Gene/Proteome Database (LMPD)

LMPD ID
LMP007748
Gene ID
944811
Species
Escherichia coli K-12 (E. coli)
Gene Name
cyclopropane fatty acyl phospholipid synthase, SAM-dependent
Gene Symbol
cfa
Synonyms
ECK1657; JW1653; cdfA
Summary
Cfa binds a bicarbonate ion that is involved in catalysis. [More information is available at EcoGene: EG11531]. Cyclopropane fatty acyl phospholipid synthase (CFA) catalyzes a modification of the acyl chains of phospholipid bilayers through methylenation of unsaturated fatty acyl chains to their cyclopropane derivatives . [More information is available at EcoCyc: EG11531].
Orthologs
View orthologs and multiple alignments for cfa

Proteins

cyclopropane fatty acyl phospholipid synthase, SAM-dependent [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_416178
Protein GI 16129619
UniProt ID P0A9H7
Length 382
RefSeq Status REVIEWED
MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNPDFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGARLFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMICEKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTILLQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLNVDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPEIADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR

Gene Information

Entrez Gene ID
944811
Gene Name
cyclopropane fatty acyl phospholipid synthase, SAM-dependent
Gene Symbol
cfa
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0008825 IDA:EcoCyc F cyclopropane-fatty-acyl-phospholipid synthase activity
GO:0006633 IEA:UniProtKB-UniPathway P fatty acid biosynthetic process
GO:0030258 IDA:EcoCyc P lipid modification

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY0-541 cyclopropane fatty acid (CFA) biosynthesis
PWY0-541 cyclopropane fatty acid (CFA) biosynthesis
PWY0-541 cyclopropane fatty acid (CFA) biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR003333 Mycolic_cyclopropane_synthase
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase

UniProt Annotations

Entry Information

Gene Name
cyclopropane fatty acyl phospholipid synthase, SAM-dependent
Protein Entry
CFA_ECOLI
UniProt ID
P0A9H7
Species
E. coli

Comments

Comment Type Description
Catalytic Activity S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.
Enzyme Regulation Inhibited by sinefungin, A9145C and S-adenosyl- L-homocysteine. {ECO:0000269|PubMed:1445840}.
Function Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge. {ECO:0000250}.
Interaction P37024:hrpB; NbExp=1; IntAct=EBI-1124838, EBI-549226;
Pathway Lipid metabolism; fatty acid biosynthesis.
Similarity Belongs to the CFA/CMAS family. {ECO:0000305}.
Subcellular Location Cytoplasm.

Identical and Related Proteins

Unique RefSeq proteins for LMP007748 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16129619 RefSeq NP_416178 382 cyclopropane fatty acyl phospholipid synthase, SAM-dependent [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007748 proteins

Reference Database Accession Length Protein Name
GI:16129619 GenBank KHI15955.1 382 cyclopropane fatty acyl phospholipid synthase [Escherichia coli]
GI:16129619 GenBank KHI17797.1 382 cyclopropane fatty acyl phospholipid synthase [Escherichia coli]
GI:16129619 GenBank KHI24929.1 382 cyclopropane fatty acyl phospholipid synthase [Escherichia coli]
GI:16129619 GenBank KHI46422.1 382 cyclopropane fatty acyl phospholipid synthase [Escherichia coli]
GI:16129619 GenBank KHI60960.1 382 cyclopropane fatty acyl phospholipid synthase [Escherichia coli]
GI:16129619 gnl IGS 382 cyclopropane fatty acyl phospholipid synthase (unsaturated-phospholipid methyltransferase) [Escherichia coli ER2796]

Related Sequences to LMP007748 proteins

Reference Database Accession Length Protein Name
GI:16129619 GenBank KDY71212.1 382 cyclopropane-fatty-acyl-phospholipid synthase [Escherichia coli 2-460-02_S4_C2]
GI:16129619 GenBank KDY77966.1 382 cyclopropane-fatty-acyl-phospholipid synthase [Escherichia coli 2-460-02_S4_C3]
GI:16129619 GenBank KDZ38544.1 382 cyclopropane-fatty-acyl-phospholipid synthase [Escherichia coli 3-020-07_S3_C1]
GI:16129619 GenBank KEJ51140.1 382 cyclopropane-fatty-acyl-phospholipid synthase [Escherichia coli 2-460-02_S4_C1]
GI:16129619 GenBank KEJ68683.1 382 cyclopropane-fatty-acyl-phospholipid synthase [Escherichia coli 3-020-07_S3_C2]
GI:16129619 RefSeq WP_032182825.1 382 cyclopropane fatty acyl phospholipid synthase [Escherichia coli]