Gene/Proteome Database (LMPD)
LMPD ID
LMP007740
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
glycerol kinase
Gene Symbol
Synonyms
ECK3918; JW3897
Summary
The enzyme can undergo a reversible subunit dissociation between tetramer and dimer . [More information is available at EcoCyc: EG10398].
Orthologs
Proteins
| glycerol kinase [Escherichia coli str. K-12 substr. MG1655] | |
|---|---|
| Refseq ID | NP_418361 |
| Protein GI | 16131764 |
| UniProt ID | P0A6F3 |
| Length | 502 |
| RefSeq Status | REVIEWED |
| MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | IDA:UniProtKB | C | cytosol |
| GO:0005524 | IEA:UniProtKB-HAMAP | F | ATP binding |
| GO:0004370 | IDA:UniProtKB | F | glycerol kinase activity |
| GO:0046872 | IDA:EcoCyc | F | metal ion binding |
| GO:0008270 | IDA:UniProtKB | F | zinc ion binding |
| GO:0006974 | IEP:EcoliWiki | P | cellular response to DNA damage stimulus |
| GO:0019563 | IEA:UniProtKB-UniPathway | P | glycerol catabolic process |
| GO:0006071 | IDA:UniProtKB | P | glycerol metabolic process |
| GO:0006072 | IEA:UniProtKB-HAMAP | P | glycerol-3-phosphate metabolic process |
| GO:0016310 | IDA:EcoCyc | P | phosphorylation |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| eco00561 | Glycerolipid metabolism |
| ko00561 | Glycerolipid metabolism |
| eco01100 | Metabolic pathways |
BIOCYC Pathway Links
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=1.3 uM for glycerol {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; KM=400 uM for D-glyceraldehyde {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; KM=500 uM for dihydroxyacetone {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; KM=3 mM for L-glyceraldehyde {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; KM=4 mM for ATP {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; pH dependence: Optimum pH is 9.8. {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; |
| Catalytic Activity | ATP + glycerol = ADP + sn-glycerol 3- phosphate. {ECO:0000255|HAMAP-Rule:MF_00186, ECO:0000269|PubMed:5335908}. |
| Enzyme Regulation | Activity of this regulatory enzyme is affected by several metabolites. The non-competitive allosteric inhibition by fructose 1,6-bisphosphate (FBP) causes alterations in the quaternary structure of the enzyme. FBP inhibition requires that the enzyme exist only in a tetrameric state. Salt such as KCl reduces the affinity of the tetrameric form of the enzyme for FBP. Unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system, also inhibits non-competitively and allostericaly the activity. Unlike FBP, both the dimer and the tetramer appear to be fully sensitive to enzyme EIIA-Glc inhibition. Zn(+2) greatly enhances the inhibitory potency of EIIA-Glc. Both allosteric regulatory agents is strongly pH dependent, with maximal inhibition occurring at pH 6.5. {ECO:0000269|PubMed:215195, ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:31903, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5328677, ECO:0000269|PubMed:8430315, ECO:0000269|PubMed:9817843, ECO:0000269|PubMed:9843423}. |
| Function | Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn- glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP. {ECO:0000255|HAMAP-Rule:MF_00186, ECO:0000269|PubMed:2826434, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908}. |
| Induction | By L-alpha-glycerol 3-phosphate. {ECO:0000269|PubMed:2826434}. |
| Pathway | Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00186}. |
| Similarity | Belongs to the FGGY kinase family. {ECO:0000255|HAMAP- Rule:MF_00186}. |
| Subunit | Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc (crr). Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization. {ECO:0000255|HAMAP-Rule:MF_00186, ECO:0000269|PubMed:10090737, ECO:0000269|PubMed:17441732, ECO:0000269|PubMed:215194, ECO:0000269|PubMed:215195, ECO:0000269|PubMed:4934840, ECO:0000269|PubMed:8170944, ECO:0000269|PubMed:8430315, ECO:0000269|PubMed:9817843, ECO:0000269|PubMed:9843423}. |
| Web Resource | Name=Worthington enzyme manual; URL="http://www.worthington-biochem.com/GKEC/"; |
Identical and Related Proteins
Unique RefSeq proteins for LMP007740 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 16131764 | RefSeq | NP_418361 | 502 | glycerol kinase [Escherichia coli str. K-12 substr. MG1655] |
Identical Sequences to LMP007740 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16131764 | GenBank | KHJ01009.1 | 502 | glycerol kinase [Escherichia coli] |
| GI:16131764 | GenBank | KHJ03511.1 | 502 | glycerol kinase [Escherichia coli] |
| GI:16131764 | GenBank | KHJ05209.1 | 502 | glycerol kinase [Escherichia coli] |
| GI:16131764 | GenBank | KHJ11345.1 | 502 | glycerol kinase [Escherichia coli] |
| GI:16131764 | GenBank | KHJ20039.1 | 502 | glycerol kinase [Escherichia coli] |
| GI:16131764 | gnl | IGS | 502 | glycerol kinase [Escherichia coli ER2796] |
Related Sequences to LMP007740 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16131764 | GenBank | ESA61829.1 | 509 | glycerol kinase [Escherichia coli 110957] |
| GI:16131764 | GenBank | ESA64832.1 | 509 | glycerol kinase [Escherichia coli 113303] |
| GI:16131764 | GenBank | ESA77371.1 | 509 | glycerol kinase [Escherichia coli 907357] |
| GI:16131764 | GenBank | ESA90841.1 | 509 | glycerol kinase [Escherichia coli 907713] |
| GI:16131764 | GenBank | ESC92625.1 | 509 | glycerol kinase [Escherichia coli 113302] |
| GI:16131764 | RefSeq | NP_290555.1 | 509 | glycerol kinase [Escherichia coli O157:H7 str. EDL933] |