Gene/Proteome Database (LMPD)

LMPD ID
LMP007706
Gene ID
945217
Species
Escherichia coli K-12 (E. coli)
Gene Name
octanoyltransferase; octanoyl-[ACP]:protein N-octanoyltransferase
Gene Symbol
lipB
Synonyms
ECK0623; JW5089; cde
Summary
Revised TTG start is required for active protein. [More information is available at EcoGene: EG11591]. LipB is a lipoyl-protein ligase that specifically utilizes LipA-generated lipoyl-ACP, in contrast to the other lipoyl-protein ligase, LplA, which utilizes lipoate imported from outside the cell . [More information is available at EcoCyc: EG11591].
Orthologs
View orthologs and multiple alignments for lipB

Proteins

octanoyltransferase; octanoyl-[ACP]:protein N-octanoyltransferase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_415163
Protein GI 90111156
UniProt ID P60720
Length 213
RefSeq Status REVIEWED
MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHYPVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDLSPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA

Gene Information

Entrez Gene ID
945217
Gene Name
octanoyltransferase; octanoyl-[ACP]:protein N-octanoyltransferase
Gene Symbol
lipB
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-HAMAP C cytoplasm
GO:0003824 IDA:EcoliWiki F catalytic activity
GO:0033819 IEA:UniProtKB-EC F lipoyl(octanoyl) transferase activity
GO:0017118 IDA:EcoliWiki F lipoyltransferase activity
GO:0016415 IEA:InterPro F octanoyltransferase activity
GO:0016740 IDA:EcoliWiki F transferase activity
GO:0006464 IDA:EcoliWiki P cellular protein modification process
GO:0009107 IMP:EcoliWiki P lipoate biosynthetic process
GO:0009106 IMP:EcoliWiki P lipoate metabolic process
GO:0010629 IDA:EcoliWiki P negative regulation of gene expression

KEGG Pathway Links

KEGG Pathway ID Description
eco00785 Lipoic acid metabolism
ko00785 Lipoic acid metabolism
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY0-501 lipoate biosynthesis and incorporation I
PWY0-501 lipoate biosynthesis and incorporation I
PWY0-501 lipoate biosynthesis and incorporation I

Domain Information

InterPro Annotations

Accession Description
IPR004143 Biotin/lipoate A/B protein ligase
IPR000544 Octanoyltransferase
IPR020605 Octanoyltransferase, conserved site

UniProt Annotations

Entry Information

Gene Name
octanoyltransferase; octanoyl-[ACP]:protein N-octanoyltransferase
Protein Entry
LIPB_ECOLI
UniProt ID
P60720
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=10.2 uM for octanoyl-ACP {ECO:0000269|PubMed:15642479}; KM=13.2 uM for apo-H protein {ECO:0000269|PubMed:15642479}; pH dependence: Optimum pH is about 7.5. {ECO:0000269|PubMed:15642479};
Catalytic Activity Octanoyl-[acyl-carrier-protein] + protein = protein N(6)-(octanoyl)lysine + [acyl-carrier-protein]. {ECO:0000255|HAMAP-Rule:MF_00013}.
Function Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate. {ECO:0000255|HAMAP-Rule:MF_00013, ECO:0000269|PubMed:15642479, ECO:0000269|PubMed:16342964}.
Mass Spectrometry Mass=23880.45; Mass_error=1.31; Method=Electrospray; Range=1-213; Evidence={ECO:0000269|PubMed:15642479};
Miscellaneous In the reaction, the free carboxyl group of octanoic acid is attached via an amide linkage to the epsilon- amino group of a specific lysine residue of lipoyl domains of lipoate-dependent enzymes.
Pathway Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier- protein]: step 1/2. {ECO:0000255|HAMAP-Rule:MF_00013}.
Sequence Caution Sequence=AAA66342.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAB40830.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Similarity Belongs to the LipB family. {ECO:0000255|HAMAP- Rule:MF_00013}.
Similarity Contains 1 BPL/LPL catalytic domain. {ECO:0000255|PROSITE-ProRule:PRU01067}.
Subcellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00013}.
Subunit Monomer or homotrimer. Both forms are active. {ECO:0000269|PubMed:15642479}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007706 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
90111156 RefSeq NP_415163 213 octanoyltransferase; octanoyl-[ACP]:protein N-octanoyltransferase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007706 proteins

Reference Database Accession Length Protein Name
GI:90111156 GenBank KHI99841.1 213 lipoate--protein ligase [Escherichia coli]
GI:90111156 GenBank KHJ07802.1 213 lipoate--protein ligase [Escherichia coli]
GI:90111156 GenBank KHJ11082.1 213 lipoate--protein ligase [Escherichia coli]
GI:90111156 GenBank KHJ16924.1 213 lipoate--protein ligase [Escherichia coli]
GI:90111156 GenBank KHJ28882.1 213 lipoate--protein ligase [Escherichia coli]
GI:90111156 gnl IGS 213 octanoyltransferase; octanoyl-[ACP]:protein N-octanoyltransferase [Escherichia coli ER2796]

Related Sequences to LMP007706 proteins

Reference Database Accession Length Protein Name
GI:90111156 GenBank EQP78304.1 213 octanoyltransferase [Escherichia coli HVH 79 (4-2512823)]
GI:90111156 GenBank EQT63532.1 213 octanoyltransferase [Escherichia coli HVH 188 (4-2356988)]
GI:90111156 GenBank EYZ96386.1 213 lipoate--protein ligase [Escherichia coli O119:H4 str. 03-3458]
GI:90111156 GenBank KDX52567.1 213 lipoyl(octanoyl) transferase [Escherichia coli 2-177-06_S4_C1]
GI:90111156 RefSeq WP_024176414.1 213 lipoate--protein ligase [Escherichia coli]
GI:90111156 RefSeq WP_024238258.1 213 lipoate--protein ligase [Escherichia coli]