Gene/Proteome Database (LMPD)

LMPD ID
LMP007752
Gene ID
948326
Species
Escherichia coli K-12 (E. coli)
Gene Name
3-octaprenyl-4-hydroxybenzoate decarboxylase
Gene Symbol
ubiD
Synonyms
ECK3835; JW3819; yigC; yigY
Summary
AFMB Structural Genomics target Number 3 (http://afmb.cnrs-mrs.fr/article171. [More information is available at EcoGene: EG11396]. 3-Octaprenyl-4-hydroxybenzoate decarboxylase catalyzes the third step in the biosynthesis of ubiquinone. [More information is available at EcoCyc: EG11396].
Orthologs
View orthologs and multiple alignments for ubiD

Proteins

3-octaprenyl-4-hydroxybenzoate decarboxylase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_418285
Protein GI 16131689
UniProt ID P0AAB4
Length 497
RefSeq Status REVIEWED
MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLRAGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPKGFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLITWGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVSVALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGYIEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGVALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQFMYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSKMGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA

Gene Information

Entrez Gene ID
948326
Gene Name
3-octaprenyl-4-hydroxybenzoate decarboxylase
Gene Symbol
ubiD
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005886 IEA:UniProtKB-KW C plasma membrane
GO:0008694 IDA:EcoCyc F 3-octaprenyl-4-hydroxybenzoate carboxy-lyase activity
GO:0010181 IEA:InterPro F FMN binding
GO:0042802 IDA:EcoCyc F identical protein binding
GO:0016491 IEA:InterPro F oxidoreductase activity
GO:0006744 IMP:EcoCyc P ubiquinone biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco01110 Biosynthesis of secondary metabolites
eco01100 Metabolic pathways
eco00130 Ubiquinone and other terpenoid-quinone biosynthesis
ko00130 Ubiquinone and other terpenoid-quinone biosynthesis
M00117 Ubiquinone biosynthesis, prokaryotes, chorismate => ubiquinone
eco_M00117 Ubiquinone biosynthesis, prokaryotes, chorismate => ubiquinone

BIOCYC Pathway Links

BIOCYC Pathway ID Description
ALL-CHORISMATE-PWY superpathway of chorismate metabolism
ALL-CHORISMATE-PWY superpathway of chorismate metabolism
UBISYN-PWY superpathway of ubiquinol-8 biosynthesis (prokaryotic)
UBISYN-PWY superpathway of ubiquinol-8 biosynthesis (prokaryotic)
PWY-6708 ubiquinol-8 biosynthesis (prokaryotic)
PWY-6708 ubiquinol-8 biosynthesis (prokaryotic)

Domain Information

InterPro Annotations

Accession Description
IPR012349 FMN-binding split barrel
IPR023677 OPHB_Carboxy-lyase
IPR002830 UbiD decarboxylyase family

UniProt Annotations

Entry Information

Gene Name
3-octaprenyl-4-hydroxybenzoate decarboxylase
Protein Entry
UBID_ECOLI
UniProt ID
P0AAB4
Species
E. coli

Comments

Comment Type Description
Cofactor Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:782527}; Note=Divalent metal ions. Preferably manganese. {ECO:0000269|PubMed:782527};
Enzyme Regulation Requires phospholipid, a metal ion, dithiothreitol and at least one other so far unidentified soluble cofactor for maximal activity. Inhibited by EDTA. {ECO:0000269|PubMed:782527}.
Function Catalyzes the decarboxylation of 3-octaprenyl-4-hydroxy benzoate to 2-octaprenylphenol. {ECO:0000269|PubMed:782527}.
Pathway Cofactor biosynthesis; ubiquinone biosynthesis. {ECO:0000269|PubMed:782527}.
Sequence Caution Sequence=AAA67639.1; Type=Frameshift; Positions=380; Evidence={ECO:0000305}; Sequence=CAA46146.1; Type=Frameshift; Positions=376; Evidence={ECO:0000305};
Similarity Belongs to the UbiD family. {ECO:0000305}.
Subcellular Location Cell membrane {ECO:0000305|PubMed:782527}; Peripheral membrane protein {ECO:0000305|PubMed:782527}.
Subunit Homohexamer. {ECO:0000305|PubMed:782527}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007752 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16131689 RefSeq NP_418285 497 3-octaprenyl-4-hydroxybenzoate decarboxylase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007752 proteins

Reference Database Accession Length Protein Name
GI:16131689 GenBank KHJ03984.1 497 3-octaprenyl-4-hydroxybenzoate carboxy-lyase [Escherichia coli]
GI:16131689 GenBank KHJ06291.1 497 3-octaprenyl-4-hydroxybenzoate carboxy-lyase [Escherichia coli]
GI:16131689 GenBank KHJ09440.1 497 3-octaprenyl-4-hydroxybenzoate carboxy-lyase [Escherichia coli]
GI:16131689 GenBank KHJ17172.1 497 3-octaprenyl-4-hydroxybenzoate carboxy-lyase [Escherichia coli]
GI:16131689 GenBank KHJ22110.1 497 3-octaprenyl-4-hydroxybenzoate carboxy-lyase [Escherichia coli]
GI:16131689 gnl IGS 497 3-octaprenyl-4-hydroxybenzoate decarboxylase [Escherichia coli ER2796]

Related Sequences to LMP007752 proteins

Reference Database Accession Length Protein Name
GI:16131689 GenBank EII43776.1 497 UbiD family decarboxylase [Escherichia coli 2.3916]
GI:16131689 GenBank EYX83398.1 497 3-octaprenyl-4-hydroxybenzoate carboxy-lyase [Escherichia coli O156:H25 str. 2011C-3602]
GI:16131689 GenBank EYZ19991.1 497 3-octaprenyl-4-hydroxybenzoate carboxy-lyase [Escherichia coli O103:H25 str. 2010C-4529]
GI:16131689 GenBank KHI01090.1 497 3-octaprenyl-4-hydroxybenzoate carboxy-lyase [Escherichia coli]
GI:16131689 RefSeq WP_000339802.1 497 3-polyprenyl-4-hydroxybenzoate decarboxylase [Escherichia coli]
GI:16131689 RefSeq WP_000339808.1 497 3-polyprenyl-4-hydroxybenzoate decarboxylase [Escherichia coli]