Gene/Proteome Database (LMPD)
LMPD ID
LMP007717
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
fused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase
Gene Symbol
Synonyms
ECK3838; JW3822; oldB
Summary
FadR regulon. [More information is available at EcoGene: EG10279]. The alpha subunit has four enzymatic activities associated with it. [More information is available at EcoCyc: EG10279].
Orthologs
Proteins
| fused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli str. K-12 substr. MG1655] | |
|---|---|
| Refseq ID | NP_418288 |
| Protein GI | 16131692 |
| UniProt ID | P21177 |
| Length | 729 |
| RefSeq Status | REVIEWED |
| MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIGVLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLPVPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGADSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPKLEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKSFVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAWKGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDRVDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMHFFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFAGFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQKDYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSEEEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAKYLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA | |
Gene Information
Entrez Gene ID
Gene Name
fused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase
Gene Symbol
Species
Escherichia coli K-12
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0036125 | IEA:InterPro | C | fatty acid beta-oxidation multienzyme complex |
| GO:0003857 | IDA:UniProtKB | F | 3-hydroxyacyl-CoA dehydrogenase activity |
| GO:0008692 | IDA:UniProtKB | F | 3-hydroxybutyryl-CoA epimerase activity |
| GO:0050662 | IEA:InterPro | F | coenzyme binding |
| GO:0004165 | IDA:UniProtKB | F | dodecenoyl-CoA delta-isomerase activity |
| GO:0004300 | IDA:UniProtKB | F | enoyl-CoA hydratase activity |
| GO:0006635 | IDA:EcoCyc | P | fatty acid beta-oxidation |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| eco01110 | Biosynthesis of secondary metabolites |
| eco01040 | Biosynthesis of unsaturated fatty acids |
| ko01040 | Biosynthesis of unsaturated fatty acids |
| eco00650 | Butanoate metabolism |
| ko00650 | Butanoate metabolism |
| eco00930 | Caprolactam degradation |
| ko00930 | Caprolactam degradation |
| eco01200 | Carbon metabolism |
| ko01200 | Carbon metabolism |
| eco00071 | Fatty acid degradation |
| ko00071 | Fatty acid degradation |
| eco01212 | Fatty acid metabolism |
| ko01212 | Fatty acid metabolism |
| eco00281 | Geraniol degradation |
| ko00281 | Geraniol degradation |
| eco00903 | Limonene and pinene degradation |
| ko00903 | Limonene and pinene degradation |
| eco00310 | Lysine degradation |
| ko00310 | Lysine degradation |
| eco01100 | Metabolic pathways |
| eco01120 | Microbial metabolism in diverse environments |
| eco00640 | Propanoate metabolism |
| ko00640 | Propanoate metabolism |
| eco00380 | Tryptophan metabolism |
| ko00380 | Tryptophan metabolism |
| eco00280 | Valine, leucine and isoleucine degradation |
| ko00280 | Valine, leucine and isoleucine degradation |
| eco00410 | beta-Alanine metabolism |
| ko00410 | beta-Alanine metabolism |
| M00087 | beta-Oxidation |
| eco_M00087 | beta-Oxidation |
BIOCYC Pathway Links
| BIOCYC Pathway ID | Description |
|---|---|
| FAO-PWY | fatty acid beta-oxidation I |
| FAO-PWY | fatty acid beta-oxidation I |
| FAO-PWY | fatty acid beta-oxidation I |
| PWY-5136 | fatty acid beta-oxidation II (core pathway) |
| PWY-5137 | fatty acid beta-oxidation III (unsaturated, odd number) |
| PWY-5137 | fatty acid beta-oxidation III (unsaturated, odd number) |
| PWY-7007 | methyl ketone biosynthesis |
| PWY0-1337 | oleate beta-oxidation |
| PWY0-1337 | oleate beta-oxidation |
| PWY-561 | superpathway of glyoxylate cycle |
| VALDEG-PWY | valine degradation I |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR006108 | 3-hydroxyacyl-CoA dehydrogenase, C-terminal |
| IPR006176 | 3-hydroxyacyl-CoA dehydrogenase, NAD binding |
| IPR006180 | 3-hydroxyacyl-CoA dehydrogenase, conserved site |
| IPR008927 | 6-phosphogluconate dehydrogenase, C-terminal-like |
| IPR029045 | ClpP/crotonase-like domain |
| IPR001753 | Crotonase_core_superfam |
| IPR013328 | Dehydrogenase, multihelical |
| IPR018376 | Enoyl-CoA hydratase/isomerase, conserved site |
| IPR012799 | FadB |
| IPR016040 | NAD(P)-binding domain |
UniProt Annotations
Entry Information
Gene Name
fused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase
Protein Entry
FADB_ECOLI
UniProt ID
Species
E. coli
Comments
| Comment Type | Description |
|---|---|
| Biophysicochemical Properties | Kinetic parameters: KM=53 uM for crotonyl-CoA (for enoyl-CoA hydratase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=8.7 mM for L-3-hydroxy-4-trans-decenoyl-CoA (for enoyl-CoA hydratase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=38 mM for D-3-hydroxy-4-trans-decenoyl-CoA (for enoyl-CoA hydratase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=5.8 uM for 3-cis-tetradecenoyl-CoA (for Delta(3)-cis- Delta(2)-trans-enoyl-CoA isomerase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=69 uM for acetoacetyl-CoA (for 3-hydroxyacyl-CoA dehydrogenase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; KM=2.0 uM for NADH (for 3-hydroxyacyl-CoA dehydrogenase activity) {ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}; |
| Catalytic Activity | (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O. {ECO:0000255|HAMAP-Rule:MF_01621}. |
| Catalytic Activity | (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl- CoA. {ECO:0000255|HAMAP-Rule:MF_01621}. |
| Catalytic Activity | (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH. {ECO:0000255|HAMAP-Rule:MF_01621}. |
| Catalytic Activity | (S)-3-hydroxybutanoyl-CoA = (R)-3- hydroxybutanoyl-CoA. {ECO:0000255|HAMAP-Rule:MF_01621}. |
| Function | Involved in the aerobic and anaerobic degradation of long-chain fatty acids via beta-oxidation cycle. Catalyzes the formation of 3-oxoacyl-CoA from enoyl-CoA via L-3-hydroxyacyl-CoA. It can also use D-3-hydroxyacyl-CoA and cis-3-enoyl-CoA as substrate. {ECO:0000255|HAMAP-Rule:MF_01621, ECO:0000269|PubMed:12535077, ECO:0000269|PubMed:1748662, ECO:0000269|PubMed:368024, ECO:0000269|PubMed:8454629, ECO:0000269|PubMed:8755745}. |
| Induction | Repressed by FadR in the absence of LCFAs (fatty acids of, at least, 12 carbon atoms). When LCFAs are present in the medium, they are converted to long-chain acyl-CoAs which bind to FadR resulting in its release from the DNA and thus derepression of the transcription. |
| Pathway | Lipid metabolism; fatty acid beta-oxidation. {ECO:0000255|HAMAP-Rule:MF_01621}. |
| Similarity | In the C-terminal section; belongs to the 3- hydroxyacyl-CoA dehydrogenase family. {ECO:0000255|HAMAP- Rule:MF_01621}. |
| Similarity | In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000255|HAMAP-Rule:MF_01621}. |
| Subunit | Heterotetramer of two alpha chains (FadB) and two beta chains (FadA). {ECO:0000255|HAMAP-Rule:MF_01621, ECO:0000269|PubMed:368024}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007717 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 16131692 | RefSeq | NP_418288 | 729 | fused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli str. K-12 substr. MG1655] |
Identical Sequences to LMP007717 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16131692 | GenBank | KHH73488.1 | 729 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
| GI:16131692 | GenBank | KHH97108.1 | 729 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
| GI:16131692 | GenBank | KHI15029.1 | 729 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
| GI:16131692 | GenBank | KHI17356.1 | 729 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
| GI:16131692 | GenBank | KHI53370.1 | 729 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
| GI:16131692 | gnl | IGS | 729 | fused 3-hydroxybutyryl-CoA epimerase/delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase/enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli ER2796] |
Related Sequences to LMP007717 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:16131692 | GenBank | EFJ67713.1 | 771 | fatty oxidation complex, alpha subunit FadB [Escherichia coli MS 175-1] |
| GI:16131692 | GenBank | EFK13896.1 | 771 | fatty oxidation complex, alpha subunit FadB [Escherichia coli MS 116-1] |
| GI:16131692 | GenBank | EFK90482.1 | 771 | fatty oxidation complex, alpha subunit FadB [Escherichia coli MS 146-1] |
| GI:16131692 | GenBank | EGI08485.1 | 771 | fatty oxidation complex, alpha subunit FadB [Escherichia coli H736] |
| GI:16131692 | GenBank | EIG68973.1 | 771 | fatty acid oxidation complex subunit alpha [Escherichia sp. 4_1_40B] |
| GI:16131692 | GenBank | ESE06860.1 | 771 | fatty oxidation complex, alpha subunit FadB [Escherichia coli 908658] |