative regulation of fatty acid transport
GO:0002119	IMP:WormBase	P	nematode larval development
GO:0040010	IMP:WormBase	P	positive regulation of growth rate
GO:0040018	IMP:WormBase	P	positive regulation of multicellular organism growth
GO:0015031	IEA:UniProtKB-KW	P	protein transport
GO:0032006	IMP:WormBase	P	regulation of TOR signaling
GO:1900101	IMP:WormBase	P	regulation of endoplasmic reticulum unfolded protein response
GO:0006885	IGI:WormBase	P	regulation of pH
GO:0000003	IMP:WormBase	P	reproduction
GO:0009408	IMP:WormBase	P	response to heat
GO:0006979	IMP:WormBase	P	response to oxidative stress

REACTOME Pathway Links

REACTOME Pathway ID	Description
5653215	Proton/oligonucleotide cotransporters
5652505	SLC-mediated transmembrane transport
5652506	Transmembrane transport of small molecules
5653121	Transport of inorganic cations/anions and amino acids/oligopeptides

InterPro Annotations

Accession	Description
IPR004768	Oligopeptide transporter
IPR018456	PTR2 family proton/oligopeptide symporter, conserved site
IPR000109	Proton-dependent oligopeptide transporter family

Entry Information

Gene Name

Protein PEPT-1

Protein Entry

PEPT1_CAEEL

UniProt ID

Q21219

Species

C. elegans

Comments

Comment Type	Description
Developmental Stage	Biphasic expression pattern observed. Gradual increase in expression during development from embryo through to adult with a decrease during larva stages 1 and 2. {ECO:0000269\|PubMed:9601088}.
Disruption Phenotype	Mutant worms have decreased body size, high fat stores, increased uptake of fatty acids, reduced brood size, retarded postembryonic growth, extended reproductive life span and increased resistance to stress. RNAi-mediated knockdown of the protein results in impaired synthesis of long-chain and polyunsaturated fatty acids. Pharyngeal pumping rate is similar to wild type but simultaneous knockdown with nhx-2 results in reduced rate of pharyngeal pumping and slightly higher acidic intestinal pH. {ECO:0000269\|PubMed:12939266, ECO:0000269\|PubMed:15155758, ECO:0000269\|PubMed:18676815, ECO:0000269\|PubMed:19621081, ECO:0000269\|PubMed:19844570, ECO:0000269\|PubMed:20550516}.
Function	Low-affinity peptide transporter that is necessary for proton-dependent uptake of di- or tripeptides, and to a minor extent tetrapeptides, in the intestine. Transport is independent of sodium and chloride ions. Controls the uptake of dietary fatty acids, plays a role in fatty acid synthesis and is responsible for dipeptide-induced acidification of the intestine. Regulates cellular pH differences together with the antiporter protein, nhx- 2. Amino acid uptake and absorption levels influence the insulin signaling/daf-2 and let-363/TOR pathways, subsequently affecting the stress response and longevity of the organism. {ECO:0000269\|PubMed:12939266, ECO:0000269\|PubMed:15155758, ECO:0000269\|PubMed:19621081, ECO:0000269\|PubMed:20550516, ECO:0000269\|PubMed:21275419, ECO:0000269\|PubMed:21980510, ECO:0000269\|PubMed:9601088}.
Sequence Caution	Sequence=AAC39119.1; Type=Frameshift; Positions=11; Evidence={ECO:0000305};
Similarity	Belongs to the PTR2/POT transporter (TC 2.A.17) family. {ECO:0000305}.
Subcellular Location	Apical cell membrane {ECO:0000269\|PubMed:12939266, ECO:0000269\|PubMed:21980510}; Multi- pass membrane protein {ECO:0000269\|PubMed:12939266, ECO:0000269\|PubMed:21980510}. Note=Colocalizes with nhx-2 along the apical membrane of the intestinal cells.
Tissue Specificity	Expressed specifically in the intestine. {ECO:0000269\|PubMed:12939266}.

Unique RefSeq proteins for LMP008039 (as displayed in Record Overview)

Protein GI	Database	Accession	Length	Protein Name
17569141	RefSeq	NP_509087	835	Protein PEPT-1

Identical Sequences to LMP008039 proteins

Reference	Database	Accession	Length	Protein Name
GI:17569141	EMBL	CCD64583.1	835	Protein PEPT-1 [Caenorhabditis elegans]
GI:17569141	SwissProt	Q21219.2	835	RecName: Full=Peptide transporter family 1; AltName: Full=Di-/tri-peptide transporter CPTB; AltName: Full=Oligopeptide transporter 1 [Caenorhabditis elegans]

Related Sequences to LMP008039 proteins

Reference	Database	Accession	Length	Protein Name
GI:17569141	EMBL	CAP25647.1	838	Protein CBR-PEPT-1 [Caenorhabditis briggsae]
GI:17569141	GenBank	AAC39119.1	796	low-affinity peptide transporter [Caenorhabditis elegans]
GI:17569141	GenBank	EFO82401.1	855	CRE-PEPT-1 protein [Caenorhabditis remanei]
GI:17569141	GenBank	EGT43101.1	840	hypothetical protein CAEBREN_30646 [Caenorhabditis brenneri]
GI:17569141	RefSeq	XP_002644887.1	838	C. briggsae CBR-OPT-2 protein [Caenorhabditis briggsae]
GI:17569141	RefSeq	XP_003117803.1	855	CRE-PEPT-1 protein [Caenorhabditis remanei]