Gene/Proteome Database (LMPD)

LMPD ID
LMP007730
Gene ID
946665
Species
Escherichia coli K-12 (E. coli)
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Gene Symbol
fabF
Synonyms
ECK1081; JW1081; cvc; fabJ; vtr
Summary
There are three -ketoacyl-ACP synthases (KAS) in Escherichia coli: |FRAME: [More information is available at EcoCyc: EG12606].
Orthologs
View orthologs and multiple alignments for fabF

Proteins

3-oxoacyl-[acyl-carrier-protein] synthase II [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_415613
Protein GI 16129058
UniProt ID P0AAI5
Length 413
RefSeq Status REVIEWED
MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTSAYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIGAAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISIATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQAASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPENGAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSSTKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGMEYTLCNSFGFGGTNGSLIFKKI

Gene Information

Entrez Gene ID
946665
Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Gene Symbol
fabF
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:UniProtKB C cytosol
GO:0004315 IDA:EcoCyc F 3-oxoacyl-[acyl-carrier-protein] synthase activity
GO:0033817 IDA:CACAO F beta-ketoacyl-acyl-carrier-protein synthase II activity
GO:0006633 IEA:UniProtKB-UniPathway P fatty acid biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco00780 Biotin metabolism
ko00780 Biotin metabolism
eco00061 Fatty acid biosynthesis
ko00061 Fatty acid biosynthesis
M00083 Fatty acid biosynthesis, elongation
eco_M00083 Fatty acid biosynthesis, elongation
eco01212 Fatty acid metabolism
ko01212 Fatty acid metabolism
eco01100 Metabolic pathways
M00572 Pimeloyl-ACP biosynthesis, BioC-BioH pathway, malonyl-ACP => pimeloyl-ACP
eco_M00572 Pimeloyl-ACP biosynthesis, BioC-BioH pathway, malonyl-ACP => pimeloyl-ACP

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY-6519 7-keto-8-aminopelargonate biosynthesis I
BIOTIN-BIOSYNTHESIS-PWY biotin biosynthesis I
PWY-5973 cis-vaccenate biosynthesis
PWY-5973 cis-vaccenate biosynthesis
PWY-5966 fatty acid biosynthesis initiation II
PWY-5966 fatty acid biosynthesis initiation II
PWY-5966 fatty acid biosynthesis initiation II
PWY-5965 fatty acid biosynthesis initiation III
PWY-5965 fatty acid biosynthesis initiation III
PWY-5965 fatty acid biosynthesis initiation III
FASYN-ELONG-PWY fatty acid elongation -- saturated
FASYN-ELONG-PWY fatty acid elongation -- saturated
FASYN-ELONG-PWY fatty acid elongation -- saturated
PWY0-881 superpathway of fatty acid biosynthesis I (E. coli)
PWY0-881 superpathway of fatty acid biosynthesis I (E. coli)
FASYN-INITIAL-PWY superpathway of fatty acid biosynthesis initiation (E. coli)
FASYN-INITIAL-PWY superpathway of fatty acid biosynthesis initiation (E. coli)
FASYN-INITIAL-PWY superpathway of fatty acid biosynthesis initiation (E. coli)
PWY-6285 superpathway of fatty acids biosynthesis (E. coli)
PWY-6284 superpathway of unsaturated fatty acids biosynthesis (E. coli)
PWY-6284 superpathway of unsaturated fatty acids biosynthesis (E. coli)

Domain Information

InterPro Annotations

Accession Description
IPR017568 3-oxoacyl-[acyl-carrier-protein] synthase 2
IPR014031 Beta-ketoacyl synthase, C-terminal
IPR014030 Beta-ketoacyl synthase, N-terminal
IPR018201 Beta-ketoacyl synthase, active site
IPR016039 Thiolase-like
IPR016038 Thiolase-like, subgroup

UniProt Annotations

Entry Information

Gene Name
3-oxoacyl-[acyl-carrier-protein] synthase II
Protein Entry
FABF_ECOLI
UniProt ID
P0AAI5
Species
E. coli

Comments

Comment Type Description
Catalytic Activity (Z)-hexadec-11-enoyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = (Z)-3-oxooctadec-13-enoyl-[acyl- carrier-protein] + CO(2) + [acyl-carrier-protein]. {ECO:0000269|PubMed:9013860}.
Function Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Has a preference for short chain acid substrates and may function to supply the octanoic substrates for lipoic acid biosynthesis. {ECO:0000269|PubMed:6988423, ECO:0000269|PubMed:9013860}.
Interaction P0A6Y8:dnaK; NbExp=2; IntAct=EBI-542783, EBI-542092;
Pathway Lipid metabolism; fatty acid biosynthesis.
Similarity Belongs to the beta-ketoacyl-ACP synthases family. {ECO:0000305}.
Subunit Homodimer.

Identical and Related Proteins

Unique RefSeq proteins for LMP007730 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16129058 RefSeq NP_415613 413 3-oxoacyl-[acyl-carrier-protein] synthase II [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007730 proteins

Reference Database Accession Length Protein Name
GI:16129058 GenBank KHI94543.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 GenBank KHJ05630.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 GenBank KHJ10637.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 GenBank KHJ17522.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 GenBank KHJ22766.1 413 3-oxoacyl-ACP synthase [Escherichia coli]
GI:16129058 gnl IGS 413 3-oxoacyl-[acyl-carrier-protein] synthase II [Escherichia coli ER2796]

Related Sequences to LMP007730 proteins

Reference Database Accession Length Protein Name
GI:16129058 GenBank ETY51408.1 413 3-oxoacyl-[acyl-carrier-protein] synthase 2 [Escherichia coli BIDMC 49b]
GI:16129058 GenBank ETY54128.1 413 3-oxoacyl-[acyl-carrier-protein] synthase 2 [Escherichia coli BIDMC 49a]
GI:16129058 GenBank EYY26402.1 413 3-oxoacyl-ACP synthase [Escherichia coli O121:H19 str. 2011C-3072]
GI:16129058 gnl mgcchina 413 3-oxoacyl-[acyl-carrier-protein] synthase II [Shigella sonnei Ss046]
GI:16129058 RefSeq WP_000044674.1 413 3-oxoacyl-ACP synthase [Shigella sonnei]
GI:16129058 RefSeq WP_032140064.1 413 3-oxoacyl-ACP synthase [Escherichia coli]